| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 97.6% | 140.6 / ‑3.5 | 42 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (22/19); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA > NC_002947/1932490‑1932774 | aTGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTg < 5:288381/149‑1 (MQ=255) tGGCGGCGTCTTCGAAGAGGAGCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑c < 5:19538/149‑1 (MQ=255) gTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑cccgcg > 2:59855/1‑143 (MQ=255) gACTGCGGCTTTGCTGGTCTCACTAAAAATGCAGTAACCGGTGGGGCCGCTTTGCGGCCGTTCGCGGGCATGCCCGCTCCTAC‑AATGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGt < 8:301803/149‑1 (MQ=25) gACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGt < 4:234836/149‑1 (MQ=255) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGGCACCCCCCGCCCACAAAAGGTAATGGGGCCCCGGTTGGGACGGGGTTG‑CCCCGAAAGGCGGCACAGGCCCCCCCaaaattg > 6:43244/1‑145 (MQ=255) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTc < 3:321273/149‑1 (MQ=255) aGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGa > 6:270647/1‑149 (MQ=255) ccgaactcACGAAAATCCCAGTAAACGCGGGGGCCGCTTTGCGGCCCGTCGCGGGCAACCCCGCTCCTCC‑AAAGGATATGCGCCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGAt < 4:99269/144‑1 (MQ=37) aGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGAt > 7:248206/1‑148 (MQ=255) gTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGGA‑AGGCCGCCCCCAATTGTCGACTACGCGGATCAg > 7:334919/1‑149 (MQ=38) cctcACCAAAATAGCATTAAACGCGGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTAC‑AAAGTATATGCGTCCCCTGTAGGAGGGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGc < 8:335008/148‑1 (MQ=38) cACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAt > 2:259407/1‑149 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGAt > 3:99269/1‑141 (MQ=255) cGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATcc < 2:58078/149‑1 (MQ=255) aGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGGCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGAtgc > 1:136528/1‑140 (MQ=255) tAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGAt < 1:112654/148‑1 (MQ=255) aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGt < 1:259407/148‑1 (MQ=255) gggCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑cccg < 1:352392/77‑3 (MQ=255) ccgcaaagCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCGGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAg > 5:163995/8‑148 (MQ=38) cTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 2:81121/149‑1 (MQ=255) tttGCGGCCCTTCGCGGGCAAGCCCCCTGCTAC‑AAAGGATATGGGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCCGCCCCAAAATGTCGACTACGCGGATCAGCGCTGCGGCAGGGGGATGTTCAGCTGCACAATCg > 8:4843/1‑149 (MQ=37) gCGGCCCTGCGCGTCCAACCCCGCTCCTAC‑ACAGTATCTGCTTCCCCTGAAGCAGCTGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag < 4:301424/148‑1 (MQ=255) ggCCCTTCGCGGGCAAGCCCGCTCCTTC‑AACGGAAAGGCGGCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcag < 6:163995/149‑1 (MQ=38) gCAAGCCCGCGCCTCC‑AAAGGATATGCGGCCCCTTTAGGCGCGGGCTGGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt < 8:334919/149‑1 (MQ=255) agatctcGCTCCTAC‑AAAGGTTATGCGTCCCCTGTAGGAGCGGGCTTGCCCTCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGATCCAGAATCGAGCAGCTGCCCTGGtttt < 6:215461/143‑1 (MQ=39) aaGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc > 1:370860/1‑149 (MQ=255) gCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc > 2:59273/1‑147 (MQ=255) gCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGCAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt > 5:215461/1‑142 (MQ=255) cTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGa < 1:43694/147‑1 (MQ=255) c‑AAAGGATATGGGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc < 3:72652/149‑1 (MQ=255) c‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg > 5:349446/1‑148 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 7:161916/1‑149 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca > 6:131422/1‑149 (MQ=255) aGGATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGGTTACCAGCTCGATATGcac > 8:252780/1‑148 (MQ=255) gATATGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcat > 3:148750/1‑148 (MQ=255) atGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt > 7:23741/1‑133 (MQ=255) atGCGTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCt < 8:23741/133‑1 (MQ=255) gTCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg > 3:152539/1‑149 (MQ=255) tCCCCTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 4:148750/149‑1 (MQ=255) cccTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat < 2:88168/130‑1 (MQ=255) cccTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat > 1:88168/1‑130 (MQ=255) ccTGTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGa > 7:4088/1‑149 (MQ=255) gTAGGAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGt > 5:109700/1‑149 (MQ=255) ggAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 1:59855/148‑1 (MQ=255) ggAGCGGGCTTGCCCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCCGCACGGTCAAGTTCAGCTCCAGAATCGAGCAGCTGCCATGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGATca > 4:265297/1‑149 (MQ=38) | ATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAC‑AAAGGATATGCGTCCCCTGTAGGAGCGGGCTTG‑CCGCGAAGGGCTGCA‑AGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCA > NC_002947/1932490‑1932774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |