Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,921,460	G→A	G3494R (GGG→AGG)	PP_2561 →	hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,921,460	0	G	A	100.0%	104.4 / NA	33	G3494R (GGG→AGG)	PP_2561	hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/18);  total (15/18)

TGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC  >  NC_002947/2921320‑2921600                                                                                                                                             |                                                                                                                                              tGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGcc                                                                                                                                       >  3:276408/1‑149 (MQ=255)      ggcgcgcccGGTCACGACCACCTGAACGGGGGCGCTGTCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCaa                                                                                                                                  <  2:372192/142‑1 (MQ=255)        cgcggCAGGTAACGCCCACCGGCCCGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACg                                                                                                                                <  8:63754/146‑1 (MQ=255)                     gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACgg                                                                                                                   >  4:332902/1‑149 (MQ=255)                     gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCAGGCAACGACTTCCTCAACgg                                                                                                                   >  8:275865/1‑149 (MQ=255)                          ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                              >  7:361659/1‑149 (MQ=255)                              accggcggCCCAGGCGAAGACCGCCGTGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGGAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAgg                                                                                                          <  4:276408/147‑1 (MQ=255)                                   gggCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAAc                                                                                                      >  3:220288/1‑148 (MQ=255)                                    ggCGCTTGCGATGACAGCCTGGATGGCGGCAACGTCAATGACAACCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGa                                                                                                    <  8:361659/149‑1 (MQ=255)                                        cTGTCGATGACAGCCTGGATTGCGGCAACGGCAATTACACCCTGCAGGGCGTCGATGGTAACGACACATTTTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACg                                                                                                <  7:318652/149‑1 (MQ=255)                                         tGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACgg                                                                                               >  6:164388/1‑149 (MQ=255)                                           gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACgg                                                                                               <  3:268751/147‑1 (MQ=255)                                                  cAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑Tggtgggt                                                                                      <  5:164388/148‑1 (MQ=255)                                                     ccTGGATGGCGTCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTgggg                                                                                  <  6:230229/149‑1 (MQ=255)                                                     ccTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTgggg                                                                                  >  4:207633/1‑149 (MQ=255)                                                      cTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGTTGGGTGGGGc                                                                                 >  3:217111/1‑149 (MQ=255)                                                               ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAAc                                                                          >  1:79214/1‑147 (MQ=255)                                                               ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAAc                                                                          <  2:79214/147‑1 (MQ=255)                                                                 caacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGaca                                                                      <  7:275865/149‑1 (MQ=255)                                                                   acggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGacac                                                                     >  7:20776/1‑148 (MQ=255)                                                                   acggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACAcc                                                                    <  8:20776/149‑1 (MQ=255)                                                                          tGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCg                                                             >  5:33623/1‑149 (MQ=255)                                                                               cccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAcc                                                        >  2:303178/1‑149 (MQ=255)                                                                                              aTGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAtgtg                                         <  3:332902/149‑1 (MQ=255)                                                                                                      gACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGtt                                 >  7:258576/1‑149 (MQ=255)                                                                                                       acacATTGTTCGGCGTAGTGGGTACCGACACCCTGATCAGGGGGGCCGGGAACGAATTCCTCAACGGTGCGGCAGGTAACGATACGGCTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGtt                                 <  1:303178/149‑1 (MQ=255)                                                                                                        cacaTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCg                               <  6:33623/149‑1 (MQ=255)                                                                                                              gTTCGGCGAAGTTGGTACCTACACCCTGATCAGGGGTGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCa                          <  4:217111/148‑1 (MQ=255)                                                                                                                   gCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCttt                    <  3:207633/149‑1 (MQ=255)                                                                                                                                cGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGa       >  6:111324/1‑149 (MQ=255)                                                                                                                                 gACACCCTGATCAGGGGGGCCTGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGCGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGat      <  5:333314/149‑1 (MQ=255)                                                                                                                                   gacCCTGATCAGGGGGGGCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACTATACGG‑TGGTGGGTTGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatca    <  4:220288/148‑1 (MQ=255)                                                                                                                                      ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc  >  7:270077/1‑148 (MQ=255)                                                                                                                                             |                                                                                                                                              TGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGG‑TGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC  >  NC_002947/2921320‑2921600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑