Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,740,816 T→G intergenic (+53/+700) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,740,8160TG95.7% 151.4 / ‑6.7 47intergenic (+53/+700)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base G (21/24);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA  >  NC_002947/4740679‑4740954
                                                                                                                                            |                                                                                                                                           
cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCgg                                                                                                                                  >  1:52614/1‑149 (MQ=255)
          aaGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                                       <  6:131067/149‑1 (MQ=255)
          aaGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCgg                                                                                                                       >  1:159215/1‑149 (MQ=255)
          aaGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TATATTATTTGTCCTGTGCCGGCCTCTTCGCgg                                                                                                                       >  1:92064/1‑149 (MQ=255)
            gATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCggg                                                                                                                      >  8:156706/1‑147 (MQ=255)
            gATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                                     <  3:204815/149‑1 (MQ=255)
              tCGGCCGTCCGCGCCAGTT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                                   <  3:367300/149‑1 (MQ=255)
              tCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                                   >  1:285374/1‑149 (MQ=255)
              tCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCtt                                                                                                                   <  2:54281/149‑1 (MQ=255)
              tCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCGGCCtt                                                                                                                   >  2:301981/1‑149 (MQ=255)
                       cgcgCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                          <  2:245822/149‑1 (MQ=255)
                        gcgcCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAa‑g                                                                                                        <  6:342682/149‑1 (MQ=255)
                        gcgcCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCCGCCTTTCCcgcg                                                                                                            >  4:186440/1‑146 (MQ=255)
                         cgcCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGGCAAGGCCGCGAa‑ga                                                                                                       <  7:156706/149‑1 (MQ=38)
                          gcCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAa‑gag                                                                                                      <  6:235541/149‑1 (MQ=255)
                            cAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGATGGCT‑G‑CCCTCGGGCAG‑CGTTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGc                                                                                                    <  4:288298/149‑1 (MQ=255)
                            cAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGc                                                                                                    <  7:75438/149‑1 (MQ=255)
                             aGCT‑GTACTCCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGcc                                                                                                   <  1:1316/149‑1 (MQ=255)
                               cT‑GTACACCGGCGAGCAGAAGCGTGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCgg                                                                                                 <  7:285485/149‑1 (MQ=255)
                                                aGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGCCACCACaaaa                                                                                 >  8:36768/1‑149 (MQ=255)
                                                  taggcccaCATTGTTGCCCTTAAAGACCCCAATTAATCTTCCTTACCCCAACCCAATCTGGCG‑G‑CCCGGGGGCATTTTTTTTTTTTTGGCCTGG‑GCCGGCCTTTTCGCGGCCTTGCCCGCTAATCTGGCCGGCACAGACACCACAAAAc                                                                                <  2:159215/142‑1 (MQ=255)
                                                    gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTAGACCATCGGGCAGCC‑TTTTTTATTTTTCATGTGCCGGCCTATTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAAc                                                                                >  1:110466/1‑148 (MQ=255)
                                                    gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCCCAGCGACCACAAAAGCt                                                                              >  6:371592/1‑148 (MQ=255)
                                                    gcgcGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTc                                                                             <  2:52614/149‑1 (MQ=255)
                                                           aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAa‑ga                                                                                                       <  5:362828/116‑1 (MQ=255)
                                                           aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAa‑ga                                                                                                       >  6:362828/1‑116 (MQ=255)
                                                           aTCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTAGCGGCCTTTCCCGCCCA‑GAGGCCGGCCCACACACCACAACACCAACGttttt                                                                      >  3:20864/1‑149 (MQ=255)
                                                                       aaaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGTCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                           >  6:170761/1‑148 (MQ=255)
                                                                       aaaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCt                                                           <  7:244649/148‑1 (MQ=255)
                                                                           aCCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                       >  7:116037/1‑148 (MQ=255)
                                                                             cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCGTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTACGCCCGCTCACGccg                                                    >  1:122042/1‑147 (MQ=255)
                                                                                aaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGccc                                                 >  4:283835/1‑149 (MQ=255)
                                                                                     aGCTGCCGTAGCCCACCGCAAGACGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa                                            <  2:122042/149‑1 (MQ=255)
                                                                                      gCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCGTCGCGGCCGTGCCCCCGAC‑GAGGCCGGCCCCGCCCCCACAAAACCCCCGGGTTTCTCCGCCCGCGCACGCACCCCGGTCAg                                           >  5:293468/1‑149 (MQ=255)
                                                                                       gtGCAGTAGCCGAACGCATGAAGGCT‑G‑CCCTCGGGCA‑TCTTTTTTTATTTTTCCTGTCCCGGCCTCTTCGGGGCCTTGCCCGCTAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                          <  3:282333/148‑1 (MQ=255)
                                                                                       cTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGAAACAACCAAACAACAGTTTATCTACGCCCGCGCACGCAGCCCGTTCAgg                                          >  1:283030/1‑149 (MQ=255)
                                                                                          cAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                                       <  8:116037/149‑1 (MQ=255)
                                                                                                ccGACCGCACGCAGGGG‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCGTGGCCGGCCGCTTCGCGGCGTTGCCCGCGAT‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAAc                                 <  6:293468/149‑1 (MQ=255)
                                                                                                   aaCGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgc                              <  2:285374/149‑1 (MQ=255)
                                                                                                     cGCAAGAAGGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGgcgc                            <  1:301981/149‑1 (MQ=255)
                                                                                                        aagaagGCT‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGGCCGCGCA‑GAGGCCGGCACAGACCCCACAAAACCTCAGTTTTTCTCAGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATc                         >  7:334130/1‑149 (MQ=255)
                                                                                                               gt‑G‑CCCGGGGGCAG‑CTTTTTTTTTTGGCCGTGTGCCGGCTTCTTCGCGGCTTTGCCGGCTAT‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACg                  <  4:20864/148‑1 (MQ=255)
                                                                                                                t‑G‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACg                  >  5:285303/1‑148 (MQ=255)
                                                                                                                  g‑CCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACACCCACCACAAAACCTCAGTTTTTATCCCCCCGCTCCCGCCGCCCGTTCAGGGTTTTGAACCGCGCATCCACCACGaa                >  2:241489/1‑149 (MQ=255)
                                                                                                                          ggccagcTTTTTTTATTGGCCCGGGCCCGGCGTCTGCGCGGCCTGGCCCGCTAA‑GCGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt          <  3:186440/143‑1 (MQ=255)
                                                                                                                                  ttttttATTTGTCCTGTGCCGGCCTCTTAGCGGCCTTGCCCGCGAA‑GAGGCCGGGACAGACACCACAAAACCTGAGTTTTTATACGCCCGCTGACGCAGCCCTTTGAg                                           >  3:188972/1‑108 (MQ=25)
                                                                                                                                    ttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGTCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccacca  <  5:170761/147‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                           
CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCT‑GTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCT‑G‑CCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAA‑GAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACCA  >  NC_002947/4740679‑4740954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑