Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	307,866	+C	intergenic (+881/‑988)	hslO → / → PP_0254	molecular chaperone HslO/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	307,865	1	.	C	100.0%	255.3 / NA	74	intergenic (+880/‑989)	hslO/PP_0254	molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (35/39);  total (35/39)

CATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTAC  >  NC_002947/307722‑308007                                                                                                                                                 |                                                                                                                                                cATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgt                                                                                                                                             >  1:364215/1‑145 (MQ=255)   tGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTAc                                                                                                                                           <  1:269712/149‑1 (MQ=255)     ttCTCGGTGCAGAACTTCCTGCTTCAGGCTGCCGATGTACTGCTTATGCACTGCGCGTCCAACATCGGCGAAGAAGGAGATGTGACCTTGTTCTTAGGCCTGTCAGGCACTGGCAAGACTACACTTTCTGCTGACTAAAGCCGTTACCt                                                                                                                                         >  5:415110/1‑149 (MQ=255)          ggTGCAGAACTTCCTTCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGTCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCg                                                                                                                                    <  7:336519/149‑1 (MQ=255)             gCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGt                                                                                                                                  >  1:354686/1‑148 (MQ=255)              cAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTg‑a                                                                                                                               <  2:259199/149‑1 (MQ=255)               aGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTg‑ac                                                                                                                              <  3:246655/149‑1 (MQ=255)               aGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTg‑ac                                                                                                                              <  7:275765/149‑1 (MQ=255)                 aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTg‑acga                                                                                                                            >  5:437933/1‑149 (MQ=255)                  aCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTg‑acgac                                                                                                                           <  2:364215/149‑1 (MQ=255)                   cTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTg‑acgac                                                                                                                           >  6:114386/1‑148 (MQ=255)                        tgctgcCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCAc                                                                                                                     >  7:120119/1‑149 (MQ=255)                           tgccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGt                                                                                                                  >  2:420638/1‑149 (MQ=255)                             ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCTAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTg                                                                                                                <  7:238996/149‑1 (MQ=255)                                gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTgggg                                                                                                             <  2:132389/149‑1 (MQ=255)                                    ccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGaa                                                                                                         <  8:320863/149‑1 (MQ=255)                                       aTGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGc                                                                                                      >  2:240099/1‑149 (MQ=255)                                          tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTGCACGACGAGCACGGTTGGGGCGAAGGCGt                                                                                                    <  1:240099/149‑1 (MQ=255)                                            cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTAcc                                                                                                                                          >  2:252360/1‑109 (MQ=255)                                            cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTAcc                                                                                                                                          <  1:252360/109‑1 (MQ=255)                                             tGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTc                                                                                                >  4:306997/1‑149 (MQ=255)                                               ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCtt                                                                                              >  8:210641/1‑149 (MQ=255)                                                cTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTc                                                                                             <  3:306997/149‑1 (MQ=255)                                                   tGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                           <  6:437933/148‑1 (MQ=255)                                                                cAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgct                                                                             >  1:333231/1‑149 (MQ=255)                                                                 aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTg‑acgac                                                                                                                           >  5:251621/1‑102 (MQ=255)                                                                 aaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTg‑acgac                                                                                                                           <  6:251621/102‑1 (MQ=255)                                                                       tgCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCTGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                      <  8:146572/148‑1 (MQ=255)                                                                        gCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTCGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGc                                                                      >  6:119314/1‑148 (MQ=255)                                                                          gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa                                                                   <  1:108257/149‑1 (MQ=255)                                                                            agaagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGt                                                                 <  8:120119/149‑1 (MQ=255)                                                                                ggCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCAt                                                             >  3:269798/1‑149 (MQ=255)                                                                                        gACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcgg                                                                               >  5:341375/1‑123 (MQ=255)                                                                                        gACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcgg                                                                               <  6:341375/123‑1 (MQ=255)                                                                                        gACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCATTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGt                                                     >  5:142039/1‑149 (MQ=255)                                                                                           cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                           <  4:194002/108‑1 (MQ=255)                                                                                           cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                           >  3:194002/1‑108 (MQ=255)                                                                                           cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                   >  2:209559/1‑148 (MQ=255)                                                                                            ttGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                         <  6:276224/141‑1 (MQ=255)                                                                                            ttGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                         >  5:276224/1‑141 (MQ=255)                                                                                              gTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCgag                                                <  2:102785/148‑1 (MQ=255)                                                                                              gTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCga                                                 <  2:333231/147‑1 (MQ=255)                                                                                               ttcttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaa                                              <  5:114386/149‑1 (MQ=255)                                                                                                 cttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcg                                                                                >  3:33852/1‑113 (MQ=255)                                                                                                 cttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcg                                                                                <  4:33852/113‑1 (MQ=255)                                                                                                 cttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaag                                             >  5:327758/1‑148 (MQ=255)                                                                                                  ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                           <  4:269798/149‑1 (MQ=255)                                                                                                  ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAgaagaa                                           >  8:128523/1‑149 (MQ=255)                                                                                                    cGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaaga                                            <  7:210641/146‑1 (MQ=255)                                                                                                    cGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaaga                                            <  1:420638/146‑1 (MQ=255)                                                                                                        cTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGcc                                     >  4:81302/1‑149 (MQ=255)                                                                                                           tCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGt                                  >  8:91097/1‑149 (MQ=255)                                                                                                            ccGGCACTGGCAAGACTAACCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCAAGGTTGGGGCCAAGGCGTTGTCTTCAACATGGAAGTAGGCTGATACGCCAAGTGCATCGACCAGTACGAGAAGAACGAGCCgtg                                  >  4:225414/1‑146 (MQ=255)                                                                                                             cGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTc                                 >  8:20221/1‑148 (MQ=255)                                                                                                                cACTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCt                             <  6:327758/149‑1 (MQ=255)                                                                                                                 aCTGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTg                            <  2:354686/149‑1 (MQ=255)                                                                                                                   tGGCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTgg                           >  5:287074/1‑148 (MQ=255)                                                                                                                     gCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                           <  5:198344/82‑1 (MQ=255)                                                                                                                     gCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCaa                                                                                           >  6:198344/1‑82 (MQ=255)                                                                                                                     gCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGa                          >  8:293800/1‑147 (MQ=255)                                                                                                                     gCAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCATTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGaaa                        <  6:142039/149‑1 (MQ=255)                                                                                                                      cAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                          <  1:208647/130‑1 (MQ=255)                                                                                                                      cAAGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                          >  2:208647/1‑130 (MQ=255)                                                                                                                       aaGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTGAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaaga                                            >  1:135877/1‑127 (MQ=255)                                                                                                                       aaGACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGagaaga                                            <  2:135877/127‑1 (MQ=255)                                                                                                                         gACTACCCTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCa                    >  6:69008/1‑149 (MQ=255)                                                                                                                              cccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCa                 <  3:81302/147‑1 (MQ=255)                                                                                                                               ccTTTCGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGc              >  8:389656/1‑149 (MQ=255)                                                                                                                                    cGGCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACgg          <  7:91097/148‑1 (MQ=255)                                                                                                                                      gCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTTCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                   >  5:178822/1‑137 (MQ=255)                                                                                                                                      gCTGACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTTCGAGAAGAACGAGCCGGTCATCTGGAAAGCCAt                   <  6:178822/137‑1 (MQ=255)                                                                                                                                         gACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgctgct                                                                          >  3:76916/1‑79 (MQ=255)                                                                                                                                         gACGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgctgct                                                                          <  4:76916/79‑1 (MQ=255)                                                                                                                                          aCGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTa   <  7:389656/149‑1 (MQ=255)                                                                                                                                           cGAAAGCCGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTAc  <  5:69008/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                CATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGTTACCTGATCGGTG‑ACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTAC  >  NC_002947/307722‑308007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑