Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,419,549 +C intergenic (‑38/+98) PP_1242 ← / ← PP_1244 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,419,5481.C97.4% 125.6 / ‑3.5 39intergenic (‑37/+99)PP_1242/PP_1244hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (19/19);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑C‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATG  >  NC_002947/1419401‑1419679
                                                                                                                                                     |                                                                                                                                    
cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                     <  7:111847/149‑1 (MQ=255)
cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                     <  3:391261/149‑1 (MQ=255)
cTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGggctgg‑c                                                                                                                                     <  3:30157/149‑1 (MQ=255)
       gggATGCCGGGTTCGATGCGGAACAGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTcg                                                                                                                              >  6:150043/1‑149 (MQ=255)
        ggATGCCGGGTTCGATGCGGAACAGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTcgc                                                                                                                             >  8:35659/1‑149 (MQ=255)
         gATGCCGGGTTCGATGCGGAACAGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTcgcg                                                                                                                            <  6:388551/149‑1 (MQ=255)
          aTGCCGGGTTCGATGCGGAACAGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCgg                                                                                                                           <  5:150043/149‑1 (MQ=255)
            gCCGGGTTCGATGCGGAACAGCGGGTGAGGTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCggg                                                                                                                          >  1:31683/1‑149 (MQ=255)
              cGGGGTGGATGCGGAACACCGGGGTT‑GGGTTGTTTTCGCCTTGGTAGCAGCCCGTCGTGCCGACTGTGGTGGGGTCTGGTGTGTTGTCGGTGGACATTGTTTTGTTTACCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCgg                                                                                                                           <  7:325552/145‑1 (MQ=255)
                         cGGAACAGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGGTTTGTGTCCCTGGATTCTGGGTTGCCCGGGCTGGCC‑TCTTCGCGGGCGTGCCCGcgaag                                                                                                             <  3:190329/148‑5 (MQ=255)
                               aGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTa                                                                                                      <  4:312553/149‑1 (MQ=255)
                                   ggTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACtgt                                                                                                  <  7:35659/149‑1 (MQ=255)
                                          tgttgtTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTcgcg                                                                                                                            <  2:86580/117‑1 (MQ=255)
                                          tgttgtTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTcgcg                                                                                                                            >  1:86580/1‑117 (MQ=255)
                                           gttgttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGc                                                                                           >  6:82147/1‑149 (MQ=255)
                                            ttgttTTCGCCTTGGTTGAAGCAGGTCTTGCAGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGACCGCTCCCACAGGTACTGTGCAGTGCg                                                                                          >  5:421063/1‑149 (MQ=39)
                                            ttgttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCg                                                                                          >  3:392657/1‑149 (MQ=255)
                                              gttTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGt                                                                                         <  4:392657/148‑1 (MQ=255)
                                                         ggTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCATGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGt                                                                                             >  7:174230/1‑133 (MQ=255)
                                                            aGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                    <  5:228611/123‑1 (MQ=255)
                                                            aGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACt                                                                                                    >  6:228611/1‑123 (MQ=255)
                                                            aGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGAc                                                                          >  2:299632/1‑149 (MQ=255)
                                                                     tCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTgg                                                                   >  2:14359/1‑147 (MQ=255)
                                                                          ccGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCgg                                                             <  2:31683/148‑1 (MQ=255)
                                                                          ccGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCgg                                                             <  5:82147/148‑1 (MQ=255)
                                                                           cGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGCTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGc                                                           <  1:299632/149‑1 (MQ=255)
                                                                                 tggtggGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGa                                                                                       >  6:286452/1‑115 (MQ=255)
                                                                                 tggtggGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGa                                                                                       <  5:286452/115‑1 (MQ=255)
                                                                                 gcgttggTGCTGTTGTGTTGTCGGTGGTCATTTTTTTGTTTCCCTTGAATCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGATCCCACAGTTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGtcc                                                     <  6:421063/144‑1 (MQ=37)
                                                                                     gggTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCACGCCcgctc                                                 <  5:103427/149‑3 (MQ=255)
                                                                                           tgttgtgttgTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCAAAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGc                                           >  5:90885/1‑149 (MQ=255)
                                                                                                              aTTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAgg                        >  6:91744/1‑149 (MQ=255)
                                                                                                                tgttttgtttCTGTGTTTTGTGGGTTGCGTGGGTTGG‑CTTCTTGGCGGGCATGCCCGTTCCCAGTGTTACTTTGCAGTGGGTGAGGGTTGTGGTGACGGTGTGGGAGGGGGGGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGgt                       <  2:90477/148‑1 (MQ=255)
                                                                                                                  ttttgtttCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgtg                                                                               >  1:403695/1‑90 (MQ=255)
                                                                                                                  ttttgtttCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCtgtg                                                                               <  2:403695/90‑1 (MQ=255)
                                                                                                                  ttttgtttCCCTGGATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCCCTCCAACAGGTACAGTGGATTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGCGCGTGCCACCGAAGAGGCCTGACCTGCTAATGCAAGgt                       >  1:90477/1‑146 (MQ=255)
                                                                                                                               gATTCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTgg         <  5:91744/147‑1 (MQ=255)
                                                                                                                                  tCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTc                                                                                                               <  6:11432/42‑1 (MQ=17)
                                                                                                                                  tCTGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTc                                                                                                               >  5:11432/1‑42 (MQ=17)
                                                                                                                                    tGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGc                                                           <  6:257686/92‑1 (MQ=255)
                                                                                                                                    tGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGc                                                           >  5:257686/1‑92 (MQ=255)
                                                                                                                                    tGGGTTGCCTGGGCTGGCC‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCAGTGGTGACCCTGTGGGAGCGGGCCTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATg  >  6:437579/1‑149 (MQ=255)
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CTGGCAGGGGATGCCGGGTTCGATGCGGAACAGCGGGTGA‑GTGTTGTTTTCGCCTTGGTAGAAGCAGGTCTTGCCGACTGTGGTGGGTTCTGTTGTGTTGTCGGTGGGCATTGTTTTGTTTCCCTGGATTCTGGGTTGCCTGGGCTGG‑C‑TCTTCGCGGGCACGCCCGCTCCCACAGGTACTGTGCAGTGCGTGACGGCTGTGGTGACCCTGTGGGAGCGGGCGTGCCCGCGAAGAGGCCTGACCTGCTAATGCAAGGTGTGAGGCTCTGTGGGTAAATG  >  NC_002947/1419401‑1419679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑