Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,516,197 C→A 11.3% P21Q (CCG→CAG)  ate → arginyl‑tRNA‑‑protein transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,516,1970CA11.3% 113.1 / 3.7 42P21Q (CCG→CAG) atearginyl‑tRNA‑‑protein transferase
Reads supporting (aligned to +/- strand):  ref base C (21/16);  new base A (3/2);  total (24/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGCCTGCGTGCCG  >  NC_002947/4516052‑4516342
                                                                                                                                                  |                                                                                                                                                 
gCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCg                                                                                                                                                  >  2:57586/1‑147 (MQ=255)
    aaCAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGAGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACATGCCGgaggag                                                                                                                                            >  4:279582/1‑149 (MQ=255)
      cAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTTCGGCAGGTAGCTGCAggagtgggg                                                                                                                                          <  8:136701/149‑6 (MQ=255)
            ttCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCg                                                                                                                                      >  3:215611/1‑147 (MQ=255)
            ttCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCg                                                                                                                                      <  4:215611/147‑1 (MQ=255)
              cATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCa                                                                                                                                  >  3:318988/1‑149 (MQ=255)
               aTGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCa                                                                                                                                  <  6:111933/148‑1 (MQ=255)
                   gTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTg                                                                                                                             <  3:11760/149‑1 (MQ=255)
                        cTAGGCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTcc                                                                                                                         >  2:312146/1‑149 (MQ=255)
                         tAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGACGGAGGAGCAGGCGACCACGCTGTTCCTc                                                                                                                       >  8:275665/1‑149 (MQ=255)
                           ggCGAGTTCCCGTAGCTGGCTTACACTTAAAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTc                                                                                                                       <  6:284884/148‑1 (MQ=255)
                           ggCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGa                                                                                                                     <  4:318988/149‑1 (MQ=255)
                               aGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGc                                                                                                                                           >  4:152201/1‑123 (MQ=255)
                               aGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGc                                                                                                                                           <  3:152201/123‑1 (MQ=255)
                                         gCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggagga                                                                                                                                             >  7:290482/1‑111 (MQ=255)
                                         gCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCggagga                                                                                                                                             <  8:290482/111‑1 (MQ=255)
                                               ttACACAA‑AAAAAAAACTCTCACCAAACGGGTTGATCATGACACACTTCCCGCGCTTGAAGATCTATTCCACTCACACACACTCATGCAGCAACCTGCCCGAGGAGCAGCCGACCACGCTGTTCCTCGACCCCACCCAGCCGATCGACg                                                                                                 >  4:106627/1‑149 (MQ=255)
                                                   acTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTACTGCAGCTACCTGCAGGAGGAGCAGGCGACCCCGCTGTTCCTCGACCCCGGCCAGCCGATGGACGTGCa                                                                                             >  7:135921/1‑149 (MQ=255)
                                                     tt‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACg                                                                                           >  8:68894/1‑149 (MQ=255)
                                                     tt‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACg                                                                                           >  1:328621/1‑149 (MQ=255)
                                                     tt‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACg                                                                                           >  2:34809/1‑149 (MQ=255)
                                                       aaaaaaaaaGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACg                                                                                           <  1:312146/147‑1 (MQ=255)
                                                                 tctcACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGAt                                                                                >  2:261350/1‑149 (MQ=255)
                                                                 tctcACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACATGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGAt                                                                                >  4:252313/1‑149 (MQ=255)
                                                                      ccGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTc                                                                           >  7:29410/1‑149 (MQ=255)
                                                                       cGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCg                                                                          >  1:147046/1‑149 (MQ=255)
                                                                              gTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATgg                                                                   <  7:139409/149‑1 (MQ=255)
                                                                              gTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATgg                                                                   >  1:303020/1‑149 (MQ=255)
                                                                               ttGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATggg                                                                  <  1:57586/149‑1 (MQ=255)
                                                                                      tGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTcgc                                                           <  2:147046/149‑1 (MQ=255)
                                                                                          agagTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCa                                                       <  2:328621/149‑1 (MQ=255)
                                                                                              ttGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTg                                                    <  2:303020/148‑1 (MQ=255)
                                                                                                 gcgcGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGa                                                <  7:68894/149‑1 (MQ=255)
                                                                                                   gcgGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTACGCCGCAGTGGCGac                                               >  3:199305/1‑148 (MQ=255)
                                                                                                        tGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGc                                                  <  6:75925/140‑1 (MQ=255)
                                                                                                        tGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGc                                                  >  5:75925/1‑140 (MQ=255)
                                                                                                                            ccccACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGaa                     >  1:76246/1‑149 (MQ=255)
                                                                                                                            ccccACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGaa                     <  3:279582/149‑1 (MQ=255)
                                                                                                                                 cTCCGGCAGGTAGCTGCAGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTg                           <  3:241676/138‑1 (MQ=255)
                                                                                                                                 cTCCGGCAGGTAGCTGCAGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTg                           >  4:241676/1‑138 (MQ=255)
                                                                                                                                  tCCGGCAGGTATCTGCAGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGACAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGTGCTTTCGCCTCAGTGGCGACCACCTTTACCGCCCAAACTGCCAGAACTGCa                >  8:39071/1‑148 (MQ=255)
                                                                                                                                    cTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACg             >  6:37076/1‑149 (MQ=255)
                                                                                                                                                 ccGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGCCTGCGTGCCg  <  1:34809/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCCCAACAGCGCTTCATGGGTTGCCTAGGCGAGTTCCCGTAGCTGGCTTACACTT‑AAAAAAAAGTCTCACCGAAGGGGTTGATCATGACAGAGTTGGCGCGGTTGAAGTTCTATGCCACTCAGCCCCACTCCTGCAGCTACCTGCCGGAGGAGCAGGCGACCACGCTGTTCCTCGACCCCAGCCAGCCGATGGACGTGCACGTGTACGCCGATTTGTCGGAAATGGGCTTTCGCCGCAGTGGCGACCACCTGTACCGCCCACACTGCCAGAACTGCAACGCCTGCGTGCCG  >  NC_002947/4516052‑4516342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑