| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 432,503 | G→A | 13.3% | R33Q (CGG→CAG) | PP_0355 → | two‑component system response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 432,503 | 0 | G | A | 13.3% | 68.4 / 3.6 | 30 | R33Q (CGG→CAG) | PP_0355 | two‑component system response regulator |
| Reads supporting (aligned to +/- strand): ref base G (10/16); new base A (2/2); total (12/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.96e-01 | |||||||||||
TATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTGGCAGCCAGACCGTTGGTGCTGGTGGTGGATGACAACGCGGTTAACCGGGAG > NC_002947/432355‑432651 | tataGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCg > 8:213637/1‑149 (MQ=255) ccGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAAttttt < 4:259459/149‑1 (MQ=255) cGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAAtttttt > 8:20621/1‑149 (MQ=255) gttCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGAt < 3:270545/148‑1 (MQ=255) cttCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGAt < 8:82514/147‑1 (MQ=255) cATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAg < 7:20621/149‑1 (MQ=255) cggGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGa < 2:323801/149‑1 (MQ=255) ttGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAgcgc < 1:167879/149‑1 (MQ=255) cGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAAttttt < 1:46523/91‑1 (MQ=255) cGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAAttttt > 2:46523/1‑91 (MQ=255) aaGTCTATGAGCGCCATTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTTATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGACGCCtg > 6:287072/1‑148 (MQ=255) tATGAGCGCCCTTGTCAAAGACCCGATGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCg > 4:319746/1‑149 (MQ=255) tGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCAGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGt > 5:32661/1‑148 (MQ=255) cccTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAAc > 4:142642/1‑149 (MQ=255) gTCAAAGACCCGACGAAAGGCGAATCGGGTTACGCGAGTAATGCGGTCCTAATGGTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAg > 7:200986/1‑149 (MQ=255) gTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCATGATAAACCaaa < 3:110765/148‑1 (MQ=255) aaaGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTgc > 7:38567/1‑149 (MQ=255) aaaGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTgc < 7:213637/149‑1 (MQ=255) aaaGACCAGACGAAAGGCGAAACTGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTgc < 7:229052/149‑1 (MQ=255) aGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTgcgc < 3:142642/149‑1 (MQ=255) aCCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCAGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCt < 6:32661/149‑1 (MQ=255) ccGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCGTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCtgt < 5:287072/149‑1 (MQ=255) gacgaAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTgg > 7:70556/1‑149 (MQ=255) ggCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTGGCAGc > 3:112453/1‑146 (MQ=255) gCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTGGCAGCCAGa > 4:40569/1‑149 (MQ=255) cgcgAGTCATGCGGTCCAAAGCCTGAACCGGAAGGTTTTTGACGAGATGAGCAGGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCGGTGGCAGCCAGACCGTTGGTGCtgg < 4:279236/149‑1 (MQ=255) cgcgAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTGGCAGCCAGACCGTTGGTGCtg < 3:319746/148‑1 (MQ=255) cgcgAGTAATGCGGTCCTAATGCTGAAGCAGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTGGCAGCCAGACCGTTGGTGCtgg > 4:314429/1‑149 (MQ=255) aTGCGGTCCTAATGCTGAAGCAGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTGGCAGCCAGACCGTTGGTGCTGGTGGTGGAt < 3:314429/149‑1 (MQ=255) aGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGAGGCGGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTGGCAGCCAGACCGTTGGTGCTGGTGGTGGATGACATCGCGGTTAACCg < 8:38567/148‑1 (MQ=255) cGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTGGCAGCCAGACCGTTGGTGCTGGTGGTGGATGACAACGCGGTTAACCGGGa < 4:317131/149‑1 (MQ=255) ggAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTGGCAGCCAGACCGTTGGTGCTGGTGGTGGATGACACCGCGGTTAACCGGGAg > 2:218813/1‑149 (MQ=255) | TATAGGTACCGGCCATGTTGTTCTCTGCCTCCATGGTCGGCTTGCGGCGGGGTGGTGCCCATTGGACGTAAGTCTATGAGCGCCCTTGTCAAAGACCCGACGAAAGGCGAAACGGGTTACGCGAGTAATGCGGTCCTAATGCTGAAGCGGAATTTTTTTGACGAGATGAGCATGCCTGAGCACACCGACATCCTGAGCGACGCCGAGCGCGAGGCCTTGGCCGTGATAAACCAAAGTGCGCCTGTGGCAGCCAGACCGTTGGTGCTGGTGGTGGATGACAACGCGGTTAACCGGGAG > NC_002947/432355‑432651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |