Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,530,025 T→A 8.9% intergenic (+31/‑137) PP_4018 → / → topB acetyltransferase/DNA topoisomerase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,530,0250TA 8.9% 117.2 / 3.6 45intergenic (+31/‑137)PP_4018/topBacetyltransferase/DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base T (25/16);  new base A (2/2);  total (27/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.30e-01

AGCGCCCAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCC  >  NC_002947/4529878‑4530168
                                                                                                                                                    |                                                                                                                                               
aGCGCCCAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGtt                                                                                                                                                 <  6:214989/148‑1 (MQ=255)
  cgcCCAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTAc                                                                                                                                              <  8:6038/149‑1 (MQ=255)
   gcCCAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTAcc                                                                                                                                             <  6:319415/149‑1 (MQ=255)
     ccAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCcg                                                                                                                                           >  6:321647/1‑149 (MQ=255)
            cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGAagag                                                                                                                                    <  2:66294/149‑1 (MQ=255)
            cacgccacgcCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTAAACCCGCGAaga                                                                                                                                     <  4:74101/148‑1 (MQ=255)
                 cacgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCTT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCgg                                                                                                                               <  4:206660/149‑1 (MQ=255)
                  acgccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGc                                                                                                                              >  5:11755/1‑149 (MQ=255)
                    gccGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGAGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGAACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGACGGCt                                                                                                                             >  7:4458/1‑148 (MQ=255)
                           tATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACACGCGAAGAGGCCGGCTCAGGCGa                                                                                                                      >  1:223185/1‑148 (MQ=255)
                                gCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATAc                                                                                                                 >  6:106692/1‑148 (MQ=255)
                                  ggCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCg                                                                                                                                >  6:110810/1‑131 (MQ=255)
                                  ggCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCg                                                                                                                                <  5:110810/131‑1 (MQ=255)
                                   gCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATcc                                                                                                             >  7:37590/1‑149 (MQ=255)
                                     tGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCgg                                                                                                                               <  6:165477/129‑1 (MQ=255)
                                     tGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCgg                                                                                                                               >  5:165477/1‑129 (MQ=255)
                                       ggCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATcccccc                                                                                                         >  2:172780/1‑147 (MQ=255)
                                                      aGCGAGGAATTCCTCGAAGCTGGGATCCCGCATGTCGACATGGTGCGCGACTCGCGCGGCTGATCCAT‑CACCTGCACTGGACTCTTCGCGGGTTTACCGGCGAAGAGGGGGGGTCAGGCGATATACCTCCGGGCGGCCAAGAAGCCTGt                                                                                          >  4:241011/1‑149 (MQ=255)
                                                          aGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcat                                                                                      <  5:321647/149‑1 (MQ=255)
                                                          aGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcat                                                                                      >  1:22116/1‑149 (MQ=255)
                                                                  cTCGACGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCGTTATCCAT‑CACCTCCACTGGCCTCTTCGCGGGTTAACCGGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAc                                                                                        <  6:95760/139‑1 (MQ=255)
                                                                  cTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcatccatc                                                                                 >  7:8310/1‑146 (MQ=255)
                                                                  cTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAc                                                                                        >  5:95760/1‑139 (MQ=255)
                                                                   tCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCAGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTAcatccatc                                                                                 >  1:94374/1‑145 (MQ=255)
                                                                          tGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTAGCGGGGTTAACCGGGAAGAGGGCGGGTCAGGCGCTATAACACCCCGCTGAGCAAAAACCAGTTCAAACCTCCACaaaaaaat                                                                      >  3:12093/1‑147 (MQ=255)
                                                                            gCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACatccatcc                                                                                <  4:249450/137‑1 (MQ=255)
                                                                            gCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACatccatcc                                                                                >  3:249450/1‑137 (MQ=255)
                                                                               tCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCct                                                                 <  2:22116/149‑1 (MQ=255)
                                                                                   gCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTgc                                                             <  6:141798/149‑1 (MQ=255)
                                                                                                gagcgcGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCATCGGGCGGTTTCCCGCGAACAGGGCCGCTAAGGCCATATACAACCCCGCTGAACAAAAACCAGTAAAACCCAGCAGATAAAACATGCCAATGCGCCACCCCTGgc                                                 >  2:169750/3‑148 (MQ=255)
                                                                                                cggcgcTACCCGGGCGCCTCA‑CCATCCACCTCCACTGCCCACTTCGCGGTTTTCCCCGAGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTgc                                                 <  1:169750/146‑1 (MQ=255)
                                                                                                     cgACTCGCGCGCCTGATCCAT‑CACGTGCACTGGCCTTTTCGGGGTTTTACCCGCTAATTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATcc                                           <  8:37590/149‑1 (MQ=255)
                                                                                                         tCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATg                                       >  7:219336/1‑149 (MQ=255)
                                                                                                           cggcgcCTTATCCAT‑CACCTGCACTGCCCTCTTCGCGGTTTTACCCGCTAATAGGCCGGCTCAGGCGATATACATCCCCTCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATgg                                      <  8:4458/146‑1 (MQ=255)
                                                                                                                       cAT‑CCCCTGCATTGGCCTCTTCGGGGTTTTACCCGGTAAGTGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGa                         <  7:201981/149‑1 (MQ=255)
                                                                                                                       cAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGa                         >  2:36815/1‑149 (MQ=255)
                                                                                                                        aT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCCCCCCGAt                        >  5:261619/1‑149 (MQ=255)
                                                                                                                        aT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGAt                        >  5:89542/1‑149 (MQ=255)
                                                                                                                              gtccACTGGCATCTTCGCGTTTTAACCCGCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGc                   <  5:106692/146‑1 (MQ=255)
                                                                                                                                      gCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTg                                                  >  5:127023/1‑110 (MQ=255)
                                                                                                                                       ccTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCCCCCCCCCGAGGAAGCGTCCCCGGc          >  5:329845/1‑149 (MQ=255)
                                                                                                                                        ctctTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGc          >  3:306982/1‑148 (MQ=255)
                                                                                                                                         tctTCGCGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCa        >  4:19022/1‑149 (MQ=255)
                                                                                                                                           ttCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATg      >  5:91903/1‑149 (MQ=255)
                                                                                                                                           ttCACGGGTAAACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATg      >  7:241952/1‑149 (MQ=255)
                                                                                                                                               ctgttttACCCGCTAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGcc  <  1:172780/145‑1 (MQ=255)
                                                                                                                                               cgGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCGCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGcc  <  7:210759/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
AGCGCCCAGGTGCACGCCACGCCGTTCTATGAGCGGCTGGGCTTTCGCGTAGTCAGCGAGGAATTCCTCGAAGCTGGCATCCCGCATGTCGACATGGTGCGCGACTCGCGCGCCTGATCCAT‑CACCTGCACTGGCCTCTTCGCGGGTTTACCCGCGAAGAGGCCGGCTCAGGCGATATACATCCCCGCTGACAAAAAACCTGTACATCCATCCAGATAAAACTTGCCTCTGCGCCCCCGCTTGCGCATCCTATGGCCCACCACCCCGATGAAGCGTCCCCGGCCATGCGCC  >  NC_002947/4529878‑4530168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑