Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,823,343 G→A 7.8% P2616L (CCG→CTG)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,823,3430GA 7.8% 145.6 / 2.2 51P2616L (CCG→CTG) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base A (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.69e-01

GTCGAGCAGCACCAGATGCGCAGGCACCATGTAGTCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACATACTCCACCAGGCCATCGGTA  >  NC_002947/4823195‑4823486
                                                                                                                                                    |                                                                                                                                               
gTCGAGCAGCACCAGATGCGCAGGCACCATGTAGTCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCg                                                                                                                                                 >  2:64136/1‑149 (MQ=255)
 tCGAGCAGCACCAGATGCGCAGGCACCATGTAGTCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCg                                                                                                                                                 <  6:214637/148‑1 (MQ=255)
     gcagcaCCAGATGCGCAGGCACCATGTAGTCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAg                                                                                                                                            >  2:320133/1‑149 (MQ=255)
        gcaCCAGATGCGCAGGCACCATGTAGTCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTc                                                                                                                                          >  5:67056/1‑148 (MQ=255)
           ccAGATGCGCAGGCACCATGTAGTCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAgc                                                                                                                                      <  4:77718/149‑1 (MQ=255)
                   gcAGGCACCATGTAGTCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCac                                                                                                                              >  5:304945/1‑149 (MQ=255)
                               tAGTCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCtcgtc                                                                                                                  <  1:64136/149‑1 (MQ=255)
                                 gTCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGa                                                                                                                <  2:254013/149‑1 (MQ=255)
                                  tCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTACCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGc                                                                                                                        <  3:35876/140‑1 (MQ=255)
                                  tCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTACCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGc                                                                                                                        >  4:35876/1‑140 (MQ=255)
                                     ggCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATc                                                                                                            <  2:222946/149‑1 (MQ=255)
                                       cAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTTCCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCt                                                                                                          >  1:93146/1‑149 (MQ=255)
                                           tGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTg                                                                                                      <  6:67056/149‑1 (MQ=255)
                                              tGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTgc                                                                                                     <  2:36270/147‑1 (MQ=255)
                                              tGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTgc                                                                                                     >  1:36270/1‑147 (MQ=255)
                                                 cGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAg                                                                                                <  3:267111/149‑1 (MQ=255)
                                                  gggCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAg                                                                                                >  1:147490/1‑148 (MQ=255)
                                                     cATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCCGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAgg                                                                                            >  5:29682/1‑149 (MQ=255)
                                                            tttCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGctct                                                                                     >  4:284948/1‑149 (MQ=255)
                                                               cACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATCGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCg                                                                                   <  4:240450/148‑1 (MQ=255)
                                                                 ccGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGAtt                                                                                >  4:87767/1‑149 (MQ=255)
                                                                   gCTTCGCGCAGCAGTTGTTGCAGGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTc                                                                              <  6:81447/149‑1 (MQ=255)
                                                                   gCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGGCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTc                                                                              <  7:147090/149‑1 (MQ=255)
                                                                   gCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTc                                                                              <  1:320133/149‑1 (MQ=255)
                                                                    cTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCg                                                                             <  6:304945/149‑1 (MQ=255)
                                                                                       cACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAg                                                                                                <  6:218526/111‑1 (MQ=255)
                                                                                       cACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAg                                                                                                >  5:218526/1‑111 (MQ=255)
                                                                                             gtTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCcgc                                                       <  8:17346/146‑1 (MQ=255)
                                                                                             gtTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCcgc                                                       >  7:17346/1‑146 (MQ=255)
                                                                                             gtTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCgg                                                     <  7:241837/148‑1 (MQ=255)
                                                                                             gtTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGa                                                    <  3:284948/149‑1 (MQ=255)
                                                                                                  cggcggTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTc                                               <  3:87767/149‑1 (MQ=255)
                                                                                                    gcggTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCAc                                             >  2:49147/1‑149 (MQ=255)
                                                                                                        tGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCAGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCACTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACctgc                                         >  6:61008/1‑149 (MQ=255)
                                                                                                            ctgcctgcACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGt                                     >  3:85999/1‑149 (MQ=255)
                                                                                                                ctgcACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTgcgc                                                                                                   <  3:139301/83‑1 (MQ=255)
                                                                                                                ctgcACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTgcgc                                                                                                   >  4:139301/1‑83 (MQ=255)
                                                                                                                  gcACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGc                                                                >  1:176751/1‑116 (MQ=255)
                                                                                                                  gcACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGc                                                                <  2:176751/116‑1 (MQ=255)
                                                                                                                      acCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGcc                           >  8:117746/1‑149 (MQ=255)
                                                                                                                          gATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACa                       <  2:147490/149‑1 (MQ=255)
                                                                                                                            tAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACATa                     <  4:85999/149‑1 (MQ=255)
                                                                                                                              gCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACATACt                   >  3:295285/1‑149 (MQ=255)
                                                                                                                               cccACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACATACTc                  <  7:117746/149‑1 (MQ=255)
                                                                                                                                ccaccaGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGAc                        >  7:220648/1‑142 (MQ=255)
                                                                                                                                ccaccaGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGAc                        <  8:220648/142‑1 (MQ=255)
                                                                                                                                ccaccaGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACATACTc                  >  3:61476/1‑148 (MQ=255)
                                                                                                                                     aGCTGCTTGCCGCCCAGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACgg                             <  7:302701/132‑1 (MQ=255)
                                                                                                                                     aGCTGCTTGCCGCCCAGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACgg                             >  8:302701/1‑132 (MQ=255)
                                                                                                                                      gctgctTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACATACTCCACCAgg           <  2:93146/149‑1 (MQ=255)
                                                                                                                                      gctgctTGCCGCCCAGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCACTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACATACTCCACCAgg           <  5:61008/149‑1 (MQ=255)
                                                                                                                                              ccgccCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACATACTCCACCAGGCCATCgg    >  6:174765/1‑148 (MQ=255)
                                                                                                                                               cgccCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACATACTCCACCAGGCCATCGGTa  >  5:238483/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GTCGAGCAGCACCAGATGCGCAGGCACCATGTAGTCGGGCAGGTGCTGGCGGGCATCGGCTTTCACCGCTTCGCGCAGCAGTTGTTGCACGTCGTTACCGGCGGTGGCCTGCCTGCACACCAGATAGCCCACCAGCTGCTTGCCGCCCGGCAAGTCCAGCGCCAGCACGACGGCCTCGTCGACATCCTCGTGCGCTAGCAGGCGGCTCTCGATTTCGCCCAGTTCGATGCGGAAGCCGCGGATCTTCACCTGCTGGTCGGCACGGCCGACATACTCCACCAGGCCATCGGTA  >  NC_002947/4823195‑4823486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑