Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,950,972 T→G 12.1% intergenic (‑122/+35) fliL ← / ← fliK flagellar protein FliL/flagellar hook‑length control protein FliK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,950,9720TG12.1% 90.9 / 3.4 39intergenic (‑122/+35)fliL/fliKflagellar protein FliL/flagellar hook‑length control protein FliK
Reads supporting (aligned to +/- strand):  ref base T (16/18);  new base G (3/2);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

TGACTGCTTCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCCGCCAC  >  NC_002947/4950829‑4951114
                                                                                                                                               |                                                                                                                                              
tGACTGCTTCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGcc                                                                                                                                            <  3:71215/148‑1 (MQ=255)
    tGCTTCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCTGTGCTGGCTTCTTCGCGGGCTCGCCcgcg                                                                                                                                        >  2:290529/1‑148 (MQ=255)
    tGCTTCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCcgcg                                                                                                                                        >  4:85751/1‑148 (MQ=255)
      cTTCTCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCgaa                                                                                                                                      <  2:148112/148‑1 (MQ=255)
       ttCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCTCGGGCTAGCCCGGgaag                                                                                                                                     >  7:98599/1‑148 (MQ=255)
         cGCTCTTCGCCATGCCAATAATCCGTCCTCCCTCGGGGGTTTAAATTATTATGGCTTGGCATTATCAATTGTTATTCAATATTTTTCATTGTATTTATTCCATCAGGTTTTGCTGTGCTGGCTTCTTCGCGGGCGAGCCCGCgaagaa                                                                                                                                   <  6:306870/148‑1 (MQ=255)
          gCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGTCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGaagaag                                                                                                                                  <  6:9692/148‑1 (MQ=255)
          gCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCgaagaa                                                                                                                                   <  3:73753/147‑1 (MQ=255)
                        cAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAagc                                                                                                                                 >  1:56717/1‑135 (MQ=255)
                        cAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAagc                                                                                                                                 <  2:56717/135‑1 (MQ=255)
                         aataatCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTAGCCCGCCAAGAAGCCCGCCCAGGCCAACa                                                                                                                  >  8:281924/1‑149 (MQ=255)
                          ataagCCGTCGTCCATCGGGGGTTCAAATGAGACTGGCTTGGCTTGAGCAATTGTTATGCCAGATTTTGCAGGGGAGGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAg                                                                                                                 <  8:98599/149‑1 (MQ=255)
                                       cATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCgta                                                                                                     <  6:90502/148‑1 (MQ=255)
                                            ggggTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATc                                                                                               <  7:160131/149‑1 (MQ=255)
                                                  cAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCTCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCg                                                                                          <  1:248105/148‑1 (MQ=255)
                                                   aaaGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCa                                                                                        >  7:43650/1‑149 (MQ=255)
                                                         gaATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCg                                                                                  >  4:236787/1‑149 (MQ=255)
                                                                       gAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACctg                                                                    >  8:61082/1‑149 (MQ=255)
                                                                       gAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACct                                                                     <  3:236787/148‑1 (MQ=255)
                                                                        aGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACctg                                                                    <  3:85751/148‑1 (MQ=255)
                                                                         gCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGAcc                                                                                           <  8:178221/124‑1 (MQ=255)
                                                                         gCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGAcc                                                                                           >  7:178221/1‑124 (MQ=255)
                                                                              tgtTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGAc                                                                                            <  8:56221/118‑1 (MQ=255)
                                                                              tgtTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGAc                                                                                            >  7:56221/1‑118 (MQ=255)
                                                                                      cAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAagc                                                                                                                                 >  2:181189/1‑73 (MQ=255)
                                                                                      cAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCGCGCCCGCGAAGAagc                                                                                                                                 <  1:181189/73‑1 (MQ=255)
                                                                                       aGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCgg                                                    <  8:43650/149‑1 (MQ=255)
                                                                                             tGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTa                                              >  1:10912/1‑149 (MQ=255)
                                                                                               cAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTTGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATc                                            <  1:290529/149‑1 (MQ=255)
                                                                                                                 agGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCcccccc                          >  5:290276/1‑149 (MQ=255)
                                                                                                                           gTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCCCCTGCTGCTCCAACGGCCGGGCGCTATCGACCGCTTCGGCCCCGCCCTGCGCGGcc                >  1:280599/1‑149 (MQ=255)
                                                                                                                               tGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGgc            >  1:124300/1‑149 (MQ=255)
                                                                                                                                 gCTTCTTCCCGGGCTGGCCGGGGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGgc            <  7:281924/147‑1 (MQ=255)
                                                                                                                                   ttcttcGCGGGCGAGCCGGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTTATCGACCGCACTGTCGCCGACTACCACCTGCTGCTCCTAAGGCCGGGCGCTATCGACCGCTTCGGCGCCACCCTGCTCGGCCCGgcgcgg        >  6:113161/1‑148 (MQ=255)
                                                                                                                                   ttcttcGCGGGCGAGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGgcgcgc        >  1:292547/1‑149 (MQ=255)
                                                                                                                                     cttcGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGcgcc       <  6:117756/148‑1 (MQ=255)
                                                                                                                                         gcgGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGgcgc          <  4:135990/141‑1 (MQ=255)
                                                                                                                                         gcgGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGgcgc          >  3:135990/1‑141 (MQ=255)
                                                                                                                                         gcgGGCGAGCCCGCGAAGAAGCCAGCACAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCCGCCAc  >  7:56761/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                              
TGACTGCTTCGCTCTTCGCCATGCCAATAATCCGTCGTCCATCGGGGGTTCAAAGGAGAATGGCTTGGCTTGAGCAAGTGTTATGCCAGATTTTGCAGGGGATGGATTCCAGCAGGTTTTGCGGTGCTGGCTTCTTCGCGGGCTCGCCCGCGAAGAAGCCAGCCCAGGCCAACAGTTATCAGGCGTAGTAATCGACCGCACTGTCGCCGACAACCACCTGCTGCTCCAAAGGCCGGGCGCTATCGACCGCTTCGGCCCCACCCTGCTCGGCCCGGCGCGCCGCCAC  >  NC_002947/4950829‑4951114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑