| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,718,688 | C→A | 10.1% | intergenic (+32/+99) | fabB → / ← PP_4176 | 3‑oxoacyl‑ACP synthase/5‑oxo‑L‑prolinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,718,688 | 0 | C | A | 10.1% | 173.3 / 9.2 | 60 | intergenic (+32/+99) | fabB/PP_4176 | 3‑oxoacyl‑ACP synthase/5‑oxo‑L‑prolinase |
| Reads supporting (aligned to +/- strand): ref base C (25/27); new base A (2/4); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
TTGCCAATCCTGCGTAAAACCGAAGAAAACGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGGCGCGCCTTGGGCAACACCGCCAGAAAATTCTCCCGG > NC_002947/4718540‑4718829 | ttGCCAATCCTGCGTAAAACCGAAGAAAACGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGAc > 4:316476/1‑149 (MQ=255) tCCTGCGTAAAACCGAAGAAAACGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCg < 2:197670/149‑1 (MQ=255) cTGCGTAAAACCGAAGAAAACGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCggg > 2:11324/1‑149 (MQ=255) cTGCGTAAAACCGAAGAAAACGCCAAGATCGACACGATCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCggg < 4:49719/149‑1 (MQ=255) aaaCCGAAGAAAACGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGttt < 7:165670/148‑1 (MQ=255) aaCCGAAGAAAACGCCAAGATCGACACGGTCATGAGCAACATCTTCGGCTTCTGCGGCACCAACGCCACCCTGGTGTTCAAGCGC‑TTGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGAACCAGTCGGGGCGttttc < 7:129771/149‑2 (MQ=255) ccGAAGAAAACGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGAACCAGTCGGGGCGttttcat < 6:315982/149‑4 (MQ=255) gaagaaAACGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGAACCAGTCGGGGCGtt > 4:302848/1‑142 (MQ=255) gaagaaAACGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGAACCAGTCGGGGCGtt < 3:302848/142‑1 (MQ=255) aagaaAACGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTtttgtttg < 4:297783/149‑1 (MQ=255) aaaaCGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGt > 5:266538/1‑149 (MQ=255) aaaCGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGtt > 5:224007/1‑149 (MQ=255) ccGCCAAGAGCGCCACGGTCATCAGCAACAGCTTCGGCTTCGGCTGCACCAACGCCTCCGTGGTGCTCAAGCGC‑TGGGAAGGCTAGTAAGACGCTGCTGATGTAAATGATATCGCGACGAGTGGTTCGGGGAGTTTTTGTTTGGGTTGc < 4:242600/148‑1 (MQ=255) gCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCt > 3:299304/1‑149 (MQ=255) cAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCtgt > 2:260976/1‑149 (MQ=255) cAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCtgt > 6:37038/1‑149 (MQ=255) aaGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCATGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCtgtg > 4:37914/1‑149 (MQ=255) gATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGAACCAGTCGGGGCGtt < 2:169302/128‑1 (MQ=255) gATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGAACCAGTCGGGGCGtt > 1:169302/1‑128 (MQ=255) tCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTcgc > 7:58705/1‑149 (MQ=255) aGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGt < 3:316476/148‑1 (MQ=255) gCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGCGTGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGt > 1:247516/1‑148 (MQ=255) gcttcggcttcgGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGc < 5:17835/149‑1 (MQ=255) ggcttcggcGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTa < 5:138467/149‑1 (MQ=255) gcttcggcGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTac < 2:247516/149‑1 (MQ=255) ttcggcGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTacac < 8:58705/149‑1 (MQ=255) cggcggCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTacac < 5:37038/147‑1 (MQ=255) gcggcACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGt < 4:299304/149‑1 (MQ=255) aCCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCATGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCAttt < 3:37914/149‑1 (MQ=255) aCCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCAttt < 1:11324/149‑1 (MQ=255) cAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGCTTCGGGGCGATTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCAtttc > 1:239640/1‑147 (MQ=255) cccTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTtg < 7:286955/149‑1 (MQ=255) ccTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTAcaca < 4:235384/130‑1 (MQ=255) ccTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTAcaca > 3:235384/1‑130 (MQ=255) tCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGAGTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTg > 6:95978/1‑149 (MQ=255) aaGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTg > 2:42169/1‑147 (MQ=255) aaGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCa < 6:15032/149‑1 (MQ=255) aGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAg < 6:266538/149‑1 (MQ=255) gc‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGc > 5:180089/1‑148 (MQ=255) aaGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCgg < 1:300953/86‑1 (MQ=255) aaGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCgg > 2:300953/1‑86 (MQ=255) aaGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGGCGCGcc > 3:220624/1‑149 (MQ=255) gTAAGACGCTGCTGCTGTAAATGAATACGGCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGGCGCGCCtt < 4:51812/149‑1 (MQ=255) tAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGc < 6:74592/93‑1 (MQ=255) tAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGc > 5:74592/1‑93 (MQ=255) aGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGGCGCGCCTTgg < 1:260976/148‑1 (MQ=255) ctgTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAAcc < 8:4154/77‑1 (MQ=255) ctgTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAAcc > 7:4154/1‑77 (MQ=255) ctgTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGGCGCGCCTTGGGCAACACCGc > 7:13979/1‑147 (MQ=255) tgTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTtgt > 2:157845/1‑107 (MQ=255) tgTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTtgt < 1:157845/107‑1 (MQ=255) gTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCCCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGTCGCGCCTTGGGCAACACCGCCAg > 5:164065/1‑148 (MQ=255) aaaTGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTgg > 5:37252/1‑106 (MQ=255) aaaTGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTgg < 6:37252/106‑1 (MQ=255) aaTGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGGCGCGCCTTGGGCAACACCGCCAGaaaa > 4:2317/1‑149 (MQ=255) aaTGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGGCGCGCCTTGGGCAACACCGCCAGaaaa > 6:30946/1‑149 (MQ=255) aaTGAAAACGCCCCGACTGGTTC‑GGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGAGCTTGTGGCCATTGCAGGCTAATGGCGCGCCTTGGGCAACACCGCCAGAAAAt > 5:68625/1‑149 (MQ=255) aaaaCGCCCCGACTGGTTCGGGGGGTTTTTGTTTGGGTTGCCTGTGCTGGGCTCTTCGCGGGTATACCCGCTCCTACACAGGTGATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGGCGCGCCTTGGGCAACACCGCCAGAAAATtc < 2:239640/148‑1 (MQ=255) gCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGGCGCGCCTTGGGCAACACCGCCAGAAAATTCTCCCgg < 3:2317/149‑1 (MQ=255) cccGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGc > 1:250395/1‑64 (MQ=255) cccGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGc < 2:250395/64‑1 (MQ=255) | TTGCCAATCCTGCGTAAAACCGAAGAAAACGCCAAGATCGACACGGTCATGAGCAACAGCTTCGGCTTCGGCGGCACCAACGCCACCCTGGTGCTCAAGCGC‑TGGGAAGGCAAGTAAGACGCTGCTGCTGTAAATGAAAACGCCCCGACTGGTTCGGGGCGTTTTTGTTTGGGTTGCCTGTGCTGGCCTCTTCGCGGGTAAACCCGCTCCTACACAGGTCATTTGCAGTGCTTGTGGCCATTGCAGGCTAATGGCGCGCCTTGGGCAACACCGCCAGAAAATTCTCCCGG > NC_002947/4718540‑4718829 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |