Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	336,125	+T	coding (210/237 nt)	PP_0278 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	336,124	1	.	T	98.5%	228.4 / ‑3.6	68	coding (209/237 nt)	PP_0278	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (30/37);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

AACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335979‑336263                                                                                                                                                    |                                                                                                                                            aaCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTACGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑tt                                                                                                                                           <  8:10258/148‑2 (MQ=255)  aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑ttac                                                                                                                                         <  5:389043/149‑4 (MQ=255)  aCCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑ttac                                                                                                                                         <  2:131776/149‑4 (MQ=255)    ccATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGAATGTCTCGATCCTGATGGGCTAAATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGAAGCC‑GA‑ttacct                                                                                                                                       >  7:158158/1‑144 (MQ=255)             aaTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGAc                                                                                                                                 <  4:406357/147‑1 (MQ=255)                   cTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATgg                                                                                                                             >  1:298766/1‑145 (MQ=255)                   cTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATgg                                                                                                                             <  2:298766/145‑1 (MQ=255)                      cgcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGgc                                                                                                                      <  7:330425/149‑1 (MQ=255)                        cgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGTTGGGCTACATCAAGCACCTGGTGCGCTAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGgcgc                                                                                                                    <  6:109265/149‑1 (MQ=255)                         gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCt                                                                                                                   >  3:273148/1‑149 (MQ=255)                         gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑ttacct                                                                                                                                       >  4:229611/1‑123 (MQ=255)                         gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑ttacct                                                                                                                                       <  3:229611/128‑6 (MQ=255)                                ccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAAc                                                                                                            <  1:33232/149‑1 (MQ=255)                                    ggtcacagTCGACGCGGCCTACTAGGATGTATCGATCCTGATGTGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGata                                                                                                        <  8:158158/142‑1 (MQ=255)                                    cGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGAGAGGGCGATATGGATGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGata                                                                                                        <  7:228363/149‑1 (MQ=255)                                           gTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGAt                                                                                                 <  7:88404/149‑1 (MQ=255)                                               aCGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTTTGCGAAATGGCCAAGGCGCTGATGAACGATATCTAGATCGc                                                                                              >  3:29583/1‑148 (MQ=255)                                               aCGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGcc                                                                                             <  3:308632/149‑1 (MQ=255)                                                 gctgCCTACGCTCATGTCGCGATCCTGATGGGCGACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCaa                                                                                           <  4:29583/146‑1 (MQ=255)                                                      cTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt                                                                                       >  2:142545/1‑148 (MQ=255)                                                        aagAGCATGTCTCGATACTGATGGGCTACATCAAGCACCTGGTTCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGTGGGCAGCC‑GATTACATGTGCGACATTGCAAAGGCGCTGATTAACTATATCGAGATCGCCAAGGGTAAg                                                                                    >  3:6749/3‑149 (MQ=255)                                                         cGAGTATGTCTCCATCATGATGGGCTACATCAAGCCCCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑TATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTaa                                                                                     <  8:290556/147‑1 (MQ=255)                                                         cGAGCATGTCTCGATCCTGATGGTCTACATCAAGCACCTGGTGCGCGATGGAGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAg                                                                                    <  1:51996/148‑1 (MQ=255)                                                           aGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgcg                                                                                 >  1:25120/1‑149 (MQ=255)                                                            gCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgcg                                                                                 >  1:40125/1‑148 (MQ=255)                                                                 tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAg                                                                           >  2:98198/1‑149 (MQ=255)                                                                 tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGAGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCaa                                                                                           <  5:45945/133‑1 (MQ=255)                                                                 tctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGAGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCaa                                                                                           >  6:45945/1‑133 (MQ=255)                                                                  ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgg                                                                          >  6:323731/1‑149 (MQ=255)                                                                  ctcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgg                                                                          >  7:369743/1‑149 (MQ=255)                                                                   tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgg                                                                          >  3:400596/1‑148 (MQ=255)                                                                   tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgg                                                                          >  6:208882/1‑148 (MQ=255)                                                                   tcGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAgg                                                                          >  3:128070/1‑148 (MQ=255)                                                                     gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCa                                                                                                                          <  6:69746/98‑1 (MQ=255)                                                                     gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCa                                                                                                                          >  5:69746/1‑98 (MQ=255)                                                                     gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAt                                                                       <  5:208882/149‑1 (MQ=255)                                                                      aTCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGc                                                                                                                                    >  1:47475/1‑87 (MQ=255)                                                                      aTCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGc                                                                                                                                    <  2:47475/87‑1 (MQ=255)                                                                         cTGATGGGCAACATCAAGCACCTGTTGCTCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTTGGACATGGCCAAGGCGCTGATGAACTATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGa                                                                    <  7:222269/148‑1 (MQ=255)                                                                           gATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcgc                                                                 >  1:419564/1‑149 (MQ=255)                                                                              gggCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGt                                                              >  5:438037/1‑149 (MQ=255)                                                                                gCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCa                                                            >  4:383094/1‑149 (MQ=255)                                                                                 cTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAt                                                           >  1:227701/1‑149 (MQ=255)                                                                                 cTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAt                                                           <  5:323731/149‑1 (MQ=255)                                                                                     aTCAAGCACCCGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       <  5:67765/149‑1 (MQ=255)                                                                                      tCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACTATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                      <  2:419564/149‑1 (MQ=255)                                                                                      tCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgtg                                                      >  7:79122/1‑149 (MQ=255)                                                                                          gCACCTTGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGTGTGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAg                                                  <  6:438037/149‑1 (MQ=255)                                                                                             ccTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCgg                                               >  1:284908/1‑149 (MQ=255)                                                                                                 gTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGcc                                             <  3:383094/147‑1 (MQ=255)                                                                                                   gcgcGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAg                                                  <  8:1948/140‑1 (MQ=255)                                                                                                   gcgcGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAg                                                  >  7:1948/1‑140 (MQ=255)                                                                                                     gcgAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGt                                       <  2:40125/149‑1 (MQ=255)                                                                                                          ggCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGa                                  <  8:369743/149‑1 (MQ=255)                                                                                                           gCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgc                                                                                  <  8:333794/100‑1 (MQ=255)                                                                                                           gCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGgc                                                                                  >  7:333794/1‑100 (MQ=255)                                                                                                                  ggagtaCGACCATAATTTCGTGGGGGCAGCC‑GATTACCTGTGCGACATTGCGAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                         <  4:7931/134‑1 (MQ=255)                                                                                                                  ggaggaCGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGTCATTGACGCGGTCATTTGTGGGAGCGGCCTTGc                                         >  3:7931/1‑134 (MQ=255)                                                                                                                  ggaggaCGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTg                          >  5:127343/1‑149 (MQ=255)                                                                                                                   gaggaCGACCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTg                          >  3:119769/1‑148 (MQ=255)                                                                                                                       acgacCATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAag                     <  6:136682/149‑1 (MQ=255)                                                                                                                       aagacCATAAATTCCTGGGTGCAGCC‑TATTACCTGTGCGACATGGCCAAGGCGATGATGAACCATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTAATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCaaa                      <  8:349903/146‑1 (MQ=255)                                                                                                                         gacCATAAATTACTGTGTTCAGAC‑GATTACCTGTTCTACATGGCCAAGGCTCTGATGAACTATATCTAGATCGCCAAGTGTAAGGCGCATTAGTCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCTTCGCGAATGGTCTGCAAagca                   <  4:6749/149‑1 (MQ=255)                                                                                                                           ccATAAATTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCAAagcggc                 >  6:338297/1‑149 (MQ=38)                                                                                                                                 aTTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGcc                                                                                                                           >  5:369066/1‑37 (MQ=255)                                                                                                                                 aTTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGcc                                                                                                                           <  6:369066/37‑1 (MQ=255)                                                                                                                                 aTTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCTTTGCAGcccattc           <  1:142545/149‑5 (MQ=39)                                                                                                                                 aTTCCTGGGGGCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGc           >  2:187896/1‑149 (MQ=255)                                                                                                                                    cgtGGGGGCAGCCTTT‑TACCTGTGCGACTTGGCCAAGGCGCTTATTAACTATATCGATATCGCCAAGGTTAAGGCGCATTAGGCATTGACGCGGTCTTTTGTGGGAGCGGCCTTGCGTCGCTAATGGGCTGAAAAGCAGCCCCGGCGa         <  2:227701/146‑1 (MQ=255)                                                                                                                                        ggggCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTc     <  4:273148/148‑1 (MQ=255)                                                                                                                                        ggggCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCa    <  2:284908/149‑1 (MQ=255)                                                                                                                                          ggCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga  <  1:98198/149‑1 (MQ=255)                                                                                                                                          ggCAGCC‑GATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga  <  4:128070/149‑1 (MQ=255)                                                                                                                                          ggCAGCC‑GATTACCTGTGCGACAAGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga  <  4:317904/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                            AACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCC‑GA‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335979‑336263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑