Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,822,513 G→A 7.4% R2893C (CGT→TGT)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,822,5130GA 7.4% 157.8 / 2.1 54R2893C (CGT→TGT) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (26/24);  new base A (2/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.25e-01

CGACGGCGTGGGTCTTGGCCGGCAGAGGCTGGCCACGGTACAGCGCCTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCACGTACTGGGCCTGCCA  >  NC_002947/4822375‑4822660
                                                                                                                                          |                                                                                                                                                   
cGACGGCGTGGGTCTTGGCCGGCAGAGGCTGGCCACGGTACAGCGCCTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAg                                                                                                                                           <  1:49169/149‑1 (MQ=255)
       gTGGGTCTTGGCCGGCAGAGGCTGGCCACGGTACAGCGCCTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCgca                                                                                                                                    <  8:223879/149‑1 (MQ=255)
                       agagGCTGGCCACGGTACAGCGCCTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTg                                                                                                                    >  7:270920/1‑149 (MQ=255)
                               gCCACGGTACAGCGCCTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTc                                                                                                              >  6:36358/1‑147 (MQ=255)
                                         aGCGCCTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGgctgc                                                                                                      <  6:195165/145‑1 (MQ=255)
                                         aGCGCCTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGgctgc                                                                                                      >  5:195165/1‑145 (MQ=255)
                                           cgcCTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCa                                                                                                <  1:207489/149‑1 (MQ=255)
                                           cgcCTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCa                                                                                                >  8:127301/1‑149 (MQ=255)
                                             ccTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACg                                                                                              <  7:239185/149‑1 (MQ=255)
                                                 cAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGc                                                                                                                   >  1:171767/1‑124 (MQ=255)
                                                 cAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGc                                                                                                                   <  2:171767/124‑1 (MQ=255)
                                                      cTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACTTCg                                                                                           <  3:20697/143‑1 (MQ=255)
                                                      cTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCg                                                                                           >  4:20697/1‑143 (MQ=255)
                                                       ttCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCATCAAGGCGCGCAGCAGAGGACCCTCTTGCAGTTCCACGCTGCGCTGAACGTAGGTGTa                                                                                      >  5:177609/1‑147 (MQ=255)
                                                       ttCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTa                                                                                      >  6:130131/1‑147 (MQ=255)
                                                          cagcagCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTg                                                                                 >  8:292996/1‑149 (MQ=255)
                                                           agcagcACCCGCCAGGACACGCCCTCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGGCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGc                                                                                <  7:99719/149‑1 (MQ=255)
                                                           agcagcACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGc                                                                                <  8:270920/149‑1 (MQ=255)
                                                               gcaCCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGgt                                                                                         >  5:161826/1‑136 (MQ=255)
                                                               gcaCCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGgt                                                                                         <  6:161826/136‑1 (MQ=255)
                                                                caCCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGa                                                                            <  5:182832/148‑1 (MQ=255)
                                                                caCCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGa                                                                            >  6:182832/1‑148 (MQ=255)
                                                                       catgACACGCCATCAACATCCAGTTGATGAATCGCCAGCTTCAACCGCTGGGTGGCCTGCGCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGTTCCAGGCTGCGCTGCACGTCTGTGTAGAGTGCCTGACAATCAtt                                                                    <  6:177609/146‑1 (MQ=255)
                                                                                 cATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGc                                                           >  8:276236/1‑148 (MQ=255)
                                                                                  aTCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACACACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGa                                                         >  8:68414/1‑149 (MQ=255)
                                                                                   tCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGa                                                         >  2:13634/1‑148 (MQ=255)
                                                                                     aacaacCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAg                                                      >  8:144167/1‑149 (MQ=255)
                                                                                       caacCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTg                                                    >  6:174403/1‑149 (MQ=255)
                                                                                        aacCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTgg                                                   <  7:292996/149‑1 (MQ=255)
                                                                                           cAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACACACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGgcgc                                                <  7:68414/149‑1 (MQ=255)
                                                                                            aGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCt                                               >  5:62799/1‑149 (MQ=255)
                                                                                                 atgaATCGCCAGCAGCAACCGATGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCTGCCCATGTTGCAGGTCCATGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCcaca                                          <  5:130131/149‑1 (MQ=255)
                                                                                                   gaATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTg                                                                                                                    >  8:282691/1‑73 (MQ=255)
                                                                                                   gaATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTg                                                                                                                    <  7:282691/73‑1 (MQ=255)
                                                                                                     aTCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCAcagcag                                      >  5:291103/1‑149 (MQ=255)
                                                                                                         ccAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCAcagcag                                      <  1:6735/145‑1 (MQ=255)
                                                                                                         ccAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCAcagcag                                      >  2:6735/1‑145 (MQ=255)
                                                                                                         ccAGCAGCAACCGCTGGGTGCCCTGCCCGTCACACACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGgcg                                                 >  6:52812/1‑134 (MQ=255)
                                                                                                         ccAGCAGCAACCGCTGGGTGCCCTGCCCGTCACACACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGgcg                                                 <  5:52812/134‑1 (MQ=255)
                                                                                                           agcagcAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTc                                              >  6:61836/1‑135 (MQ=255)
                                                                                                           agcagcAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTc                                              <  5:61836/135‑1 (MQ=255)
                                                                                                             cagcaACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCgg                              >  6:108597/1‑149 (MQ=255)
                                                                                                                 aaCCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGgcgc                           >  2:185660/1‑148 (MQ=255)
                                                                                                                   ccGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAg                        >  2:164142/1‑149 (MQ=255)
                                                                                                                    cGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAgg                       <  1:13634/149‑1 (MQ=255)
                                                                                                                         ggTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCa                  >  6:19402/1‑149 (MQ=255)
                                                                                                                          gTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCAc                 <  7:276236/149‑1 (MQ=255)
                                                                                                                              cctgcccGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCACGTAc             <  5:108597/149‑1 (MQ=255)
                                                                                                                               ctgcccGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCACGTACt            >  8:81110/1‑149 (MQ=255)
                                                                                                                                  cccGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCACGTACTgg          <  5:174403/148‑1 (MQ=255)
                                                                                                                                   ccGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCACGTACTGGGc        <  6:291103/149‑1 (MQ=255)
                                                                                                                                     gTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCACGTACTGGGCCt      >  7:193979/1‑149 (MQ=255)
                                                                                                                                     gTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCACGTACTGGGCCt      <  7:127301/149‑1 (MQ=255)
                                                                                                                                         cGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCACGTACTGGGCCTGCCa  <  7:144167/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                   
CGACGGCGTGGGTCTTGGCCGGCAGAGGCTGGCCACGGTACAGCGCCTGCAGGTCTTCCAGCAGCACCCGCCAGGACACGCCATCAACAACCAGGTGATGAATCGCCAGCAGCAACCGCTGGGTGCCCTGCCCGTCACGCACCAGCAAGGCGCGCAGCAGCGGCCCCTGTTGCAGGTCCAGGCTGCGCTGCACGTCGGTGTAGAGTGCCTGACAATCATTGAAATCGGCGACAGTGGCGCTCCACAGCAGTTGCTCGGCGCCAGGCTGCACGTACTGGGCCTGCCA  >  NC_002947/4822375‑4822660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑