Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,694,425	C→G	5.9%	P767R (CCC→CGC)	PP_4154 →	class 3 aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,694,425	0	C	G	5.9%	220.4 / 3.0	68	P767R (CCC→CGC)	PP_4154	class 3 aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (33/31);  new base G (2/2);  total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

TTCCGCGGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAC  >  NC_002947/4694277‑4694569                                                                                                                                                      |                                                                                                                                                 ttCCGCGGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGcc                                                                                                                                                  >  8:283330/1‑149 (MQ=255) ttCCGCGGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGcc                                                                                                                                                  >  8:15698/1‑149 (MQ=255)                     ggCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAg                                                                                                                              <  7:161287/149‑1 (MQ=255)                     ggCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCGCGCAGGTAGCCTGCGGGCAg                                                                                                                              <  1:303347/149‑1 (MQ=255)                        cGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGcc                                                                                                                            >  2:173170/1‑148 (MQ=255)                          aCAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTAt                                                                                                                         <  4:188699/149‑1 (MQ=255)                                ccgccgACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAg                                                                                                                   <  5:68602/149‑1 (MQ=255)                                 cgccgACTACCTGCAAGAAGTGGACGCCAATCTGGCTGACCTCGACGCCCGTGGCCGTGAGCGGGCGGGCATCATCTGCGAACCGGTGTACGGCAATGCCCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAg                                                                                                                   <  1:77542/149‑1 (MQ=255)                                    cgACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTcc                                                                                                               <  8:169793/149‑1 (MQ=255)                                    cgACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTcc                                                                                                               <  7:283330/149‑1 (MQ=255)                                    cgACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTcc                                                                                                               <  8:257649/149‑1 (MQ=255)                                    cgACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTcc                                                                                                               <  7:15698/149‑1 (MQ=255)                                        tACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCgcgc                                                                                                           >  8:24891/1‑149 (MQ=255)                                          ccTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGccc                                                                                                         >  6:200131/1‑149 (MQ=255)                                           cTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCcg                                                                                                        >  6:31111/1‑149 (MQ=255)                                             gCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCcgcg                                                                                                      >  1:286483/1‑149 (MQ=255)                                             gCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCcgc                                                                                                       <  1:109304/148‑1 (MQ=255)                                             gCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCcgc                                                                                                       >  2:109304/1‑148 (MQ=255)                                             gCAAGACGTCGACGCCAAGCTCGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCcgcg                                                                                                      >  3:179721/1‑149 (MQ=255)                                                aGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCgcggcg                                                                                                   >  4:204397/1‑149 (MQ=255)                                                    gTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                               <  4:149647/149‑1 (MQ=255)                                                    gTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                               <  3:265727/149‑1 (MQ=255)                                                       gACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                            >  5:312193/1‑149 (MQ=255)                                                       gACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                            >  3:33092/1‑149 (MQ=255)                                                        aCGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGtgtg                                                                                            >  1:81296/1‑148 (MQ=255)                                                           ccAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATc                                                                                        >  4:193491/1‑149 (MQ=255)                                                            cAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATg                                                                                                                        >  8:190105/1‑116 (MQ=255)                                                             aaGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGc                                                                                      <  3:193491/149‑1 (MQ=255)                                                              aGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGcc                                                                                     <  3:182032/149‑1 (MQ=255)                                                              aGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGcc                                                                                     >  1:306073/1‑149 (MQ=255)                                                                 tggctgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCcgac                                                                                  >  4:164760/1‑149 (MQ=255)                                                                     tgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                               <  1:11098/132‑1 (MQ=255)                                                                     tgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgt                                                                                               >  2:11098/1‑132 (MQ=255)                                                                     tgACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAg                                                                              >  6:73190/1‑149 (MQ=255)                                                                       aCCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAg                                                                              <  3:5790/147‑1 (MQ=255)                                                                       aCCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGt                                                                             <  5:73190/148‑1 (MQ=255)                                                                       aCCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTg                                                                            >  2:290290/1‑149 (MQ=255)                                                                        ccTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTg                                                                            >  4:112189/1‑148 (MQ=255)                                                                         cTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGaa                                                                               >  4:261292/1‑144 (MQ=255)                                                                         cTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTg                                                                            >  5:146708/1‑147 (MQ=255)                                                                         cTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTg                                                                            >  8:120147/1‑147 (MQ=255)                                                                         cTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTg                                                                            >  2:209355/1‑147 (MQ=255)                                                                         cTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTg                                                                            >  7:137533/1‑147 (MQ=255)                                                                         cTCGACGCCCGTGGCCGTCAGCTGGCCGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGaa                                                                               <  3:261292/144‑1 (MQ=255)                                                                                 ccGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgtgt                                                                                             <  8:262307/122‑1 (MQ=255)                                                                                 ccGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGgtgt                                                                                             >  7:262307/1‑122 (MQ=255)                                                                                       ccGTCAGCTGGCGGGCATCATATGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGgcc                                                            >  2:305143/1‑149 (MQ=255)                                                                                           cAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCt                                                        >  7:187365/1‑149 (MQ=255)                                                                                            aGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTg                                                       >  3:172730/1‑149 (MQ=255)                                                                                               tGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGc                                                    <  2:306073/149‑1 (MQ=255)                                                                                                 gCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGc                                                    <  4:245704/147‑1 (MQ=255)                                                                                                 gCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGc                                                    <  6:312193/147‑1 (MQ=255)                                                                                                       atcatcTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACtt                                            >  2:322183/1‑149 (MQ=255)                                                                                                               cGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTgg                                        <  5:200131/145‑1 (MQ=255)                                                                                                               cGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTgg                                        <  3:164760/145‑1 (MQ=255)                                                                                                                   ccGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGa                                <  7:120147/149‑1 (MQ=255)                                                                                                                   ccGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGa                                <  2:286483/149‑1 (MQ=255)                                                                                                                      gtgtACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACtt                                            >  8:92491/1‑134 (MQ=255)                                                                                                                      gtgtACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACtt                                            <  7:92491/134‑1 (MQ=255)                                                                                                                           cGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAgg                        <  5:31111/149‑1 (MQ=255)                                                                                                                           cGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAg                         <  4:226790/148‑1 (MQ=255)                                                                                                                               aaTG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCgt                    >  4:272450/1‑149 (MQ=255)                                                                                                                                    ccGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCgtggt                 <  1:305143/148‑1 (MQ=255)                                                                                                                                    ccGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCgtggt                 <  1:173170/148‑1 (MQ=255)                                                                                                                                           tATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGAca         <  3:272450/149‑1 (MQ=255)                                                                                                                                               tcGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGATATGGCCGCATGGGCGAGTAGTGGTGGGGGTACGAGGACCAGGGGGTGGTCCGGGAcagcat     >  4:36897/1‑149 (MQ=255)                                                                                                                                                   tGCGCGCAGGTAGCCTGCGGGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc  >  7:173148/1‑148 (MQ=255)                                                                                                                                                   tGCGCGCAGGTAGCCTGCGGGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAc  >  1:101559/1‑148 (MQ=255)                                                                                                                                                   tGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCgtgg                  >  2:24374/1‑132 (MQ=255)                                                                                                                                                   tGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCgtgg                  <  1:24374/132‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                 TTCCGCGGCCGCTTCCGCGGGGCCGACAGCGCCGCCGACTACCTGCAAGACGTCGACGCCAAGCTGGCTGACCTCGACGCCCGTGGCCGTCAGCTGGCGGGCATCATCTGCGAACCGGTGTACGGCAATG‑CCGGCGGTATCTCGCTGCCCGCAGGCTACCTGCGCGCAGCCTATGCCAAGGTCCGCGCCCGCGGCGGGGTGTGCATCGCCGACGAAGTGCAGGTAGGCTATGGCCGCCTGGGCGAGTACTTCTGGGGCTTCGAGGAACAGGGCGTGGTGCCGGACATCATTAC  >  NC_002947/4694277‑4694569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑