| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,895,897 | G→A | 13.8% | V118I (GTC→ATC) ‡ | PP_4304 → | VIC family cation transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,895,897 | 0 | G | A | 13.8% | 139.7 / 12.4 | 58 | V118I (GTC→ATC) ‡ | PP_4304 | VIC family cation transporter |
| Reads supporting (aligned to +/- strand): ref base G (25/25); new base A (4/4); total (29/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.52e-01 | |||||||||||
CGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTCGGC > NC_002947/4895755‑4896043 | cGAATTCATCACCCGCTTGTTCTGTTAGCCCAAGGCGGTGCGCTATGCCTTCAGTTTCTACGGCCTGGGCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCacc < 3:1246/148‑3 (MQ=255)cGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACATCGACGCCCAGTACATGCTCATCATCCGGGTCATc > 4:1246/1‑148 (MQ=255)cGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATcc < 4:89924/149‑1 (MQ=255) cATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGAtga < 1:292904/149‑2 (MQ=255) cATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGAtga < 6:44151/149‑2 (MQ=255) cACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGCTCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtgctgc > 1:252182/1‑149 (MQ=255) aCCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtgctgc < 4:143945/148‑1 (MQ=255) cTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCGTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGAGCTGTACTACAGCGACGCCCAGTACCTGCTAATCATCAGGGTCATCAGGATGCTGCGGATCt > 8:114698/1‑148 (MQ=255) tACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGa < 6:85416/142‑1 (MQ=255) tACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGa > 5:85416/1‑142 (MQ=255) tACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTcc < 5:193420/148‑1 (MQ=255) tACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTcc < 6:293268/148‑1 (MQ=255) cTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgtg > 7:128606/1‑149 (MQ=255) cTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt < 8:53037/148‑1 (MQ=255) cTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt < 4:219763/148‑1 (MQ=255) tGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGatgatga > 3:130208/1‑135 (MQ=255) tGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGatgatga < 4:130208/136‑2 (MQ=255) tGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCAACCGGGTCATCCGGATGCTGCGGATCTTCCgtg > 4:173842/1‑148 (MQ=255) ccAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAg > 4:10840/1‑149 (MQ=255) aaGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCt > 4:4960/1‑149 (MQ=255) aaGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCt < 2:273999/149‑1 (MQ=255) gCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtg < 4:317968/121‑1 (MQ=255) gCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtg > 3:317968/1‑121 (MQ=255) cgcTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt > 2:254348/1‑129 (MQ=255) cgcTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt < 1:254348/129‑1 (MQ=255) gCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGAtga > 3:293665/1‑108 (MQ=255) gCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGAtga < 4:293665/109‑2 (MQ=255) cTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGAt < 3:173842/149‑1 (MQ=255) gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGc > 3:309690/1‑134 (MQ=255) gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGc < 4:309690/134‑1 (MQ=255) gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATg > 8:213613/1‑147 (MQ=255) gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATg > 2:317141/1‑147 (MQ=255) gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATg > 6:42464/1‑147 (MQ=255) aTTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGgcg > 1:19238/1‑149 (MQ=255) gCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTgcgc < 7:213613/147‑1 (MQ=255) gCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGAGCTGCGCgg > 3:317279/1‑149 (MQ=255) gATCCTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCgg > 6:333000/1‑147 (MQ=255) ccGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTGATCATCCGGGTCATCCGGTTGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGTCCCATTACCTGATGGAGGCGCTGCGCGGCagcaagc < 2:252182/148‑1 (MQ=255) ggATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGTGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGACGCGCTGCGCGGCAGCAAGCag > 3:268322/1‑146 (MQ=255) ggATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCagaag < 1:317141/149‑1 (MQ=255) tCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGatc < 2:19238/149‑1 (MQ=255) tCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGc > 4:40247/1‑99 (MQ=255) tCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGc < 3:40247/99‑1 (MQ=255) cgcgCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCg > 1:175765/1‑149 (MQ=255) gcgcTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCCAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCgt > 2:242689/1‑149 (MQ=255) ctacAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTg < 8:128606/148‑1 (MQ=255) acAGCGACGCCCAGAACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGAACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGatcatc < 5:218116/137‑1 (MQ=255) cggccAGTACCTTCTCATCATCCGGGTCATCCGGATGCTGCGGATATTCCGTGTAGTCAATGTCTGCACGTTCCTCAAGCAGGCCCATTACCTTATGGAGGCGCTGCGCGGAAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTAt < 7:273454/146‑1 (MQ=255) cAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGAc > 6:163710/1‑149 (MQ=255) ccTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTg < 5:42464/148‑1 (MQ=255) cTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATgg > 6:78095/1‑88 (MQ=255) cTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATgg < 5:78095/88‑1 (MQ=255) cTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTg > 3:202695/1‑147 (MQ=255) cTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTg < 4:202695/147‑1 (MQ=255) cTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAAc > 5:160216/1‑149 (MQ=255) gcagcatcCGGATGACCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACg > 2:267642/5‑149 (MQ=255) catcCGGATGACCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGCGACGGTATCGACGCTGGTAACGgtgt > 8:10666/1‑149 (MQ=255) cGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTCg < 6:160216/148‑1 (MQ=255) gggTCATCCGGATGCTTCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAATCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTCGGc < 3:205961/149‑1 (MQ=255) | CGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTCGGC > NC_002947/4895755‑4896043 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |