Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 4,741,234 C→T intergenic (+471/+282) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,741,2340CT97.6% 134.2 / ‑4.4 42intergenic (+471/+282)gltA/yeiWcitrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (15/26);  total (16/26)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACC‑TCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAC  >  NC_002947/4741124‑4741364
                                                                                                               |                                                                                                                                   
cGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGTCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCg                                                                                                <  6:215416/148‑1 (MQ=255)
   ctcctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCggaca                                                                                            <  4:150925/149‑5 (MQ=255)
      ctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCggacacgt                                                                                         >  5:378421/1‑142 (MQ=255)
             gTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctg                                                                                  <  2:150937/149‑3 (MQ=255)
               cGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctg                                                                                  >  1:155330/1‑145 (MQ=255)
                gTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctgca                                                                                <  6:282531/148‑5 (MQ=255)
                  aaTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcg                                                                             >  1:380159/1‑148 (MQ=255)
                    tgtgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcc                                                                           <  5:274975/149‑1 (MQ=255)
                      tgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcctc                                                                         <  6:171999/149‑3 (MQ=255)
                           caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctgcag                                                                               >  5:231595/1‑133 (MQ=255)
                           caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctgcag                                                                               <  6:231595/138‑6 (MQ=255)
                                  tCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcct                                                                                   >  5:282689/1‑126 (MQ=255)
                                  tCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcct                                                                                   <  6:282689/127‑2 (MQ=255)
                                    aCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcg                                                           >  2:268438/1‑149 (MQ=255)
                                      gcggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggc                                                         <  8:201559/149‑1 (MQ=255)
                                          cgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                     <  6:106522/149‑1 (MQ=255)
                                              aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                 <  2:129955/149‑1 (MQ=255)
                                                  gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                             <  8:184995/149‑1 (MQ=255)
                                                  gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                             <  2:380159/149‑1 (MQ=255)
                                                    cATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                           <  6:378421/149‑1 (MQ=255)
                                                    cATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                           <  4:110816/149‑1 (MQ=255)
                                                          gTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGCTcaca                                       >  7:108433/1‑147 (MQ=255)
                                                               gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                 >  8:258308/1‑148 (MQ=255)
                                                                aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGCCACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                 >  5:47668/1‑147 (MQ=255)
                                                                  cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                             <  7:258308/149‑1 (MQ=255)
                                                                  cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCACCTTCTGCGACGTGC‑ATTCGAGAATCCAGGCACAGGCCCCGGACAAGTCCTCCTGCAGAGCCTCGCCCTTGGCCAGCGGCGCCTTGATAACGCTCACACCGCGATAgc                             >  7:154720/1‑148 (MQ=255)
                                                                  cTGGGCATAGGTCCAGCTCTGGTCACCTTCGAGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCCGCGCCTAGACCTTGGCCAGCGGCGCCTTGATCACGGTCAAACCGCGAAAgg                             >  4:310535/1‑149 (MQ=255)
                                                                     ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGTCTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                           <  2:256825/148‑1 (MQ=255)
                                                                     ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCTCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                            <  7:233050/147‑1 (MQ=255)
                                                                      gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                         <  8:58430/149‑1 (MQ=255)
                                                                      gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                         <  3:363295/149‑1 (MQ=255)
                                                                            gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                   <  1:268438/149‑1 (MQ=255)
                                                                             tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                               <  2:221360/136‑1 (MQ=255)
                                                                             tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                               >  1:221360/1‑136 (MQ=255)
                                                                                 gCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAcc              >  5:244254/1‑149 (MQ=255)
                                                                                       gTCACCTGCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGc          <  3:310535/147‑1 (MQ=255)
                                                                                          aCCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                         <  6:63580/113‑1 (MQ=255)
                                                                                          aCCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                         >  5:63580/1‑113 (MQ=255)
                                                                                          aCCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt     <  7:149675/149‑1 (MQ=255)
                                                                                             ttCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt     <  7:125853/146‑1 (MQ=255)
                                                                                             ttCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt     >  8:125853/1‑146 (MQ=255)
                                                                                             ttCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc  >  5:157814/1‑149 (MQ=255)
                                                                                                               |                                                                                                                                   
CGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACC‑TCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAC  >  NC_002947/4741124‑4741364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑