Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,759,514	G→A	100%	G90G (GGC→GGT)	pvdP ←	pyoverdine biosynthesis‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,759,514	0	G	A	100.0%	152.9 / NA	51	G90G (GGC→GGT)	pvdP	pyoverdine biosynthesis‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/24);  total (27/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCGCCATCGGCACTGTCGAACAAGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGGCCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCTGGGTCGCCGTATCGGCCAAT  >  NC_002947/4759376‑4759652                                                                                                                                            |                                                                                                                                           gccgccATCGGCACTGTCGAACAAGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTgcg                                                                                                                                  >  2:252725/1‑149 (MQ=255)  ccgccATCGGCACTGTCGAACAAGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTgcg                                                                                                                                  >  4:220010/1‑148 (MQ=255)   cgccATCGGCACTGTCGAACAAGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTgcgc                                                                                                                                 >  5:215616/1‑148 (MQ=255)    gccATCGGCACTGTCGAACAAGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCaa                                                                                                                               >  6:5130/1‑149 (MQ=255)       aTCGGCACTGTCGAACAAGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAAAGCTGCGACCCTTCTGCGCAACAt                                                                                                                            >  6:235280/1‑149 (MQ=255)           gCACTGGCGAACAAGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGAt                                                                                                                        <  8:158539/149‑1 (MQ=255)                   gAACAAGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAAt                                                                                                                 <  6:215616/148‑1 (MQ=255)                      cAAGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCa                                                                                                             <  3:220010/149‑1 (MQ=255)                       aaGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAg                                                                                                            >  6:146384/1‑149 (MQ=255)                        aGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCTGCTGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGc                                                                                                           <  5:235280/149‑1 (MQ=255)                        aGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGc                                                                                                           <  3:161958/149‑1 (MQ=255)                          cgcCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGt                                                                                                         <  5:146384/149‑1 (MQ=255)                           gcCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGtc                                                                                                        >  4:174469/1‑149 (MQ=255)                             cAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGtctc                                                                                                      >  1:108355/1‑149 (MQ=255)                                 gTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGccc                                                                                                  <  8:10891/149‑1 (MQ=255)                                  tAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCt                                                                                                 <  1:252725/149‑1 (MQ=255)                                           cGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCg                                                                                        >  2:65317/1‑149 (MQ=255)                                            cgCTTGTTCGACAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCg                                                                                        <  4:80454/148‑1 (MQ=255)                                             gCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCgg                                                                                       >  5:196088/1‑148 (MQ=255)                                             gCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGc                                                                                      <  5:5130/149‑1 (MQ=255)                                               ttG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCaa                                                                                    >  7:39024/1‑149 (MQ=255)                                                   tCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGcc                                                                                 >  4:46905/1‑149 (MQ=255)                                                   tCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGc                                                                                  <  7:181923/148‑1 (MQ=255)                                                         ggcaAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCAAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTg                                                                           >  3:153054/1‑149 (MQ=255)                                                          gcaAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTgc                                                                          <  3:174469/149‑1 (MQ=255)                                                          gcaAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTgc                                                                          >  2:93796/1‑149 (MQ=255)                                                          gcaAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTgc                                                                          <  2:97638/149‑1 (MQ=255)                                                            aaGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCAAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTgcgc                                                                        <  4:153054/149‑1 (MQ=255)                                                               tCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGc                                                                                               >  1:290612/1‑123 (MQ=255)                                                                     aGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCACGCCGGCCTGCGCCCCCTTGaa                                                               >  2:301033/1‑149 (MQ=255)                                                                        aCCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGa                                                            >  1:180431/1‑149 (MQ=255)                                                                         cccGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGAc                                                           >  8:102726/1‑149 (MQ=255)                                                                                       tCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCaa                                                                                             <  8:246705/101‑1 (MQ=255)                                                                                       tCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCaa                                                                                             >  7:246705/1‑101 (MQ=255)                                                                                        cGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGa                                                 >  5:261110/1‑144 (MQ=255)                                                                                        cGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGa                                                 <  6:261110/144‑1 (MQ=255)                                                                                          gCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCcgcg                                          >  3:265853/1‑149 (MQ=255)                                                                                              cGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAg                                      >  3:166078/1‑149 (MQ=255)                                                                                                      aaGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGcc                              >  3:177339/1‑149 (MQ=255)                                                                                                        gcgcTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCa                            <  6:196088/149‑1 (MQ=255)                                                                                                          gcTCGGCGGTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGt                          <  4:177339/149‑1 (MQ=255)                                                                                                           cTCGGCGGTATCGATGTAACCGCGAATGCTGCGAACCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGtt                         >  4:161089/1‑149 (MQ=255)                                                                                                              ggcggTATCGAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCt                      <  7:102726/149‑1 (MQ=255)                                                                                                                       gAGGTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCTGGGTCGcc              <  5:153263/148‑1 (MQ=255)                                                                                                                          gTAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCTGGGTCGCCGTa           <  8:121186/148‑1 (MQ=255)                                                                                                                           tAACCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCTGGGTCGCCGTATc         <  3:161089/149‑1 (MQ=255)                                                                                                                            aaCCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCTGGGTCGCCGTATCg        >  6:144855/1‑149 (MQ=255)                                                                                                                             aCCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCTGGGTCGCCGTATCgg       <  3:46905/149‑1 (MQ=255)                                                                                                                             aCCGCGAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCTGGGTCGCCGTATCgg       >  2:3949/1‑149 (MQ=255)                                                                                                                               cgcgAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCTGGGTCGCCGTATCGGc      >  5:33415/1‑148 (MQ=255)                                                                                                                                  gAATGCTGCGACCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCTGGGTCGCCGTATCGGCCAAt  <  2:108355/149‑1 (MQ=255)                                                                                                                                            |                                                                                                                                           GCCGCCATCGGCACTGTCGAACAAGCGCCAGCGTAACCGTCCCGGCTTG‑TCGGCAGGCAAGTCGCCGAGCACCCGATATCGTGGCTCGGCACCGCTGCGCAAGCGCTCGGCGGTATCGAGGTAACCGCGAATGCTGCGGCCCTTCTGCGCAACATCGATGAACAATTCCAGCGTCTCGCCCTGCAAGCCCGGCAAGCCGGCCTGCGCCCCCTTGAACGACCAGCGCCAGATGCCGCGCAAGGTGTCGCCCAGTTGCTGGGTCGCCGTATCGGCCAAT  >  NC_002947/4759376‑4759652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑