Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 483,678 A→G 8.1% L254P (CTG→CCG)  yeaG ← protein kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947483,6780AG 8.1% 135.5 / 2.4 49L254P (CTG→CCG) yeaGprotein kinase
Reads supporting (aligned to +/- strand):  ref base A (20/25);  new base G (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

CTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTCATCGCCGGGTTCGGTCTTGGCAAT  >  NC_002947/483530‑483826
                                                                                                                                                    |                                                                                                                                                    
cTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCa                                                                                                                                                      <  2:255032/149‑1 (MQ=255)
   cGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAgg                                                                                                                                                    <  1:246048/148‑1 (MQ=255)
     aTTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGccc                                                                                                                                                 <  4:286339/149‑1 (MQ=255)
        cGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTg                                                                                                                                               <  2:325546/148‑1 (MQ=255)
         gTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGt                                                                                                                                             >  6:199099/1‑149 (MQ=255)
                     cagcagAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCaaa                                                                                                                                 <  8:16697/149‑1 (MQ=255)
                     cagcagAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTACAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCaaa                                                                                                                                 <  1:263949/149‑1 (MQ=255)
                          gAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAgcgcg                                                                                                                            >  1:44441/1‑149 (MQ=255)
                                  gTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTa                                                                                                                    <  3:266694/149‑1 (MQ=255)
                                    ataGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGc                                                                                                                  >  2:18607/1‑149 (MQ=255)
                                        gggATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAg                                                                                                                   <  8:79929/144‑1 (MQ=255)
                                        gggATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAg                                                                                                                   >  7:79929/1‑144 (MQ=255)
                                         ggATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAg                                                                                                             <  2:44441/149‑1 (MQ=255)
                                           aTCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTTAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAggc                                                                                                           <  1:262022/149‑1 (MQ=255)
                                               ccccAAGGGCTTCGGTACTTTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAggcgtcg                                                                                                       <  6:282653/149‑1 (MQ=255)
                                                  cAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAggcgtcggcg                                                                                                    >  7:271250/1‑149 (MQ=255)
                                                             gTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTgg                                                                                         <  5:292705/149‑1 (MQ=255)
                                                              tACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTgg                                                                                         <  1:262070/148‑1 (MQ=255)
                                                              tACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTgg                                                                                         >  2:262070/1‑148 (MQ=255)
                                                                     gtAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTgg                                                                                         <  3:259208/141‑1 (MQ=255)
                                                                     gtAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTgg                                                                                         >  4:259208/1‑141 (MQ=255)
                                                                      tAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCt                                                                                 >  4:197609/1‑148 (MQ=255)
                                                                           gCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGtt                                                                           >  5:291189/1‑149 (MQ=255)
                                                                              ttCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTc                                                                                   <  2:59885/138‑1 (MQ=255)
                                                                              ttCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTc                                                                                   >  1:59885/1‑138 (MQ=255)
                                                                                ccTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGc                                                                        <  6:291189/147‑1 (MQ=255)
                                                                                      tGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGtt                                                                                               >  3:216000/1‑118 (MQ=255)
                                                                                      tGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATACGCGTCGGCGTCGtt                                                                                               <  4:216000/118‑1 (MQ=255)
                                                                                        tcGGTTATCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTTGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGAc                                                              <  7:274247/148‑1 (MQ=255)
                                                                                          ggTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGCCCt                                                            >  4:86649/1‑149 (MQ=255)
                                                                                                         cAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCg                                             <  1:18607/149‑1 (MQ=255)
                                                                                                            gACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAggcgtcggcgt                                                                                                   <  4:281624/92‑1 (MQ=255)
                                                                                                            gACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAggcgtcggcgt                                                                                                   >  3:281624/1‑92 (MQ=255)
                                                                                                               cTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCtt                                                           >  3:161784/1‑129 (MQ=255)
                                                                                                               cTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCtt                                                           <  4:161784/129‑1 (MQ=255)
                                                                                                                ttGATCGGCGCCTTGAACATTTCGACGAATTCCATCGGGCCCTGGTTGTCCCGGCAAAGCGCGCCCGAGTAGCTATATGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGc                                                                        <  8:87120/115‑1 (MQ=255)
                                                                                                                ttGATCGGCGCCTTGAACATTTCGACGAATTCCATCGGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGc                                                                        >  7:87120/1‑115 (MQ=255)
                                                                                                                       gcgcCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGgttg                               >  8:107095/1‑149 (MQ=255)
                                                                                                                          ccTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGttt                            <  8:271250/149‑1 (MQ=255)
                                                                                                                              gAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCGGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTc                          <  1:268258/147‑1 (MQ=255)
                                                                                                                               aaCATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCtat                                                                                                               >  5:155486/1‑61 (MQ=255)
                                                                                                                               aaCATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCtat                                                                                                               <  6:155486/61‑1 (MQ=255)
                                                                                                                               aaCATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTCATc                       >  3:176793/1‑149 (MQ=255)
                                                                                                                                   tttCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTCATCGCCg                   >  8:197327/1‑149 (MQ=255)
                                                                                                                                       gacgaATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTCATCGCCGGGtt               >  7:86156/1‑149 (MQ=255)
                                                                                                                                             ttCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTCATCGCCGGGTTCg             >  7:39756/1‑145 (MQ=255)
                                                                                                                                             ttCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTCATCGCCGGGTTCg             <  8:39756/145‑1 (MQ=255)
                                                                                                                                                cATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTCATCGCCGGGTTCGGTCTTg       <  4:176793/148‑1 (MQ=255)
                                                                                                                                                 aTCGGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAgg                                                 >  6:143815/1‑105 (MQ=255)
                                                                                                                                                 aTCGGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAgg                                                 <  5:143815/105‑1 (MQ=255)
                                                                                                                                                    aGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTCATCGCCGGGTTCGGTCTTGGCaa   <  7:197327/148‑1 (MQ=255)
                                                                                                                                                    aGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTCATCGCCGGGTTCGGTCTTGGCAAt  <  5:199099/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CTCCGATTCGTTGGAGTGCGCCAGCAGAATGCCGGTATAGGGGATCGCCCCAAGGCCTTCGGTACTGTTGTAGTTGCCTTCCTGGGTGGCGGTGAGCAAGGGGTGCAGGACCTTGATCGGCGCCTTGAACATTTCGACGAATTCCATCAGGCCCTGGTTGGCCCGGCAAAGCGCGCCCGAGTAGCTATAGGCGTCGGCGTCGTTCTGTGGGTATTCCTCAAGTTTGCGGATATCGACCTTGCCGACCAGGGCCGAGATGTCCTGGTTGTTTTCATCGCCGGGTTCGGTCTTGGCAAT  >  NC_002947/483530‑483826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑