Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,877,036 G→T 7.4% intergenic (‑84/‑316) pucM ← / → puuE 5‑hydroxyisourate hydrolase/allantoinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,877,0360GT 7.4% 157.1 / 4.1 54intergenic (‑84/‑316)pucM/puuE5‑hydroxyisourate hydrolase/allantoinase
Reads supporting (aligned to +/- strand):  ref base G (24/26);  new base T (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

CCTTGATCGAGCTACCCGGGCAGCCATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCAAACAGGCAG  >  NC_002947/4876889‑4877180
                                                                                                                                                    |                                                                                                                                                
ccTTGATCGAGCTACCCGGGCAGCCATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGt                                                                                                                                                 <  2:16545/149‑1 (MQ=255)
     aTCGAGCTACCCGGGCAGCCATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGttatt                                                                                                                                             <  6:243812/148‑1 (MQ=255)
        gAGCTACCCGGGCAGCCATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAg                                                                                                                                          >  8:161628/1‑148 (MQ=255)
              cccGGGCAGCCATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATtc                                                                                                                                    <  1:275444/148‑1 (MQ=255)
                        cATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGAt                                                                                                                         <  8:278407/149‑1 (MQ=255)
                         aTGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGTCTTAATAACGTCATtcccg                                                                                                                                 >  4:238064/1‑138 (MQ=255)
                         aTGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGTCTTAATAACGTCATtcccg                                                                                                                                 <  3:238064/140‑3 (MQ=255)
                              cgGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTaca                                                                                                                   <  4:222990/149‑1 (MQ=255)
                              cgGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTac                                                                                                                    >  2:158708/1‑148 (MQ=255)
                                  aTCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCAGATACCCAAAGCCAGTGGCGGCAGGCGGGAATGAAGTTATTATTACATTAAAACCAAGGATTGTagaaaa                                                                                                                >  1:271350/1‑144 (MQ=255)
                                       gTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAAtttttt                                                                                                          <  4:119402/149‑1 (MQ=255)
                                           gtgtgtGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTGATTAAGACATTCCATCAAAGGATTGTACACAATTTTTTGATc                                                                                                      >  7:135011/1‑149 (MQ=255)
                                                 ggTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGTCTTAATAACGTCATtcccgc                                                                                                                                >  4:282238/1‑114 (MQ=255)
                                                 ggTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGTCTTAATAACGTCATtcccgc                                                                                                                                <  3:282238/117‑4 (MQ=255)
                                                  gTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCt                                                                                                <  4:187626/148‑1 (MQ=255)
                                                  gTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTg                                                                                               >  2:222930/1‑149 (MQ=255)
                                                  gTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTg                                                                                               >  4:266429/1‑149 (MQ=255)
                                                   tCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGt                                                                                              >  1:14462/1‑149 (MQ=255)
                                                   tCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGt                                                                                              <  3:312676/149‑1 (MQ=255)
                                                     aaaCGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTca                                                                                            <  1:158708/149‑1 (MQ=255)
                                                       aCGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATtcc                                                                                                                                   >  4:51175/1‑108 (MQ=255)
                                                       aCGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATtcc                                                                                                                                   <  3:51175/108‑1 (MQ=255)
                                                       aCGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTcaca                                                                                          >  2:259737/1‑149 (MQ=255)
                                                       aCGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTcaca                                                                                          >  4:213006/1‑149 (MQ=255)
                                                           cccATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGa                                                                                                                                         >  8:45018/1‑98 (MQ=255)
                                                           cccATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGa                                                                                                                                         <  7:45018/98‑1 (MQ=255)
                                                             cATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGAtt                                                                                                                        <  8:184546/113‑1 (MQ=255)
                                                             cATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGAtt                                                                                                                        >  7:184546/1‑113 (MQ=255)
                                                                        gCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGcc                                                                          >  1:308243/1‑148 (MQ=255)
                                                                         ccTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCtt                                                                                                  <  4:252736/123‑1 (MQ=255)
                                                                         ccTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCtt                                                                                                  >  3:252736/1‑123 (MQ=255)
                                                                            ctTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATa                                                                      >  5:290162/1‑148 (MQ=255)
                                                                               gTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATaaa                                                                    <  3:266429/147‑1 (MQ=255)
                                                                               gTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCCGCAGGCGGGAATGACGTTATTAAGAAATTCCATCCAAGGATTGTACACAATATTTTGATCCCTACAGTCACACCCTCCATTTGCTTCCCATaaaa                                                                   >  7:194698/1‑147 (MQ=255)
                                                                                 tCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGAc                                                                >  3:20678/1‑149 (MQ=255)
                                                                                     tCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCAt                                                            >  6:55244/1‑149 (MQ=255)
                                                                                         gcggCAAAAAATACTCACCCATCGCACCAGCTTAGCCCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGt                                                        <  8:95288/147‑1 (MQ=255)
                                                                                          ggtcAATAATTACTCATCGATCGCAGCAGGTTAGCGCCT‑GCGGCAGGCTGGAATGAGGTTTTTATGACATTCCATCCTAGGATTGTACTCAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGt                                                        <  8:194698/145‑1 (MQ=255)
                                                                                             cAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGc                                                     >  6:322924/1‑148 (MQ=255)
                                                                                               aaaaaTACTCAGCCATCGCAACATCTTATCGCCT‑GCGGCATGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCaaa                                                  <  7:161628/149‑1 (MQ=255)
                                                                                                   aTACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATaca                                              <  3:213006/149‑1 (MQ=255)
                                                                                                      cTCAGCCATCGCACGAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGc                                           <  5:320660/149‑1 (MQ=255)
                                                                                                       tCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCa                                          >  2:309160/1‑149 (MQ=255)
                                                                                                         cgCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCCCACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCAc                                         >  6:165918/2‑148 (MQ=255)
                                                                                                           ccATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCAt                                      <  5:322924/149‑1 (MQ=255)
                                                                                                             aTCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATaa                                    >  2:321536/1‑149 (MQ=255)
                                                                                                                 cACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGAt                                <  1:222930/149‑1 (MQ=255)
                                                                                                                  aCCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATg                               >  7:185692/1‑149 (MQ=255)
                                                                                                                      gCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCaa                           <  2:308243/149‑1 (MQ=255)
                                                                                                                       cTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAAc                          <  3:214595/149‑1 (MQ=255)
                                                                                                                                    ggCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGa              <  6:290162/149‑1 (MQ=255)
                                                                                                                                         gCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCaaa         >  2:148962/1‑149 (MQ=255)
                                                                                                                                           gggAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCAAAca       <  1:148962/149‑1 (MQ=255)
                                                                                                                                                tGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCAAAcaggcag  <  1:321536/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CCTTGATCGAGCTACCCGGGCAGCCATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCAAACAGGCAG  >  NC_002947/4876889‑4877180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑