Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,895,901 T→C 13.5% I119T (ATC→ACC)  PP_4304 → VIC family cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,895,9010TC13.5% 140.0 / 13.5 59I119T (ATC→ACC) PP_4304VIC family cation transporter
Reads supporting (aligned to +/- strand):  ref base T (25/26);  new base C (4/4);  total (29/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01

CGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTCGGCA  >  NC_002947/4895755‑4896044
                                                                                                                                                  |                                                                                                                                               
cGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACATCGACGCCCAGTACATGCTCATCATCCGGGTCATc                                                                                                                                                >  4:1246/1‑148 (MQ=255)
cGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATcc                                                                                                                                               <  4:89924/149‑1 (MQ=255)
      cATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGAtga                                                                                                                                         <  1:292904/149‑2 (MQ=255)
      cATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGAtga                                                                                                                                         <  6:44151/149‑2 (MQ=255)
         cACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGCTCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtgctgc                                                                                                                                      >  1:252182/1‑149 (MQ=255)
          aCCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtgctgc                                                                                                                                      <  4:143945/148‑1 (MQ=255)
                cTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCGTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGAGCTGTACTACAGCGACGCCCAGTACCTGCTAATCATCAGGGTCATCAGGATGCTGCGGATCt                                                                                                                                >  8:114698/1‑148 (MQ=255)
                   tACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGa                                                                                                                                   <  6:85416/142‑1 (MQ=255)
                   tACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGa                                                                                                                                   >  5:85416/1‑142 (MQ=255)
                   tACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTcc                                                                                                                             <  6:293268/148‑1 (MQ=255)
                   tACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTcc                                                                                                                             <  5:193420/148‑1 (MQ=255)
                     cTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgtg                                                                                                                          >  7:128606/1‑149 (MQ=255)
                     cTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt                                                                                                                           <  4:219763/148‑1 (MQ=255)
                     cTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt                                                                                                                           <  8:53037/148‑1 (MQ=255)
                      tGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGatgatga                                                                                                                                      >  3:130208/1‑135 (MQ=255)
                      tGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGatgatga                                                                                                                                      <  4:130208/136‑2 (MQ=255)
                      tGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCAACCGGGTCATCCGGATGCTGCGGATCTTCCgtg                                                                                                                          >  4:173842/1‑148 (MQ=255)
                             ccAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAg                                                                                                                  >  4:10840/1‑149 (MQ=255)
                               aaGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCt                                                                                                                <  2:273999/149‑1 (MQ=255)
                               aaGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCt                                                                                                                >  4:4960/1‑149 (MQ=255)
                                 gCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtg                                                                                                                                          <  4:317968/121‑1 (MQ=255)
                                 gCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGAtg                                                                                                                                          >  3:317968/1‑121 (MQ=255)
                                        cgcTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt                                                                                                                           >  2:254348/1‑129 (MQ=255)
                                        cgcTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCgt                                                                                                                           <  1:254348/129‑1 (MQ=255)
                                              gCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGAtga                                                                                                                                         >  3:293665/1‑108 (MQ=255)
                                              gCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGATGACCCGGAtga                                                                                                                                         <  4:293665/109‑2 (MQ=255)
                                                                cTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGAt                                                                               <  3:173842/149‑1 (MQ=255)
                                                                   gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGc                                                                                           <  4:309690/134‑1 (MQ=255)
                                                                   gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGc                                                                                           >  3:309690/1‑134 (MQ=255)
                                                                   gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATg                                                                              >  2:317141/1‑147 (MQ=255)
                                                                   gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATg                                                                              >  8:213613/1‑147 (MQ=255)
                                                                   gTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATg                                                                              >  6:42464/1‑147 (MQ=255)
                                                                       aTTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGgcg                                                                        >  1:19238/1‑149 (MQ=255)
                                                                               gCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTgcgc                                                                  <  7:213613/147‑1 (MQ=255)
                                                                               gCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGAGCTGCGCgg                                                                >  3:317279/1‑149 (MQ=255)
                                                                                 gATCCTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCgg                                                                >  6:333000/1‑147 (MQ=255)
                                                                                        ccGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTGATCATCCGGGTCATCCGGTTGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGTCCCATTACCTGATGGAGGCGCTGCGCGGCagcaagc                                                        <  2:252182/148‑1 (MQ=255)
                                                                                            ggATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGTGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGACGCGCTGCGCGGCAGCAAGCag                                                      >  3:268322/1‑146 (MQ=255)
                                                                                            ggATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCagaag                                                   <  1:317141/149‑1 (MQ=255)
                                                                                               tCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGatc                                                <  2:19238/149‑1 (MQ=255)
                                                                                                  tCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGc                                                                                               >  4:40247/1‑99 (MQ=255)
                                                                                                  tCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGc                                                                                               <  3:40247/99‑1 (MQ=255)
                                                                                                   cgcgCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCg                                            >  1:175765/1‑149 (MQ=255)
                                                                                                    gcgcTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCCAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCgt                                           >  2:242689/1‑149 (MQ=255)
                                                                                                            ctacAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTg                                    <  8:128606/148‑1 (MQ=255)
                                                                                                              acAGCGACGCCCAGAACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGAACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGatcatc                                             <  5:218116/137‑1 (MQ=255)
                                                                                                                     cggccAGTACCTTCTCATCATCCGGGTCATCCGGATGCTGCGGATATTCCGTGTAGTCAATGTCTGCACGTTCCTCAAGCAGGCCCATTACCTTATGGAGGCGCTGCGCGGAAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTAt                          <  7:273454/146‑1 (MQ=255)
                                                                                                                         cAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGAc                      >  6:163710/1‑149 (MQ=255)
                                                                                                                              ccTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTg                  <  5:42464/148‑1 (MQ=255)
                                                                                                                               cTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATgg                                                                             <  5:78095/88‑1 (MQ=255)
                                                                                                                               cTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATgg                                                                             >  6:78095/1‑88 (MQ=255)
                                                                                                                               cTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTg                  >  3:202695/1‑147 (MQ=255)
                                                                                                                               cTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTg                  <  4:202695/147‑1 (MQ=255)
                                                                                                                                  cTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAAc             >  5:160216/1‑149 (MQ=255)
                                                                                                                                   gcagcatcCGGATGACCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACg            >  2:267642/5‑149 (MQ=255)
                                                                                                                                       catcCGGATGACCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGCGACGGTATCGACGCTGGTAACGgtgt        >  8:10666/1‑149 (MQ=255)
                                                                                                                                           cGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTCg     <  6:160216/148‑1 (MQ=255)
                                                                                                                                            gggTCATCCGGATGCTTCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAATCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTCGGc   <  3:205961/149‑1 (MQ=255)
                                                                                                                                             tttCATACGGATGATTAGGATCTTCGGTTTACTCAAGCTCAGCCAGTACCTCAAGCAGGCCCATTACCTTATTGAGGCGCTGCGCGGCAGCAATCAGAATATCATACTGATCCTTGTGACGGTATCGACGCTGGTAACTGTGTTCGGCa  <  7:114698/147‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CGAATACATCACCCGCCTGTACTGTTCGCCCAAGCCGCTGCGCTATGCCTTCAGTTTCTACGGCCTGGTCGATTTACTGGCGATCGTGCCGGGGATCCTCGCGCTGTACTACAGCGACGCCCAGTACCTGCTCATCATCCGGGTCATCCGGATGCTGCGGATCTTCCGTGTACTCAAGCTCAGCCCGTACCTCAAGCAGGCCCATTACCTGATGGAGGCGCTGCGCGGCAGCAAGCAGAAGATCATCGTGTTCCTGGTGACGGTATCGACGCTGGTAACGGTGTTCGGCA  >  NC_002947/4895755‑4896044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑