| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,612,664 | T→A | 7.4% | intergenic (+1626/‑481) | hcpC‑II → / → PP_4084 | hemolysin‑coregulated protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,612,664 | 0 | T | A | 7.4% | 164.0 / 3.0 | 54 | intergenic (+1626/‑481) | hcpC‑II/PP_4084 | hemolysin‑coregulated protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (24/26); new base A (2/2); total (26/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.99e-01 | |||||||||||
ATGATCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGCGTCGTCAAGGCCAACGACAC > NC_002947/4612516‑4612811 | atgatCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATTTTGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAg‑t < 1:267789/149‑1 (MQ=255) gatCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAg‑tg < 3:907/148‑1 (MQ=255) atCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAg‑tgt < 4:86415/148‑1 (MQ=255) ccGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGAAAGGAGGAAG‑TGTTCAt > 3:242276/1‑148 (MQ=255) gcACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGcc < 6:233586/149‑1 (MQ=255) aCTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGcc > 2:49586/1‑147 (MQ=255) ttACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCa > 7:133715/1‑147 (MQ=255) aCTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCATAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCaga < 1:194706/147‑1 (MQ=255) aCTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCaga < 7:229001/147‑1 (MQ=255) cTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCaga > 2:145944/1‑146 (MQ=255) cGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCATGGATGAAC‑ACTTCCTCCTTGCCCagctcat < 2:17230/147‑6 (MQ=21) tGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAg > 4:149101/1‑148 (MQ=255) cTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACActct > 2:94686/1‑149 (MQ=255) cTGCCGCCGTTCGATCTGCCCGATCACAAAACACGCATTACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACActct < 8:133715/149‑1 (MQ=255) ccgccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAGCTGTTCATCCATGCCCAGAGAGATCAGAACACTCTg < 1:49586/148‑1 (MQ=255) ccgccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTg < 6:261261/147‑1 (MQ=255) ccgccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATACCCAGAGAGATCAGAACACTCTg < 7:509/147‑1 (MQ=255) cgccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGt > 4:22310/1‑148 (MQ=255) cgccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGt < 3:22310/148‑1 (MQ=255) ccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCa > 1:157724/1‑148 (MQ=255) ccTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCa < 2:157724/148‑1 (MQ=255) gATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACaa > 1:165324/1‑149 (MQ=255) tGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAg > 7:302415/1‑149 (MQ=255) gCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAAt < 7:128898/139‑1 (MQ=255) gCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAAt > 8:128898/1‑139 (MQ=255) ccagATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACaa < 1:145944/140‑1 (MQ=255) tCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCctct < 2:165324/149‑1 (MQ=255) acaAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCctctct > 2:324107/1‑149 (MQ=255) caAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAAc > 8:262539/1‑136 (MQ=255) caAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAAc < 7:262539/136‑1 (MQ=255) aaaaCACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCtgt > 6:13597/1‑149 (MQ=255) cacGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGc < 1:324107/149‑1 (MQ=255) acGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGt > 2:96184/1‑120 (MQ=255) acGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGt < 1:96184/120‑1 (MQ=255) cATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCa < 5:13597/149‑1 (MQ=255) tCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCAcc > 4:83383/1‑149 (MQ=255) agagCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCaa < 4:242276/149‑1 (MQ=255) ccAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCagaga < 5:126850/85‑1 (MQ=255) ccAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCagaga > 6:126850/1‑85 (MQ=255) aGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGtt < 3:149101/149‑1 (MQ=255) acaAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAg > 4:288744/1‑149 (MQ=255) ggAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCTCCCAACAGGTTGGCCAGGACGAAt < 8:302415/149‑1 (MQ=255) aCGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCAtt > 3:159026/1‑149 (MQ=255) gAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTgg < 3:288744/149‑1 (MQ=255) tGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCg > 8:15737/1‑148 (MQ=255) tCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCAtt > 6:335827/1‑137 (MQ=255) tCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCAtt < 5:335827/137‑1 (MQ=255) cGAGGATGAGCTGGGCAAGGAGGAAG‑TGCTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACCACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGt < 6:192024/148‑1 (MQ=255) tctctcTGGGCATGGATGAAC‑ACTTCCTCCTTGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGAc < 7:110922/111‑1 (MQ=25) tctctcTGGGCATGGATGAAC‑ACTTCCTCCTTGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGAc > 8:110922/6‑116 (MQ=25) gAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGcgt > 7:3703/1‑149 (MQ=255) ctcTGGGCATGGATGAAC‑ACTTCCTCCTTGCCCAGAGTGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGcgtc > 1:58300/3‑149 (MQ=38) gCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCTGGACGAATCGGTCGCCATTGGCCGCAATCGTCTgcgc > 5:282077/1‑145 (MQ=255) gCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTgcgc < 6:282077/145‑1 (MQ=255) ggaggaAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGCGTCGTCaa < 6:295193/144‑1 (MQ=255) ggaggaAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGCGTCGTCaa > 5:295193/1‑144 (MQ=255) aggaAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGCGTCGTCAAGGCCAAc < 3:83383/149‑1 (MQ=255) g‑tgtTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGCGTCGTCAAGGCCAACGacac < 8:3703/149‑1 (MQ=255) | ATGATCACCGGGCGCACTTACTGCGGTGACCAACTGCCGCCTTACGATCTGCCCGATCACAAAACACGCATGACCATCAAGAGCCAGACCCACAAGGGGGAAGGTTTCAACGAGCTGCGTTTCGAGGATGAGCTGGGCAAGGAGGAAG‑TGTTCATCCATGCCCAGAGAGATCAGAACACTCTGGTCAATCACAACCAGTCCCTCTCTGTGGGCGTCGATCGCACCCAACAGGTTGGCCAGGACGAATCGGTCGCCATTGGCCGCAATCGTCTGCGCGTCGTCAAGGCCAACGACAC > NC_002947/4612516‑4612811 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |