Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,741,255	C→T	intergenic (+492/+261)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,741,255	0	C	T	100.0%	152.0 / NA	45	intergenic (+492/+261)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/28);  total (17/28)

CGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACC‑TCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTC  >  NC_002947/4741124‑4741389                                                                                                                                     |                                                                                                                                        cGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGTCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCg                                                                                                                         <  6:215416/148‑1 (MQ=255)    ctcctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCggaca                                                                                                                     <  4:150925/149‑5 (MQ=255)       ctTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCggacacgt                                                                                                                  >  5:378421/1‑142 (MQ=255)              gTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctg                                                                                                           <  2:150937/149‑3 (MQ=255)                cGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctg                                                                                                           >  1:155330/1‑145 (MQ=255)                 gTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctgca                                                                                                         <  6:282531/148‑5 (MQ=255)                   aaTGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcg                                                                                                      >  1:380159/1‑148 (MQ=255)                     tgtgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcc                                                                                                    <  5:274975/149‑1 (MQ=255)                       tgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGcgcctc                                                                                                  <  6:171999/149‑3 (MQ=255)                            caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctgcag                                                                                                        <  6:231595/138‑6 (MQ=255)                            caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcctgcag                                                                                                        >  5:231595/1‑133 (MQ=255)                                   tCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcct                                                                                                            <  6:282689/127‑2 (MQ=255)                                   tCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGtcctcct                                                                                                            >  5:282689/1‑126 (MQ=255)                                     aCGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcg                                                                                    >  2:268438/1‑149 (MQ=255)                                       gcggcgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAgcggc                                                                                  <  8:201559/149‑1 (MQ=255)                                           cgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCt                                                                              <  6:106522/149‑1 (MQ=255)                                               aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGAt                                                                          <  2:129955/149‑1 (MQ=255)                                                   gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                      <  2:380159/149‑1 (MQ=255)                                                   gCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACg                                                                      <  8:184995/149‑1 (MQ=255)                                                     cATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                    <  4:110816/149‑1 (MQ=255)                                                     cATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGt                                                                    <  6:378421/149‑1 (MQ=255)                                                           gTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGCTcaca                                                                >  7:108433/1‑147 (MQ=255)                                                                gAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                          >  8:258308/1‑148 (MQ=255)                                                                 aaCTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGCCACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGa                                                          >  5:47668/1‑147 (MQ=255)                                                                   cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAgg                                                      <  7:258308/149‑1 (MQ=255)                                                                   cTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCACCTTCTGCGACGTGC‑ATTCGAGAATCCAGGCACAGGCCCCGGACAAGTCCTCCTGCAGAGCCTCGCCCTTGGCCAGCGGCGCCTTGATAACGCTCACACCGCGATAgc                                                      >  7:154720/1‑148 (MQ=255)                                                                   cTGGGCATAGGTCCAGCTCTGGTCACCTTCGAGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCCGCGCCTAGACCTTGGCCAGCGGCGCCTTGATCACGGTCAAACCGCGAAAgg                                                      >  4:310535/1‑149 (MQ=255)                                                                      ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGTCTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGcc                                                    <  2:256825/148‑1 (MQ=255)                                                                      ggTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCTCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGc                                                     <  7:233050/147‑1 (MQ=255)                                                                       gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                  <  8:58430/149‑1 (MQ=255)                                                                       gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCtt                                                  <  3:363295/149‑1 (MQ=255)                                                                             gTCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACtt                                            <  1:268438/149‑1 (MQ=255)                                                                              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                        <  2:221360/136‑1 (MQ=255)                                                                              tCCAGCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATa                                                        >  1:221360/1‑136 (MQ=255)                                                                                  gCTCTGGTCACCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAcc                                       >  5:244254/1‑149 (MQ=255)                                                                                        gTCACCTGCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGc                                   <  3:310535/147‑1 (MQ=255)                                                                                           aCCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                  >  5:63580/1‑113 (MQ=255)                                                                                           aCCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTca                                                                  <  6:63580/113‑1 (MQ=255)                                                                                           aCCTTCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt                              <  7:149675/149‑1 (MQ=255)                                                                                              ttCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt                              <  7:125853/146‑1 (MQ=255)                                                                                              ttCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCt                              >  8:125853/1‑146 (MQ=255)                                                                                              ttCGTGCTTGAGCAGC‑TTCTGCGACTTGCATTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCAc                           >  5:157814/1‑149 (MQ=255)                                                                                                                      acttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcc    <  6:157814/143‑1 (MQ=255)                                                                                                                       cttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcct   <  3:293654/144‑1 (MQ=255)                                                                                                                        ttgcaTTCGTGAATCCAGGCACAGGCCCCGGACACGTCCTCCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctc  >  2:110201/5‑149 (MQ=255)                                                                                                                                     |                                                                                                                                        CGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGAACC‑TCCGCCAACTGCAATTCGTGAACCAAGCAAAAGGCCCCGAAAAGGCCCTCC‑GCAGCCCCCCCAACCTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTC  >  NC_002947/4741124‑4741389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑