| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 96.2% | 167.5 / 0.3 | 52 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (23/27); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01 | |||||||||||
GCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACAT > NC_002947/1932493‑1932777 | gcggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑ccc < 8:23697/149‑2 (MQ=255) ttCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAggg > 5:108497/1‑149 (MQ=255) aaGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTg > 3:159303/1‑148 (MQ=255) gACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTg > 1:78905/1‑137 (MQ=255) gACATTCCACAGCGGCATTGCAGGTCTCACTAAAATAGCATTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTg < 2:78905/137‑1 (MQ=37) gaccggggCATTGCTGGTCTCACGAAATTAGCAGTAAAGGGTGGGGCCGCTTTGCGGCCCTTCGGGGG‑CAAGCCGGCTCCTACA‑ATGGATTTGCGTCCCCTGTAGGTGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTg < 2:248048/142‑1 (MQ=37) gACTGCGGCATTGCAGGTCTCACTAAAATACCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAgg < 1:326371/126‑1 (MQ=37) gCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGAc < 6:118396/149‑1 (MQ=255) gCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTa > 4:119967/1‑148 (MQ=255) gCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATCCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTa < 3:119967/148‑1 (MQ=255) cATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcg > 5:170914/1‑149 (MQ=255) cAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGAt > 1:97286/1‑149 (MQ=255) tctcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGc > 6:84256/1‑149 (MQ=255) tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGa > 5:327443/1‑149 (MQ=255) actaaaaTCGCAGTAACCGCGGGGGCCGTTTTGCGGCCGTGGGCGGG‑CAAGCCCGCTCATAAA‑ACGGATATGCGCCCCTTTTTGGAGGGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc < 4:159303/146‑1 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTACTACA‑AAGGATATGAGTGCCCGGTAGGAGCGGGCTTGG‑CC‑GCGAAGGGCTTGAAGGCAGCCCCAAATTTTCGACAACCCGGATACGCGAta > 8:175089/1‑148 (MQ=37) gctttAAACGGTGTGGCCGCTTTGCGGCCGTTGGCTGG‑CAAGCCGGCGACTACA‑AAGGTTATTCGTCCCCTGTAGTAGCGGGATT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGt < 5:132192/145‑1 (MQ=255) gaaGTATACGCTGTGGGCGCTTTGCGGCCCTTCTCGGG‑CAAGCCCGCTCATACA‑AATGATATGCGTCCCCTGTAGGAGCTGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGt < 5:138182/147‑1 (MQ=38) aGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGa > 3:332182/1‑149 (MQ=255) gTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGCTCAGCGATCCGGCAGGGTGAt > 7:330025/1‑149 (MQ=255) aaaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg < 6:327443/148‑1 (MQ=255) aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc < 5:84256/149‑1 (MQ=255) aCGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGCATATGCGGCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc < 3:170661/149‑1 (MQ=255) tGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCGACA‑AAGGATATGCGTCCCCGGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCCAGGCCGCCCCAAATTGTCGACTACGCGGATCAGCGAtg > 3:173367/1‑128 (MQ=37) tGGGGCCGCTTGGCGGCCCTGCGCGGC‑CACCCCCGCTCCTACA‑ACGCATTTGCGCCCCCGGTAGCCGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc < 4:173367/129‑1 (MQ=25) gCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCCCAACTACA‑AAGGATATTCGTCCCCGGTAGGAGCGGGCGT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTTTGGACAAAGCCGATCAGCGATCCGGGAGGGTGATGTTCAGCtg > 2:164353/1‑145 (MQ=25) cGCTTTGCGGCCCTTCGCGGGCCAACCCCCAACAAAAA‑AAGGATATGCGTCCCATTTAGAACCGGGATT‑GCCC‑CCCAAGGCCTGAAAGCCACCCCCAAAACGACGAAAACCCGGAACAGCGAACCGCAAGGGTGATTTTAAgaacgga > 2:347593/1‑142 (MQ=255) cGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCATACA‑ATGTTTATGCGTCCCCTTTCGGTGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATCGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt < 1:347593/138‑1 (MQ=37) cGGCCCTTGGCGGG‑AAAGCCGGATCATACA‑ATGTATTTGCGTCCCGTGTAGTTGGGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca < 2:97286/149‑1 (MQ=255) ggCCCTTCGCGGG‑CAAGCCCGCTCCTACAGAAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGa > 1:247105/1‑127 (MQ=255) ggCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGa < 2:247105/126‑1 (MQ=255) g‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt > 6:115619/1‑149 (MQ=255) g‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt > 5:3239/1‑149 (MQ=255) acgCCCGCTCCTCCA‑ATGGATTTGCGTCCCAGGTCGTAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 8:330025/147‑1 (MQ=38) aaGCCCGCTCCTACA‑AAGTATATGCGCCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc < 3:157939/149‑1 (MQ=255) cTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata < 4:332182/149‑1 (MQ=255) cTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGa < 6:108497/147‑1 (MQ=255) aCA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg > 6:62545/1‑149 (MQ=255) a‑aaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc > 4:237938/1‑115 (MQ=255) a‑aaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 3:237938/115‑1 (MQ=255) a‑aaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGca < 7:175089/149‑1 (MQ=255) aaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagc < 4:264838/118‑1 (MQ=255) aaGGATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagc > 3:264838/1‑118 (MQ=255) ggATATGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcat > 5:398976/1‑149 (MQ=255) tGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag < 4:278998/110‑1 (MQ=255) tGCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag > 3:278998/1‑110 (MQ=255) ggCGGCCCCTGTAGGAGCGGGCTG‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 7:156347/147‑1 (MQ=255) gCGTCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGt > 8:262082/1‑149 (MQ=255) tCCCCTGTAGGAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 5:62545/149‑1 (MQ=255) ggAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 7:244234/148‑1 (MQ=255) ggAGCGGGCTT‑G‑CCAGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCCGCAGGGTGATGTTCATCTCCAGAATCGAGCAGCTCCCCTCGTTTACCAGCTCGATATGCACATCATCGTTGCCGATGTTca > 6:410548/1‑149 (MQ=255) gAGCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTca > 4:411125/1‑148 (MQ=255) gCGGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACat < 6:3239/149‑1 (MQ=255) | GCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGG‑CAAGCCCGCTCCTACA‑AAGGATATGCGTCCCCTGTAGGAGCGGGCTT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACAT > NC_002947/1932493‑1932777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |