Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,840,143 T→C 5.6% V170A (GTG→GCG)  ccoN‑II → cbb3‑type cytochrome c oxidase subunit 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,840,1430TC 5.6% 207.4 / 2.3 71V170A (GTG→GCG) ccoN‑IIcbb3‑type cytochrome c oxidase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (36/31);  new base C (2/2);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01

CCAAGGAATACGCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGTACGGCCACAACGCCGTGGGCTTCTTC  >  NC_002947/4839996‑4840276
                                                                                                                                                   |                                                                                                                                     
ccaAGGAATACGCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTg                                                                                                                                      <  3:227514/149‑1 (MQ=255)
 caAGGAATACGCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTgg                                                                                                                                     >  5:242864/1‑149 (MQ=255)
 caAGGAATACGCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTgg                                                                                                                                     >  5:91349/1‑149 (MQ=255)
 caAGGAATACGCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGCgg                                                                                                                                     >  6:286767/1‑149 (MQ=255)
        tACGCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACcgc                                                                                                                              >  1:277425/1‑149 (MQ=255)
           gCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTc                                                                                                                                   <  4:201617/141‑1 (MQ=255)
           gCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTc                                                                                                                                   >  3:201617/1‑141 (MQ=255)
           gCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCCCCGCGAt                                                                                                                           >  8:227452/1‑149 (MQ=255)
           gCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGAt                                                                                                                           <  6:242864/149‑1 (MQ=255)
           gCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCATCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGAt                                                                                                                           >  4:92652/1‑149 (MQ=255)
             cGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTGATCGTGGTCACCGCGAtgc                                                                                                                         <  2:94422/149‑1 (MQ=255)
              gAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGCGGTGACCACGAtgaa                                                                                                                        <  5:286767/149‑3 (MQ=255)
               aGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGAtgctg                                                                                                                       >  4:159348/1‑149 (MQ=255)
               aGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGAtgctg                                                                                                                       <  6:91349/149‑1 (MQ=255)
                   ggAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGCGGTGACCACGAtgaacgca                                                                                                                    >  5:313493/1‑142 (MQ=255)
                   ggAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGCGGTGACCACGAtgaacgca                                                                                                                    <  6:313493/148‑7 (MQ=255)
                      gTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCg                                                                                                                >  6:210707/1‑149 (MQ=255)
                        ggCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTc                                                                                                              <  4:60492/149‑1 (MQ=255)
                          ccGCTGGCGATCCTGCTGGCCATCGTCTTGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCaa                                                                                                            <  5:98092/149‑1 (MQ=255)
                            gCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAAc                                                                                                           >  1:190976/1‑148 (MQ=255)
                            gCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAAc                                                                                                           >  5:245114/1‑148 (MQ=255)
                             cTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAAcc                                                                                                          >  3:23282/1‑148 (MQ=255)
                                gCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCACCTGGTTCAACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACcaaa                                                                                                       >  6:173040/1‑146 (MQ=255)
                                gCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACcaca                                                                                                       >  3:266398/1‑148 (MQ=255)
                                   aTCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGTCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCTATGCTGCACATCGTCAACCACAtctc                                                                                                   <  1:153937/149‑1 (MQ=255)
                                   aTCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACAtctc                                                                                                   <  3:93531/149‑1 (MQ=255)
                                         ctgGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGcc                                                                                             <  7:154950/149‑1 (MQ=255)
                                         ctgGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGcc                                                                                             >  8:154950/1‑149 (MQ=255)
                                         ctgGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGc                                                                                              <  4:266398/148‑1 (MQ=255)
                                           ggCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCgg                                                                                           >  1:206705/1‑149 (MQ=255)
                                              cATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGa                                                                                        <  6:245114/149‑1 (MQ=255)
                                                     tGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACGAGATCTCGATGCAGGTGAGCCTGtt                                                                                 <  2:190976/149‑1 (MQ=255)
                                                       ggTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTc                                                                                >  3:51439/1‑148 (MQ=255)
                                                        gTTACCTACTGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCaa                                                                              <  1:91677/149‑1 (MQ=255)
                                                           aCCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGt                                                                            <  3:92652/148‑1 (MQ=255)
                                                              tACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTa                                                                        >  5:17567/1‑149 (MQ=255)
                                                               aCGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACTACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTAc                                                                       >  3:212430/1‑149 (MQ=255)
                                                                    cgggTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTGCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGc                                                                  <  2:183908/147‑1 (MQ=255)
                                                                      ggTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGt                                                                                          >  6:51682/1‑123 (MQ=255)
                                                                      ggTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGt                                                                                          <  5:51682/123‑1 (MQ=255)
                                                                       gtgtTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCt                                                                >  2:85067/1‑148 (MQ=255)
                                                                        tgtTCTTCGGCACTATCGTCAAACGCACGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTAc                                                              <  5:199829/149‑1 (MQ=255)
                                                                             ttcGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCgg                                                         >  6:36081/1‑149 (MQ=255)
                                                                                  cACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCa                                                    <  4:23282/149‑1 (MQ=255)
                                                                                         gTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGAcg                                              <  5:210707/148‑1 (MQ=255)
                                                                                          tCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGAcgc                                             >  1:133694/1‑148 (MQ=255)
                                                                                           cAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCga                                           <  2:277425/149‑1 (MQ=255)
                                                                                                gCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATc                                      <  2:206705/149‑1 (MQ=255)
                                                                                                gCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATc                                      >  1:188549/1‑149 (MQ=255)
                                                                                                  aaGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATc                                      >  2:109488/1‑147 (MQ=255)
                                                                                                  aaGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATc                                      >  8:165531/1‑147 (MQ=255)
                                                                                                    gACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGt                                                                                          >  7:32676/1‑93 (MQ=255)
                                                                                                    gACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGt                                                                                          <  8:32676/93‑1 (MQ=255)
                                                                                                     aCCAAGCTCTTCTATGTCGGCAACTGGTTCTACGGTTCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACTTCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAgtg                                 <  5:173040/149‑1 (MQ=255)
                                                                                                     aCCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAg                                                                             >  6:293853/1‑105 (MQ=255)
                                                                                                     aCCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAg                                                                             <  5:293853/105‑1 (MQ=255)
                                                                                                      ccAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAgtgg                                <  1:85067/149‑1 (MQ=255)
                                                                                                         aGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCAACCGACGCGATGATCCAGtggtgg                             >  8:76165/1‑149 (MQ=255)
                                                                                                          gCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTggtggt                            <  7:165531/149‑1 (MQ=255)
                                                                                                              aTCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGTACgg                        >  2:281660/1‑149 (MQ=255)
                                                                                                              aTCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGTACgg                        >  4:92877/1‑149 (MQ=255)
                                                                                                                  aTGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGTACGGCca                     >  7:98826/1‑148 (MQ=255)
                                                                                                                  aTGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTCCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGTACGGCca                     >  6:70760/1‑148 (MQ=255)
                                                                                                                   tGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAgtgg                                >  6:208332/1‑136 (MQ=255)
                                                                                                                   tGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAgtgg                                <  5:208332/136‑1 (MQ=255)
                                                                                                                   tGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGTACGGCcac                    <  3:159348/148‑1 (MQ=255)
                                                                                                                     tCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGTACGGCCACAAc                 >  4:156917/1‑149 (MQ=255)
                                                                                                                             tGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGTACGGCCACAACGCCGTgg          >  5:51479/1‑148 (MQ=255)
                                                                                                                                  cTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGa                                                >  7:94863/1‑105 (MQ=255)
                                                                                                                                  cTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGa                                                <  8:94863/105‑1 (MQ=255)
                                                                                                                                     cGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGTACGGCCACAACGCCGTGGGCttcttc  <  1:109488/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                     
CCAAGGAATACGCCGAGCTGGAGTGGCCGCTGGCGATCCTGCTGGCCATCGTCTGGGTTACCTACGGGCTGGTGTTCTTCGGCACTATCGTCAAACGCAAGACCAAGCACATCTATGTCGGCAACTGGTTCTACGGTGCGTTCATCGTGGTCACCGCGATGCTGCACATCGTCAACCACATCTCGCTGCCGGTGAGCCTGTTCAAGTCGTACTCGGCCTACTCCGGCGCCACCGACGCGATGATCCAGTGGTGGTACGGCCACAACGCCGTGGGCTTCTTC  >  NC_002947/4839996‑4840276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑