Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,824,272 G→C 17.3% S2306R (AGC→AGG)  pvdL ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,824,2720GC17.3% 117.8 / 20.1 52S2306R (AGC→AGG) pvdLnon‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (20/23);  new base C (4/5);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.80e-01

ATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTACTCGGGGTC  >  NC_002947/4824126‑4824418
                                                                                                                                                  |                                                                                                                                                   
aTCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCt                                                                                                                                                   >  8:52115/1‑149 (MQ=255)
   aCCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTggc                                                                                                                                                >  7:129893/1‑149 (MQ=255)
      gCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTggcggc                                                                                                                                             <  5:247388/149‑1 (MQ=255)
       ccTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGCCTggcggcc                                                                                                                                            <  3:210759/149‑2 (MQ=255)
           gCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCg                                                                                                                                         >  7:148843/1‑148 (MQ=255)
           gCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCg                                                                                                                                         <  2:149318/148‑1 (MQ=255)
            cAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGCCTggcggcctacag                                                                                                                                       <  2:206884/149‑7 (MQ=255)
             aGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCt                                                                                                                                      >  3:27991/1‑149 (MQ=255)
               tGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTc                                                                                                                                    <  7:52115/149‑1 (MQ=255)
                 cATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGTTTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCa                                                                                                                                  <  4:32063/149‑1 (MQ=255)
                 cATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCa                                                                                                                                  <  4:167782/149‑1 (MQ=255)
                             gCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTgg                                                                                                                      <  2:269087/149‑1 (MQ=255)
                                       accaccACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTc                                                                                                            <  3:185639/149‑1 (MQ=255)
                                                           gCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGgtg                                                                                         <  3:95227/148‑1 (MQ=255)
                                                            ccGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGtgtg                                                                                       <  2:188929/149‑1 (MQ=255)
                                                               gTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGACAGGCTGGCGGCATCGTCTTACAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTg‑cag                                                                                   >  3:273529/1‑149 (MQ=255)
                                                                    gCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAgcg                                                                              >  1:272113/1‑149 (MQ=255)
                                                                       tGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCAc                                                                           >  7:230240/1‑149 (MQ=255)
                                                                            tgtAGATCAGGGAAGCCTGGGGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCACCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGCGTGCG‑CTGCTGCACACcctgg                                                                      <  8:281619/149‑1 (MQ=255)
                                                                                  tCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGagc                                                                <  2:194578/149‑1 (MQ=255)
                                                                                    aGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGagca                                                               <  8:148843/148‑1 (MQ=255)
                                                                                            cTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGagcag                                                              >  8:170222/1‑141 (MQ=255)
                                                                                            cTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGagcag                                                              <  7:170222/141‑1 (MQ=255)
                                                                                                tgctgcGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGtgtg                                                                                       <  2:59385/113‑1 (MQ=255)
                                                                                                tgctgcGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGtgtg                                                                                       >  1:59385/1‑113 (MQ=255)
                                                                                                        cAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCtt                                          >  2:90779/1‑149 (MQ=255)
                                                                                                        cAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCtt                                          <  8:129893/149‑1 (MQ=255)
                                                                                                             tGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGgtgt                                                                                        <  2:290075/99‑1 (MQ=255)
                                                                                                             tGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGgtgt                                                                                        >  1:290075/1‑99 (MQ=255)
                                                                                                             tGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGAt                                      >  1:193253/1‑148 (MQ=255)
                                                                                                              gAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCa                                    >  3:316878/1‑149 (MQ=255)
                                                                                                               aGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCAt                                   >  7:237808/1‑149 (MQ=255)
                                                                                                                 gTTGTCCAGCAGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATg                                  <  5:270847/148‑1 (MQ=255)
                                                                                                                 gTTGTCCAGCAGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATg                                  >  6:270847/1‑148 (MQ=255)
                                                                                                                  ttGTCCTGCTGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGGTCCAGCTGGCCACGCCTTCAGGGAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTa                                <  5:85776/149‑1 (MQ=255)
                                                                                                                     tCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTGCCAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAgc                              <  1:90779/149‑1 (MQ=255)
                                                                                                                               cATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTc                    >  6:61351/1‑148 (MQ=255)
                                                                                                                               cATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTc                    <  8:237808/148‑1 (MQ=255)
                                                                                                                                     tGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCggg             >  7:24465/1‑149 (MQ=255)
                                                                                                                                      gTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGc                                                   >  6:147437/1‑110 (MQ=255)
                                                                                                                                      gTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGc                                                   <  5:147437/110‑1 (MQ=255)
                                                                                                                                       tAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTa           >  2:308225/1‑149 (MQ=255)
                                                                                                                                       tAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTa           >  8:27958/1‑149 (MQ=255)
                                                                                                                                       gatgccgccAGCCTGGCGGCATCGTCTTACAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTa           >  5:114750/4‑149 (MQ=255)
                                                                                                                                        atgccgccAGCCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCgg                                                 <  7:274472/108‑1 (MQ=255)
                                                                                                                                        atgccgccAGCCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCgg                                                 >  8:274472/3‑110 (MQ=255)
                                                                                                                                        atgccgccAGCCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCAt                                   >  3:69325/3‑124 (MQ=255)
                                                                                                                                        atgccgccAGCCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCAt                                   <  4:69325/122‑1 (MQ=255)
                                                                                                                                        atgccgccAACCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCgg                                                 <  1:279194/108‑1 (MQ=255)
                                                                                                                                        atgccgccAACCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCgg                                                 >  2:279194/3‑110 (MQ=255)
                                                                                                                                            cgccAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTACTCgg      <  5:61351/149‑1 (MQ=255)
                                                                                                                                                aGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTACTCGGGGTc  <  2:193253/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
ATCACCGCCTGGCAGTGCATGGCGAACTCGCCATGGCTGACCACCACGCCCTTGGGTTTGCCGGTGGAGCCTGAGGTGTAGATCAGGTAAGCCTGGTGCTGCGGCAGGCTGAGGTTGTCCAGCGGTGCATCGCTGTAGGCCGCCAGGCTGGCGGCATCGTCTTCCAGGCTCCAGCTGGCCACGCCTTCAGGCAGCTCGCCCAGGGTGTG‑CAGCAGCGCACGCTGGCTGAGCAGCAAGCCGATGCGGCTGTCTTCGATCATGTAGCGCAGGCGGTCGAGCGGGTACTCGGGGTC  >  NC_002947/4824126‑4824418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑