| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 4,740,811 | (T)7→6 | intergenic (+48/+705) | gltA → / ← yeiW | citrate synthase/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,740,805 | 0 | T | . | 90.9% | 156.6 / 14.2 | 45 | intergenic (+42/+711) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (0/4); new base . (21/19); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTT > NC_002947/4740660‑4740946 | aCTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCTAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCtttt < 5:72953/149‑1 (MQ=255) cTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCttttt < 3:319324/149‑1 (MQ=255) tGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCGCGGGCAGCGgtttt > 8:75438/1‑148 (MQ=255) gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCttttttat > 2:124969/1‑147 (MQ=255) gaaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGCCAGCCttttttat < 3:35494/149‑3 (MQ=255) aaagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCttttttatt < 8:177582/149‑4 (MQ=255) aagaaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCCGCGAGCAGAAGAGCGACATCGTAGCCCTCAAAGACCGCAAGTAAGATGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCACGGGCAGCCgtttttattt > 3:288298/1‑145 (MQ=255) aaaTGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCttttttatttgtc < 5:374838/149‑8 (MQ=255) tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCtttttta < 2:276997/135‑2 (MQ=255) tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCtttttta > 1:276997/1‑134 (MQ=255) cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg > 1:52614/1‑149 (MQ=255) aaGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg < 6:131067/149‑1 (MQ=255) aaGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCgg > 1:159215/1‑149 (MQ=255) aaGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TATATTATTTGTCCTGTGCCGGCCTCTTCGCgg > 1:92064/1‑149 (MQ=255) gATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCggg > 8:156706/1‑147 (MQ=255) gATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc < 3:204815/149‑1 (MQ=255) tCGGCCGTCCGCGCCAGTT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt < 3:367300/149‑1 (MQ=255) tCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt < 2:54281/149‑1 (MQ=255) tCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt > 1:285374/1‑149 (MQ=255) tCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCGGCCtt > 2:301981/1‑149 (MQ=255) cgcgCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa < 2:245822/149‑1 (MQ=255) gcgcCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAa‑g < 6:342682/149‑1 (MQ=255) gcgcCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCCGCCTTTCCcgcg > 4:186440/1‑146 (MQ=255) cgcCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAa‑ga < 7:156706/149‑1 (MQ=38) gcCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAa‑gag < 6:235541/149‑1 (MQ=255) cAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGATGGCT‑G‑CCCTCGGGCAG‑CGTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGc < 4:288298/149‑1 (MQ=255) cAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGc < 7:75438/149‑1 (MQ=255) aGCT‑GTACTCCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGcc < 1:1316/149‑1 (MQ=255) cT‑GTACACCGGCGAGCAGAAGCGTGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCgg < 7:285485/149‑1 (MQ=255) aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGCCACCACaaaa > 8:36768/1‑149 (MQ=255) taggcccaCATTGTTGCCCTTAAAGACCCCAATTAATCTTCCTTACCCCAACCCAATCTGGCG‑G‑CCCGGGGGCATTTTTTTTTTTTTGGCCTGG‑GCCGGCCTTTTCGCGGCCTTGCCCGCTAATCTGGCCGGCACAGACACCACAAAAc < 2:159215/142‑1 (MQ=255) gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTAGACCATCGGGCAGCC‑TTTTTTATTTTTCATGTGCCGGCCTATTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAAc > 1:110466/1‑148 (MQ=255) gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCCCAGCGACCACAAAAGCt > 6:371592/1‑148 (MQ=255) gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTc < 2:52614/149‑1 (MQ=255) aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAa‑ga < 5:362828/116‑1 (MQ=255) aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAa‑ga > 6:362828/1‑116 (MQ=255) aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTAGCGGCCTTTCCCGCCCA‑GAGGCCGGCCCACACACCACAACACCAACGttttt > 3:20864/1‑149 (MQ=255) aaaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGTCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt > 6:170761/1‑148 (MQ=255) aaaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt < 7:244649/148‑1 (MQ=255) aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg > 7:116037/1‑148 (MQ=255) cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCGTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTACGCCCGCTCACGccg > 1:122042/1‑147 (MQ=255) aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc > 4:283835/1‑149 (MQ=255) aGCTGCCGTAGCCCACCGCAAGACGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa < 2:122042/149‑1 (MQ=255) gCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCGTCGCGGCCGTGCCCCCGAC‑GAGGCCGGCCCCGCCCCCACAAAACCCCCGGGTTTCTCCGCCCGCGCACGCACCCCGGTCAg > 5:293468/1‑149 (MQ=255) gtGCAGTAGCCGAACGCATGAAGGCT‑G‑CCCTCGGGCA‑TCTTTTTTTATTTTTCCTGTCCCGGCCTCTTCGGGGCCTTGCCCGCTAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg < 3:282333/148‑1 (MQ=255) cTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGAAACAACCAAACAACAGTTTATCTACGCCCGCGCACGCAGCCCGTTCAgg > 1:283030/1‑149 (MQ=255) cAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa < 8:116037/149‑1 (MQ=255) ccGACCGCACGCAGGGG‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCGTGGCCGGCCGCTTCGCGGCGTTGCCCGCGAT‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc < 6:293468/149‑1 (MQ=255) aaCGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc < 2:285374/149‑1 (MQ=255) cGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcgc < 1:301981/149‑1 (MQ=255) aagaagGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGGCCGCGCA‑GAGGCCGGCACAGACCCCACAAAACCTCAGTTTTTCTCAGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc > 7:334130/1‑149 (MQ=255) gt‑G‑CCCGGGGGCAG‑CTTTTTTTTTTGGCCGTGTGCCGGCTTCTTCGCGGCTTTGCCGGCTAT‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACg < 4:20864/148‑1 (MQ=255) t‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACg > 5:285303/1‑148 (MQ=255) g‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACACCCACCACAAAACCTCAGTTTTTATCCCCCCGCTCCCGCCGCCCGTTCAGGGTTTTGAACCGCGCATCCACCACGaa > 2:241489/1‑149 (MQ=255) ggccagcTTTTTTTATTGGCCCGGGCCCGGCGTCTGCGCGGCCTGGCCCGCTAA‑GCGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt < 3:186440/143‑1 (MQ=255) | ACTGGAAAGAAATGCTCTCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTT > NC_002947/4740660‑4740946 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |