Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,612,655 A→T 7.3% intergenic (+1617/‑490) hcpC‑II → / → PP_4084 hemolysin‑coregulated protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,612,6550AT 7.3% 164.6 / 2.7 55intergenic (+1617/‑490)hcpC‑II/PP_4084hemolysin‑coregulated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (23/28);  new base T (2/2);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

ACCAGCCGATGATCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGCGTC  >  NC_002947/4612508‑4612794
                                                                                                                                                   |                                                                                                                                            
aCCAGCCGATGATCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAag                                                                                                                                             <  4:55401/149‑1 (MQ=255)
      cGATGATCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGaa                                                                                                                                       <  1:288041/149‑1 (MQ=255)
        atgatCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATTTTGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAg‑t                                                                                                                                    <  1:267789/149‑1 (MQ=255)
          gatCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAg‑tg                                                                                                                                   <  3:907/148‑1 (MQ=255)
           atCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAg‑tgt                                                                                                                                  <  4:86415/148‑1 (MQ=255)
               ccGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGAAAGGAGGAAG‑TGTTCAt                                                                                                                              >  3:242276/1‑148 (MQ=255)
                     gcACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGcc                                                                                                                       <  6:233586/149‑1 (MQ=255)
                       aCTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGcc                                                                                                                       >  2:49586/1‑147 (MQ=255)
                         ttACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCa                                                                                                                     >  7:133715/1‑147 (MQ=255)
                           aCTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCATAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCaga                                                                                                                   <  1:194706/147‑1 (MQ=255)
                           aCTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCaga                                                                                                                   <  7:229001/147‑1 (MQ=255)
                            cTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCaga                                                                                                                   >  2:145944/1‑146 (MQ=255)
                               cGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCATGGATGAAC‑ACTTCCTCCTTGCCCagctcat                                                                                                               <  2:17230/147‑6 (MQ=21)
                                  tGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAg                                                                                                           >  4:149101/1‑148 (MQ=255)
                                         cTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACActct                                                                                                   >  2:94686/1‑149 (MQ=255)
                                         cTGCCGCCGTTCGATCTGCCCGATCACAAAACACGCATTACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACActct                                                                                                   <  8:133715/149‑1 (MQ=255)
                                            ccgccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAGCTGTTCATCCATGCCCAGAGAGATCAGAACACTCTg                                                                                                  <  1:49586/148‑1 (MQ=255)
                                            ccgccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTg                                                                                                  <  6:261261/147‑1 (MQ=255)
                                            ccgccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATACCCAGAGAGATCAGAACACTCTg                                                                                                  <  7:509/147‑1 (MQ=255)
                                             cgccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGt                                                                                                >  4:22310/1‑148 (MQ=255)
                                             cgccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGt                                                                                                <  3:22310/148‑1 (MQ=255)
                                               ccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCa                                                                                              >  1:157724/1‑148 (MQ=255)
                                               ccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCa                                                                                              <  2:157724/148‑1 (MQ=255)
                                                     gATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACaa                                                                                       >  1:165324/1‑149 (MQ=255)
                                                         tGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAg                                                                                   >  7:302415/1‑149 (MQ=255)
                                                          gCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAAt                                                                                            <  7:128898/139‑1 (MQ=255)
                                                          gCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAAt                                                                                            >  8:128898/1‑139 (MQ=255)
                                                           ccagATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACaa                                                                                       <  1:145944/140‑1 (MQ=255)
                                                                tCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCctct                                                                            <  2:165324/149‑1 (MQ=255)
                                                                  acaAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCctctct                                                                          >  2:324107/1‑149 (MQ=255)
                                                                   caAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAAc                                                                                      <  7:262539/136‑1 (MQ=255)
                                                                   caAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAAc                                                                                      >  8:262539/1‑136 (MQ=255)
                                                                    aaaaCACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCtgt                                                                        >  6:13597/1‑149 (MQ=255)
                                                                        cacGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGc                                                                    <  1:324107/149‑1 (MQ=255)
                                                                         acGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGt                                                                                                >  2:96184/1‑120 (MQ=255)
                                                                         acGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGt                                                                                                <  1:96184/120‑1 (MQ=255)
                                                                                  cATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCa                                                          <  5:13597/149‑1 (MQ=255)
                                                                                    tCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCAcc                                                        >  4:83383/1‑149 (MQ=255)
                                                                                       agagCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCaa                                                     <  4:242276/149‑1 (MQ=255)
                                                                                           ccAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCagaga                                                                                                                 <  5:126850/85‑1 (MQ=255)
                                                                                           ccAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCagaga                                                                                                                 >  6:126850/1‑85 (MQ=255)
                                                                                             aGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGtt                                               <  3:149101/149‑1 (MQ=255)
                                                                                                   acaAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAg                                         >  4:288744/1‑149 (MQ=255)
                                                                                                          ggAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCTCCCAACAGGTTGGCCAGGACGAAt                                  <  8:302415/149‑1 (MQ=255)
                                                                                                                     aCGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCAtt                       >  3:159026/1‑149 (MQ=255)
                                                                                                                       gAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTgg                     <  3:288744/149‑1 (MQ=255)
                                                                                                                           tGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCg                  >  8:15737/1‑148 (MQ=255)
                                                                                                                                 tCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCAtt                       >  6:335827/1‑137 (MQ=255)
                                                                                                                                 tCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCAtt                       <  5:335827/137‑1 (MQ=255)
                                                                                                                                  cGAGGATGAGCTGGGCAAGGAGGAAG‑TGCTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACCACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGt           <  6:192024/148‑1 (MQ=255)
                                                                                                                                       tctctcTGGGCATGGATGAAC‑ACTTCCTCCTTGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGAc                                      <  7:110922/111‑1 (MQ=25)
                                                                                                                                       tctctcTGGGCATGGATGAAC‑ACTTCCTCCTTGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGAc                                      >  8:110922/6‑116 (MQ=25)
                                                                                                                                         gAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGcgt   >  7:3703/1‑149 (MQ=255)
                                                                                                                                          ctcTGGGCATGGATGAAC‑ACTTCCTCCTTGCCCAGAGTGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGcgtc  >  1:58300/3‑149 (MQ=38)
                                                                                                                                           gCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCTGGACGAATCGGTCGCCATTGGCCGCAATCGTCTgcgc     >  5:282077/1‑145 (MQ=255)
                                                                                                                                           gCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTgcgc     <  6:282077/145‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                            
ACCAGCCGATGATCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGCGTC  >  NC_002947/4612508‑4612794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑