Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,988,365 G→C 13.0% intergenic (+35/+59) ycgR → / ← PP_4398 flagellar brake protein YcgR/MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,988,3650GC13.0% 121.8 / 10.2 46intergenic (+35/+59)ycgR/PP_4398flagellar brake protein YcgR/MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (18/22);  new base C (2/4);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TTTGCCGGCGTGCGTTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCAGCCGCCACCGGC  >  NC_002947/4988220‑4988505
                                                                                                                                                  |                                                                                                                                            
tttGCCGGCGTGCGTTTCCACAGAATGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAgg                                                                                                                                           >  7:49031/1‑149 (MQ=255)
    ccGGCGTGCGTTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGc                                                                                                                                       <  1:27305/149‑1 (MQ=255)
        cgtgcgtTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTCAGCAGGTGGCGttt                                                                                                                                   <  8:210219/149‑1 (MQ=255)
         gtgcgtTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGtttt                                                                                                                                  >  1:297974/1‑149 (MQ=255)
          tgcgtTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTCAGCAGGTGGCGTTTTc                                                                                                                                 <  4:282883/149‑1 (MQ=255)
          tgcgtTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTCAGCAGGTGGCGTTTTc                                                                                                                                 <  6:88252/149‑1 (MQ=255)
            cgtTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCAt                                                                                                                               <  4:233651/149‑1 (MQ=255)
              tttCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCAttt                                                                                                                             <  1:235996/149‑1 (MQ=255)
              tttCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCAttt                                                                                                                             <  2:44673/149‑1 (MQ=255)
               ttCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTtg                                                                                                                            >  3:142419/1‑149 (MQ=255)
                 ccACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTtgt                                                                                                                           <  4:142419/148‑1 (MQ=255)
                       ccTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTctg                                                                                                                    >  1:295273/1‑149 (MQ=255)
                        cTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTctgc                                                                                                                   <  6:42264/149‑1 (MQ=255)
                         tGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTctgct                                                                                                                  >  5:60888/1‑149 (MQ=255)
                                             aaGATCGAGATTTTCGTGTACCAGCTGCAGCGTGAGGCGCTGCGGTTCGACAAGGA‑CGATTAATGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGt                                                                                              <  4:226467/149‑1 (MQ=255)
                                             aaGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCggg                                                                                               >  4:215281/1‑148 (MQ=255)
                                                 tCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAAc                                                                                          >  7:276568/1‑149 (MQ=255)
                                                    agagTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTgctgggc                                                                                                              >  7:16049/1‑126 (MQ=255)
                                                    agagTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTgctgggc                                                                                                              <  8:16049/126‑1 (MQ=255)
                                                     gagTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGc                                                                                      <  6:60888/149‑1 (MQ=255)
                                                              gtACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTgctgggct                                                                                                             >  6:206477/1‑117 (MQ=255)
                                                              gtACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTgctgggct                                                                                                             <  5:206477/117‑1 (MQ=255)
                                                               tACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACAcc                                                                             >  3:124605/1‑148 (MQ=255)
                                                                 ccAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGAc                                                                          >  2:74064/1‑149 (MQ=255)
                                                                  cAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGAGCGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGAc                                                                          >  1:215772/1‑149 (MQ=255)
                                                                    gCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAACCCCGCTCCTACACCGACTTg                                                                       >  1:96758/1‑149 (MQ=255)
                                                                       gCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTcgc                                                                                                  <  8:296056/119‑1 (MQ=255)
                                                                          gCGTGAGGCGCGGCGGTTCGACAAAGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGAcc                                                                 >  5:44683/1‑149 (MQ=255)
                                                                            gTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACctc                                                               <  8:49031/149‑1 (MQ=255)
                                                                               aGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTc                                                            <  4:124605/149‑1 (MQ=255)
                                                                                   gcggcgGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGc                                                                                      <  5:247145/119‑1 (MQ=255)
                                                                                   gcggcgGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGc                                                                                      >  6:247145/1‑119 (MQ=255)
                                                                                       cggTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCt                                                    <  3:215281/149‑1 (MQ=255)
                                                                                             gACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGAGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTtgct                                              >  3:210660/1‑149 (MQ=255)
                                                                                                aaGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGATTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTgcg                                           >  2:174068/1‑149 (MQ=255)
                                                                                                 aagA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTgcgc                                          <  6:44683/147‑1 (MQ=255)
                                                                                                  ggA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTg                                                   <  8:127817/139‑1 (MQ=255)
                                                                                                  ggA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGAGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTg                                                   >  7:127817/1‑139 (MQ=255)
                                                                                                      cGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGccc                                      <  1:74064/149‑1 (MQ=255)
                                                                                                            ttGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTtctc                                 <  1:174068/148‑1 (MQ=255)
                                                                                                               aTCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTc                              <  4:118422/148‑1 (MQ=255)
                                                                                                                  gtcggtcgGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATc                           <  2:297974/148‑1 (MQ=255)
                                                                                                                     ggtcggtAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCg                       <  8:276568/149‑1 (MQ=255)
                                                                                                                                  gAAAACGCCACCTCAGCAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGCAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCAgccg          >  1:92744/1‑149 (MQ=255)
                                                                                                                                    aaaCGCCACCTCAGCAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTc               >  6:952/1‑142 (MQ=255)
                                                                                                                                    aaaCGCCACCTCAGCAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTc               <  5:952/142‑1 (MQ=255)
                                                                                                                                          ctgctgctgATGTGTCGTTTTCATTTGTGTTTCTGCTGGGCTTGCCGCTTCGCGGGTAAACCCGCTCCTACAGCGACTTGCTGACCTCTTCAGGCGCGTGTTGCTGCGCGACCTTCTCTTCATCCTCGGTGTATTCAGCCGCCAGCgtc  <  2:96758/146‑3 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TTTGCCGGCGTGCGTTTCCACAACCTGAGCGGGCAGGCGCAGCGCAAGATCGAGAGTTTCGTGTACCAGCTGCAGCGTGAGGCGCGGCGGTTCGACAAGGA‑CGATTATTGATCGTCGGTCGGTAAAACAGAAAACGCCACCTGCTGAGGTGGCGTTTTCATTTGTGTTTCTGCTGGGCTGGCCGCTTCGCGGGTAAACCCGCTCCTACACCGACTTGCTGACCTCTTCAGGCGCCTGTTGCTGCGCGCCCTTCTCTTCATCCTCGGTGTCTTCAGCCGCCACCGGC  >  NC_002947/4988220‑4988505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑