Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,767,818 C→T 9.8% intergenic (+32/+37) fpvA → / ← PP_4218 TonB‑dependent outer membrane ferripyoverdine receptor FpvA/lipase/esterase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,767,8180CT 9.8% 138.6 / 5.0 51intergenic (+32/+37)fpvA/PP_4218TonB‑dependent outer membrane ferripyoverdine receptor FpvA/lipase/esterase family protein
Reads supporting (aligned to +/- strand):  ref base C (25/21);  new base T (2/3);  total (27/24)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

CGACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGAT  >  NC_002947/4767681‑4767956
                                                                                                                                         |                                                                                                                                          
cGACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtt                                                                                                                                  <  6:302102/148‑1 (MQ=255)
  aCAAGAAGTACTACCAGGGCATTGCCAGTAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACTCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACAGCACCCAGGTGCGGttttt                                                                                                                               >  2:248634/1‑149 (MQ=255)
  aCAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCgg                                                                                                                                    <  1:277172/144‑1 (MQ=255)
  aCAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCgg                                                                                                                                    >  2:277172/1‑144 (MQ=255)
  aCAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGt                                                                                                                                        >  1:225335/1‑140 (MQ=255)
  aCAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGt                                                                                                                                        <  2:225335/140‑1 (MQ=255)
   cAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCTCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGtttttt                                                                                                                              <  3:3279/149‑1 (MQ=255)
    aagaagTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTc                                                                                                                             <  3:192848/149‑1 (MQ=255)
      gaagTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCAt                                                                                                                           <  8:231924/149‑1 (MQ=255)
      gaagTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCAt                                                                                                                           <  4:139347/149‑1 (MQ=255)
              acCAGGGCATTGCCAGCATCAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAAGGCAAAAAACCGCACCTGGGTGCGGtttttt                                                                                                                              >  5:284146/1‑138 (MQ=255)
              acCAGGGCATTGCCAGCATCAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCTGGGTGCGGtttttt                                                                                                                              <  6:284146/138‑1 (MQ=255)
                cAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTg                                                                                                                  >  2:311060/1‑148 (MQ=255)
                           cAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCt                                                                                                       >  6:21062/1‑148 (MQ=255)
                            aGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTa                                                                                                     <  4:158142/149‑1 (MQ=255)
                            aGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTa                                                                                                     <  5:113403/149‑1 (MQ=255)
                                   acTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGAtgc                                                                                              <  5:21062/149‑1 (MQ=255)
                                     tCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGAtgctg                                                                                            >  8:27006/1‑149 (MQ=255)
                                            gCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGt                                                                                     <  1:311060/149‑1 (MQ=255)
                                              ccGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTccc                                                                                                        <  3:295922/128‑1 (MQ=255)
                                              ccGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTccc                                                                                                        >  4:295922/1‑128 (MQ=255)
                                              ccGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGc                                                                                   >  5:219981/1‑149 (MQ=255)
                                                                   ccACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGc                                                                    <  6:179627/143‑1 (MQ=255)
                                                                   ccACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGc                                                                    >  5:179627/1‑143 (MQ=255)
                                                                   ccACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGcc                                                              >  7:97965/1‑149 (MQ=255)
                                                                     aCGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGAtgctgctg                                                                                         >  2:205887/1‑120 (MQ=255)
                                                                     aCGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGAtgctgctg                                                                                         <  1:205887/120‑1 (MQ=255)
                                                                     aCGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCtt                                                            >  5:98740/1‑149 (MQ=255)
                                                                     aCGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCt                                                             >  2:9097/1‑148 (MQ=255)
                                                                        cAACTTCACGATCACCGCCAAGTACAGCTTCTGATCGCCTGAAGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCAt                                                         <  1:248634/149‑1 (MQ=255)
                                                                         aaCTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATc                                                        <  7:27006/149‑1 (MQ=255)
                                                                            ttCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGc                                                     >  5:47009/1‑149 (MQ=255)
                                                                                   tCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCctcctc                                               >  5:185878/1‑148 (MQ=255)
                                                                                       cGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGacca                                                                           >  7:121412/1‑116 (MQ=255)
                                                                                       cGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGacca                                                                           <  8:121412/116‑1 (MQ=255)
                                                                                       cGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCga                                             <  4:220302/146‑1 (MQ=255)
                                                                                       cGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCga                                             >  3:220302/1‑146 (MQ=255)
                                                                                         ccAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTccc                                                                                                        <  4:112436/85‑1 (MQ=255)
                                                                                         ccAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTccc                                                                                                        >  3:112436/1‑85 (MQ=255)
                                                                                         ccAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATc                                        >  2:47187/1‑149 (MQ=255)
                                                                                            aGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGaa                                     <  6:98740/149‑1 (MQ=255)
                                                                                                   cTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATAc                              >  3:137299/1‑149 (MQ=255)
                                                                                                   cTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATAGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATAc                              >  5:81573/1‑149 (MQ=255)
                                                                                                          tCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGaa                       <  8:97965/149‑1 (MQ=255)
                                                                                                                gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATg                 >  2:63546/1‑149 (MQ=255)
                                                                                                                         cGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTcc        <  1:9097/149‑1 (MQ=255)
                                                                                                                            aaaaaaCCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCgg     >  6:306837/1‑149 (MQ=255)
                                                                                                                            aaaaaaCCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCgg     >  8:65623/1‑149 (MQ=255)
                                                                                                                               aaaCCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCAGCGCCCGGCCTTCATCGGCCGCCGCGATGATCGCACCCATACGCATGAAGGCAGGGGGCCCTCCCggggt  >  8:206101/1‑147 (MQ=255)
                                                                                                                                    gCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCg                                                                               >  2:262465/1‑67 (MQ=255)
                                                                                                                                    gCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCg                                                                               <  1:262465/67‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CGACAAGAAGTACTACCAGGGCATTGCCAGCAACAACTCCTGGAGCCCGTACGACGTGTATGGTGACCCACGCAACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTCATGGATGCCTGAAAGGCTCCCTTACAGATGCTGCTGCAGTGCCTCGACCACCATCGCCAGGCCTTCATCGGCCTCCTCGATGATCGAACCCATACGCATGAAGTCATGGGTCACTCCCGGGAT  >  NC_002947/4767681‑4767956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑