Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,877,043 A→C 7.6% intergenic (‑91/‑309) pucM ← / → puuE 5‑hydroxyisourate hydrolase/allantoinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,877,0430AC 7.6% 151.1 / 4.6 53intergenic (‑91/‑309)pucM/puuE5‑hydroxyisourate hydrolase/allantoinase
Reads supporting (aligned to +/- strand):  ref base A (23/24);  new base C (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GAGCTACCCGGGCAGCCATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCAAACAGGCAGGTTTCTTG  >  NC_002947/4876897‑4877188
                                                                                                                                                   |                                                                                                                                                 
gAGCTACCCGGGCAGCCATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAg                                                                                                                                                  >  8:161628/1‑148 (MQ=255)
      cccGGGCAGCCATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATtc                                                                                                                                            <  1:275444/148‑1 (MQ=255)
                cATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGAt                                                                                                                                 <  8:278407/149‑1 (MQ=255)
                 aTGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGTCTTAATAACGTCATtcccg                                                                                                                                         <  3:238064/140‑3 (MQ=255)
                 aTGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGTCTTAATAACGTCATtcccg                                                                                                                                         >  4:238064/1‑138 (MQ=255)
                      cgGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTaca                                                                                                                           <  4:222990/149‑1 (MQ=255)
                      cgGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTac                                                                                                                            >  2:158708/1‑148 (MQ=255)
                          aTCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCAGATACCCAAAGCCAGTGGCGGCAGGCGGGAATGAAGTTATTATTACATTAAAACCAAGGATTGTagaaaa                                                                                                                        >  1:271350/1‑144 (MQ=255)
                               gTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAAtttttt                                                                                                                  <  4:119402/149‑1 (MQ=255)
                                   gtgtgtGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTGATTAAGACATTCCATCAAAGGATTGTACACAATTTTTTGATc                                                                                                              >  7:135011/1‑149 (MQ=255)
                                         ggTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGTCTTAATAACGTCATtcccgc                                                                                                                                        >  4:282238/1‑114 (MQ=255)
                                         ggTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGTCTTAATAACGTCATtcccgc                                                                                                                                        <  3:282238/117‑4 (MQ=255)
                                          gTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCt                                                                                                        <  4:187626/148‑1 (MQ=255)
                                          gTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTg                                                                                                       >  2:222930/1‑149 (MQ=255)
                                          gTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTg                                                                                                       >  4:266429/1‑149 (MQ=255)
                                           tCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGt                                                                                                      <  3:312676/149‑1 (MQ=255)
                                           tCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGt                                                                                                      >  1:14462/1‑149 (MQ=255)
                                             aaaCGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTca                                                                                                    <  1:158708/149‑1 (MQ=255)
                                               aCGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATtcc                                                                                                                                           >  4:51175/1‑108 (MQ=255)
                                               aCGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATtcc                                                                                                                                           <  3:51175/108‑1 (MQ=255)
                                               aCGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTcaca                                                                                                  >  2:259737/1‑149 (MQ=255)
                                               aCGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTcaca                                                                                                  >  4:213006/1‑149 (MQ=255)
                                                   cccATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGa                                                                                                                                                 <  7:45018/98‑1 (MQ=255)
                                                   cccATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGa                                                                                                                                                 >  8:45018/1‑98 (MQ=255)
                                                     cATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGAtt                                                                                                                                >  7:184546/1‑113 (MQ=255)
                                                     cATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGAtt                                                                                                                                <  8:184546/113‑1 (MQ=255)
                                                                gCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGcc                                                                                  >  1:308243/1‑148 (MQ=255)
                                                                 ccTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCtt                                                                                                          >  3:252736/1‑123 (MQ=255)
                                                                 ccTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCtt                                                                                                          <  4:252736/123‑1 (MQ=255)
                                                                    ctTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATa                                                                              >  5:290162/1‑148 (MQ=255)
                                                                       gTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATaaa                                                                            <  3:266429/147‑1 (MQ=255)
                                                                       gTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCCGCAGGCGGGAATGACGTTATTAAGAAATTCCATCCAAGGATTGTACACAATATTTTGATCCCTACAGTCACACCCTCCATTTGCTTCCCATaaaa                                                                           >  7:194698/1‑147 (MQ=255)
                                                                         tCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGAc                                                                        >  3:20678/1‑149 (MQ=255)
                                                                             tCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCAt                                                                    >  6:55244/1‑149 (MQ=255)
                                                                                 gcggCAAAAAATACTCACCCATCGCACCAGCTTAGCCCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGt                                                                <  8:95288/147‑1 (MQ=255)
                                                                                  ggtcAATAATTACTCATCGATCGCAGCAGGTTAGCGCCT‑GCGGCAGGCTGGAATGAGGTTTTTATGACATTCCATCCTAGGATTGTACTCAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGt                                                                <  8:194698/145‑1 (MQ=255)
                                                                                     cAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGc                                                             >  6:322924/1‑148 (MQ=255)
                                                                                       aaaaaTACTCAGCCATCGCAACATCTTATCGCCT‑GCGGCATGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCaaa                                                          <  7:161628/149‑1 (MQ=255)
                                                                                           aTACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATaca                                                      <  3:213006/149‑1 (MQ=255)
                                                                                              cTCAGCCATCGCACGAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGc                                                   <  5:320660/149‑1 (MQ=255)
                                                                                               tCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCa                                                  >  2:309160/1‑149 (MQ=255)
                                                                                                 cgCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCCCACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCAc                                                 >  6:165918/2‑148 (MQ=255)
                                                                                                   ccATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCAt                                              <  5:322924/149‑1 (MQ=255)
                                                                                                     aTCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATaa                                            >  2:321536/1‑149 (MQ=255)
                                                                                                         cACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGAt                                        <  1:222930/149‑1 (MQ=255)
                                                                                                          aCCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATg                                       >  7:185692/1‑149 (MQ=255)
                                                                                                              gCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCaa                                   <  2:308243/149‑1 (MQ=255)
                                                                                                               cTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAAc                                  <  3:214595/149‑1 (MQ=255)
                                                                                                                            ggCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGa                      <  6:290162/149‑1 (MQ=255)
                                                                                                                                 gCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCaaa                 >  2:148962/1‑149 (MQ=255)
                                                                                                                                   gggAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCAAAca               <  1:148962/149‑1 (MQ=255)
                                                                                                                                        tGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCAAAcaggcag          <  1:321536/149‑1 (MQ=255)
                                                                                                                                                ttaAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTCTCACACCCTCCATTTGCTGCCCATAAACGAACCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCAAACAGGCAGGTTTCTTg  <  8:185692/149‑1 (MQ=255)
                                                                                                                                                   aGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTa                           <  2:228674/121‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GAGCTACCCGGGCAGCCATGCGCGGCATCCAGTACGTGTGTGGTCAAACGTCCCATTGCGTGTCGCCTCTTGTTCAATCTGTGGGCAAAAAATACTCAGCCATCGCACCAGCTTAGCGCCT‑GCGGCAGGCGGGAATGACGTTATTAAGACATTCCATCCAAGGATTGTACACAATTTTTTGATCCCTTCTGTCACACCCTCCATTTGCTGCCCATAAACGACCCATCGGTCGCAAATACACGCACCATAACGATGCCAACCTCATTAGCTGACCAAACAGGCAGGTTTCTTG  >  NC_002947/4876897‑4877188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑