Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,797,609 T→A 7.0% N125I (AAC→ATC)  PP_4224 ← sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,797,6090TA 7.0% 165.2 / 3.1 57N125I (AAC→ATC) PP_4224sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (24/29);  new base A (2/2);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

CAGGCCCTTGCCAAGGCCGATCCACAACAGCCCCAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAGGCCATGCCATCCGGTA  >  NC_002947/4797461‑4797756
                                                                                                                                                    |                                                                                                                                                   
cAGGCCCTTGCCAAGGCCGATCCACAACAGCCCCAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGt                                                                                                                                                     >  2:105416/1‑149 (MQ=255)
 aGGCCCTTGCCAAGGCCGATCCACAACAGCCCCAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGtt                                                                                                                                                    <  8:15282/149‑1 (MQ=255)
   gCCCTTGCCAAGGCCGATCCACAACAGCCCCAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGt                                                                                                                                                  >  6:52805/1‑149 (MQ=255)
     ccTTGCCAAGGCCGATCCACAACAGCCCCAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAg                                                                                                                                                <  5:292113/149‑1 (MQ=255)
     ccTTGCCAAGGCCGATCCACAACAGCCCCAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAg                                                                                                                                                <  5:52805/149‑1 (MQ=255)
           cAAGGCCGATCCACAACAGCCCCAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAg                                                                                                                                          >  1:25926/1‑149 (MQ=255)
                       acaacaGCCCCAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAgc                                                                                                                              <  8:92727/149‑1 (MQ=255)
                              ccccAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCAtcgt                                                                                                                       <  1:133072/149‑1 (MQ=255)
                               cccAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGATCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCAtcgtc                                                                                                                      >  7:244384/1‑149 (MQ=255)
                                ccAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGGCAGCCGTGGTGATGCGCACATCGCCGTGGATGTAGGTAAAGGTGCGCCAAAGCGCAtcgtc                                                                                                                      >  3:236857/1‑148 (MQ=255)
                                ccAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGACAGCCGTGGTGATGCGCACATCGCCGTGGATGTAGGTAAAGGTGCGCCAAAGCGCAtcgtc                                                                                                                      >  3:232661/1‑148 (MQ=255)
                                     cTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGAtggt                                                                                                                <  8:246810/149‑1 (MQ=255)
                                         cGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGAtggtctgg                                                                                                            >  5:81082/1‑149 (MQ=255)
                                          gAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGAtggtctggt                                                                                                           <  3:209286/149‑1 (MQ=255)
                                           aGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGAtggtctggtc                                                                                                          <  3:245749/149‑1 (MQ=255)
                                                       aGTCCCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCt                                                                                              <  6:229526/149‑1 (MQ=255)
                                                       aGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCt                                                                                              >  6:49573/1‑149 (MQ=255)
                                                         tACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAg                                                                                                                                                <  7:216461/97‑1 (MQ=255)
                                                         tACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAg                                                                                                                                                >  8:216461/1‑97 (MQ=255)
                                                            cGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCt                                                                                         >  8:236723/1‑149 (MQ=255)
                                                             ggCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTTATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCtc                                                                                        <  7:138212/149‑1 (MQ=255)
                                                             ggCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCtc                                                                                        >  5:6132/1‑149 (MQ=255)
                                                              gcgcCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAgcgc                                                                                                                            >  4:114187/1‑112 (MQ=255)
                                                              gcgcCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAgcgc                                                                                                                            <  3:114187/112‑1 (MQ=255)
                                                              gcgcCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCtcg                                                                                       >  8:100317/1‑149 (MQ=255)
                                                                  cGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTcgtcgt                                                                                   >  3:226669/1‑149 (MQ=255)
                                                                   gATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCgtcgtc                                                                                  >  4:204316/1‑149 (MQ=255)
                                                                   gATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCgtcgtc                                                                                  >  4:273115/1‑149 (MQ=255)
                                                                        cgccAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATgg                                                                             >  1:318547/1‑149 (MQ=255)
                                                                              cagAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCACCGTCGATGGTCTGGTCACGAAACCCGCTGCGCtcg                                                                                       >  3:68500/1‑133 (MQ=255)
                                                                              cagAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCACCGTCGATGGTCTGGTCACGAAACCCGCTGCGCtcg                                                                                       <  4:68500/133‑1 (MQ=255)
                                                                               agacccGACTCGGTCAGCGCCGCGCGCTCCATGTGCCGGTCCGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATc                                                                      <  8:205346/144‑1 (MQ=255)
                                                                                gAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCg                                                                     >  3:297460/1‑149 (MQ=255)
                                                                                  aTCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTg                                                                   <  6:218759/149‑1 (MQ=255)
                                                                                          gTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTgg                                                            <  7:236723/148‑1 (MQ=255)
                                                                                          gTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGATGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTtgtg                                                             <  4:236857/147‑1 (MQ=255)
                                                                                          gTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGATGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTtgtg                                                             <  4:232661/147‑1 (MQ=255)
                                                                                              aGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGc                                                        <  2:318547/148‑1 (MQ=255)
                                                                                                   cTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGa                                                  <  8:244384/149‑1 (MQ=255)
                                                                                                      gcgcTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAgg                                                <  3:90054/148‑1 (MQ=255)
                                                                                                      gcgcTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAg                                                 <  6:81082/147‑1 (MQ=255)
                                                                                                      gcgcTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGa                                               <  2:25926/149‑1 (MQ=255)
                                                                                                      gcgcTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCCCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAgg                                                <  1:105416/148‑1 (MQ=255)
                                                                                                       cgcTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGGGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGAt                                              <  8:99751/149‑1 (MQ=255)
                                                                                                          tCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGAtctc                                           >  2:791/1‑149 (MQ=255)
                                                                                                            cATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGc                                         <  3:204316/149‑1 (MQ=255)
                                                                                                             aTGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCtt                                                                >  7:65792/1‑125 (MQ=255)
                                                                                                             aTGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCtt                                                                <  8:65792/125‑1 (MQ=255)
                                                                                                                    ggTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGc                                 <  6:6132/149‑1 (MQ=255)
                                                                                                                                  aTGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCa                   <  3:273115/149‑1 (MQ=255)
                                                                                                                                    gcgcACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAgg                 >  5:38610/1‑149 (MQ=255)
                                                                                                                                    gcgcACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAgg                 >  1:31679/1‑149 (MQ=255)
                                                                                                                                    gcgcACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAgg                 >  1:291034/1‑149 (MQ=255)
                                                                                                                                        acaTCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAGgccat             >  6:207529/1‑149 (MQ=255)
                                                                                                                                         caTCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAGgccatg            >  4:315332/1‑149 (MQ=255)
                                                                                                                                                gTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAGGCCATGCCATCCg     <  6:38610/149‑1 (MQ=255)
                                                                                                                                                gTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAGGCCATGCCATCCg     <  2:291034/149‑1 (MQ=255)
                                                                                                                                                 tGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAGGCCATGCCATCCgg    <  4:297460/149‑1 (MQ=255)
                                                                                                                                                   gTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAGGCCATGCCATCCGGTa  >  2:11254/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGGCCCTTGCCAAGGCCGATCCACAACAGCCCCAGGCTGCCGAGCAGGGCCATCAGTACCGGCGCCGATGCCGCCAGCAGAATCGACTGGTTCAGCGCCTCGCGCTCCATGTGCCGGTCAGCCGTGGTGATGCGCACATCGCCGTGGTTGTAGGTAAAGGTGCGCCAAAGCGCATCGTCGATGGTCTGGTCACGAAACCCGCTGCGCTCGTCGTCCATGGCGCCATCGTGCTTGTGGTTGCTGGCGAGGATCTCGCCACGCAGCGAGCTGACCTGGCAGGCCATGCCATCCGGTA  >  NC_002947/4797461‑4797756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑