Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,557,042 C→A 10.0% L145L (CTG→CTT)  gntZ ← 6‑phosphogluconate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,557,0420CA10.0% 119.8 / 3.6 40L145L (CTG→CTT) gntZ6‑phosphogluconate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (18/18);  new base A (2/2);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

TGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATGTCGTCC  >  NC_002947/4556894‑4557180
                                                                                                                                                    |                                                                                                                                          
tGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCCGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTcc                                                                                                                                            <  4:186112/149‑1 (MQ=255)
        tGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGAACCGCTGTTCAAGGCGCtg                                                                                                                                    <  7:213917/149‑2 (MQ=255)
           aCCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGAGCCGCTGTTCAAGgcg                                                                                                                                       <  3:259630/143‑1 (MQ=255)
           aCCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTGGACCCGCTGTTCAAGgcg                                                                                                                                       >  4:259630/1‑143 (MQ=255)
               ttttCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACg                                                                                                                             <  6:48268/149‑1 (MQ=255)
                    aCGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCtt                                                                                                                        >  2:101015/1‑149 (MQ=255)
                    aCGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCtt                                                                                                                        >  5:279432/1‑149 (MQ=255)
                                    ccGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGatcat                                                                                                         >  5:272959/1‑148 (MQ=255)
                                        tGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGc                                                                                                    <  8:39011/149‑1 (MQ=255)
                                               gCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGcc                                                                                             >  7:305307/1‑149 (MQ=255)
                                                 aTGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCcgc                                                                                           >  1:309855/1‑149 (MQ=255)
                                                     aTGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTc                                                                                                                     >  8:293375/1‑119 (MQ=255)
                                                     aTGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTc                                                                                                                     <  7:293375/119‑1 (MQ=255)
                                                        tAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCa                                                                                     <  5:121524/148‑1 (MQ=255)
                                                        tAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCa                                                                                     <  6:272959/148‑1 (MQ=255)
                                                                 tCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCcgcg                                                                                          <  6:55878/134‑1 (MQ=255)
                                                                 tCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCcgcg                                                                                          >  5:55878/1‑134 (MQ=255)
                                                                        gCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGccgccg                                                                    >  8:145015/1‑149 (MQ=255)
                                                                           ggTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGaa                                                                 <  6:279432/149‑1 (MQ=255)
                                                                            gTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGaa                                                                 <  7:145015/148‑1 (MQ=255)
                                                                                tCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGaa                                                                 >  1:297303/1‑144 (MQ=255)
                                                                                tCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGaa                                                                 <  2:297303/144‑1 (MQ=255)
                                                                                            ccATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGAt                                                 >  1:257877/1‑148 (MQ=255)
                                                                                                gggTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTc                                                              <  5:169482/131‑1 (MQ=255)
                                                                                                gggTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGACGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTc                                                              >  6:169482/1‑131 (MQ=255)
                                                                                                  gTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAg                                          <  3:83727/149‑1 (MQ=255)
                                                                                                        gggATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCcgcg                                    >  4:273411/1‑149 (MQ=255)
                                                                                                           aTGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCtt                                 <  4:251346/149‑1 (MQ=255)
                                                                                                              tCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGc                              <  2:309855/149‑1 (MQ=255)
                                                                                                                gCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGCCGTCCAGATAATGCAGGCCGCGCTGGGc                              >  6:285076/1‑147 (MQ=255)
                                                                                                                 cccACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGc                              <  5:248475/146‑1 (MQ=255)
                                                                                                                 cccACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGc                              >  6:248475/1‑146 (MQ=255)
                                                                                                                  ccACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGt                          >  6:174173/1‑149 (MQ=255)
                                                                                                                    aCGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGTTCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTc                        >  8:227818/1‑149 (MQ=255)
                                                                                                                      gCCCGGCGCCAGCGCCTTGACCAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTg                      <  6:277921/149‑1 (MQ=255)
                                                                                                                        ccGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCTCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCa                    <  8:305307/149‑1 (MQ=255)
                                                                                                                        ccGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTg                      >  3:17077/1‑147 (MQ=255)
                                                                                                                                 gcgcCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTgg                               <  3:117564/129‑1 (MQ=255)
                                                                                                                                 gcgcCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTgg                               >  4:117564/1‑129 (MQ=255)
                                                                                                                                   gcCTGGAACCGCTGTTCAAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCAt         >  5:175653/1‑149 (MQ=255)
                                                                                                                                          aCAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGTTCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATGTCGTcc  <  7:227818/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
TGCCGTTATGCACCATTTTCACGTAGTGCCCGGCGCCCGGTGGACCGGCATGGATGTAGCCGTGCTCGGCACGCTGGTGCTCGCCGCTGCGGCCATGGGTACGCGGGATGTCGCCCACGCCCGGCGCCAGCGCCTTGAACAGCGGTTCCAGGCGCTCGAACACGTCCTTTTCGCCGCCGATCATCATGCAGTAGCCGCGGTCCAGGCCCCACACGCCGCCGGAAGTCCCGACGTCCAGATAATGCAGGCCGCGCTTGGCCAGTTCTGCAGCGCGGCGCATGTCGTCC  >  NC_002947/4556894‑4557180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑