Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 2,921,460 G→A G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,921,4600GA96.4% 175.3 / NA 58G3494R (GGG→AGG) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (26/27);  minor base T (0/2);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATT  >  NC_002947/2921317‑2921604
                                                                                                                                               |                                                                                                                                                
cGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCaggggg                                                                                                                                             <  7:229814/149‑1 (MQ=255)
 gTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagggggg                                                                                                                                            >  6:67400/1‑149 (MQ=255)
   tGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGCCGCTGGAGATGACAGCCTGGATGGCGGAAAAGGCAATGACACCCTGCAGGCCGCCGATGGTAACGACAAATTGTTCGGCGACGTGGCTACCACAACACTGAACaggggggac                                                                                                                                          >  5:259965/1‑147 (MQ=255)
    gCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGcc                                                                                                                                          <  5:64355/148‑1 (MQ=255)
     ttGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGcc                                                                                                                                          <  1:242266/146‑1 (MQ=255)
     cTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCg                                                                                                                                         <  7:35745/148‑1 (MQ=255)
      tGGGGGAGGCCGGTAACGACAACATGAACGGCGGCGCTGGCGATGAAAGCCTGGATGGCGGCAACGGCAATGAAACCCTGAAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCgg                                                                                                                                        >  2:196705/1‑148 (MQ=255)
       gggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGcc                                                                                                                                          >  2:242266/1‑145 (MQ=255)
        ggggAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCaa                                                                                                                                     <  7:110319/149‑1 (MQ=255)
        ccggAGGCCGGTACCGTCACCCGGTACGGGGGCGCTGGCGATGACAGCCTTGATGGAGGCAACGGCAATGACTCCCTGCAGGGCGGCGATGGTAACGACACTTTGTTCGGCGACGTGGGTTCCGACACCCTGATCAGGGGGGCCGGCaa                                                                                                                                     <  8:258671/147‑1 (MQ=255)
            aGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGa                                                                                                                                  >  1:180811/1‑148 (MQ=255)
            aGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGAc                                                                                                                                 <  2:180811/149‑1 (MQ=255)
                 ggTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCt                                                                                                                            <  7:39828/149‑1 (MQ=255)
                  gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCgg                                                                                                                                        >  5:139337/1‑136 (MQ=255)
                  gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCgg                                                                                                                                        <  6:139337/136‑1 (MQ=255)
                   tAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATGAGGGGGGCCGGCAACGACTTCCTCa                                                                                                                          <  8:115241/149‑1 (MQ=255)
                     aCGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAAc                                                                                                                                    <  8:53295/137‑1 (MQ=255)
                     aCGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAAc                                                                                                                                    >  7:53295/1‑137 (MQ=255)
                     aCGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAAc                                                                                                                        <  1:15412/149‑1 (MQ=255)
                            ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                              >  3:282659/1‑136 (MQ=255)
                            ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                              <  4:282659/136‑1 (MQ=255)
                            ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                                 >  5:406741/1‑149 (MQ=255)
                            ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                                 <  3:99095/149‑1 (MQ=255)
                             cTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                                 >  1:396114/1‑148 (MQ=255)
                                       tggcTGGCTATGACAGCCTGGATGGCGGCAACGGAAATGACACCCTGCAGGGCGGCTATGGTAACGACACATTGTTCGGCGACGGGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGAt                                                                                                      <  6:288007/147‑1 (MQ=255)
                                             gCGATGACAGCCTGGATGTCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggt                                                                                                 <  5:67400/148‑1 (MQ=255)
                                             gCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtg                                                                                                >  1:284723/1‑149 (MQ=255)
                                                    cAGCCTGGATTGCTTCAACGGCAATGACACCCTGGTGTGCGTCGATTGTAAGTACACTTTGTTCGTCGACGTGGGTTCCGACAGCCTGATGTGGGTGGCCGGTAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggtg                                                                                         <  6:259965/149‑1 (MQ=255)
                                                        cTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGCTGCGACAGGTAACGATACGGTGGTGGGTGGGGc                                                                                     >  2:53054/1‑149 (MQ=255)
                                                                 ggcaacggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGa                                                                            <  2:284723/149‑1 (MQ=255)
                                                                        gcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCAt                                                                      >  8:232211/1‑148 (MQ=255)
                                                                               cacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCgg                                                                                  >  6:114100/1‑129 (MQ=255)
                                                                               cacCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCgg                                                                                  <  5:114100/129‑1 (MQ=255)
                                                                                acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAc                                                             >  6:208588/1‑149 (MQ=255)
                                                                                   cTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGa                                                          >  3:311161/1‑149 (MQ=255)
                                                                                         ggcggcGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGaaa                                                    <  5:208588/149‑1 (MQ=255)
                                                                                             gcgATGGTAACGACACATTGTTCTGCGACGTGGTTACCGACACCCTGATCATGGGGGCCGGCAACGACTTCCTCAACGGTGCGCCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                                <  1:196705/149‑1 (MQ=255)
                                                                                              cgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAtg                                               >  8:59344/1‑149 (MQ=255)
                                                                                                   gTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATAATCGCCACCGATGGAAACGATGTGtt                                           <  2:396114/148‑1 (MQ=255)
                                                                                                      aggtcacaTTGTTCGGCTACGTTGGTACCGAACCCCTTATCTGGGGGCCCGGCATCGACTTCCTCTACGGTGGGGGAGGTAACGATACGGTGGTGGGTGTGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCa                                        <  4:156127/144‑1 (MQ=255)
                                                                                                        gACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATg                                                        >  8:173043/1‑129 (MQ=255)
                                                                                                        gACACATTGTTCGGCGACGTGGGTACCGACACCCTGATC‑AGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATg                                                        <  7:173043/129‑1 (MQ=255)
                                                                                                           acaTTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGc                                  <  4:311161/149‑1 (MQ=255)
                                                                                                               tGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGCTACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTg                                >  5:140269/1‑147 (MQ=255)
                                                                                                                gTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAg                             >  1:406392/1‑149 (MQ=255)
                                                                                                                 ttCGGCGACGTGGGTACCGACACCCTGATCAGGGGGTCCGTCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGTAAACGATGTGTTCCAGTTCGATGCAgg                            >  1:273938/1‑149 (MQ=255)
                                                                                                                      cGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTg                       <  7:232211/149‑1 (MQ=255)
                                                                                                                       gACGTGCCTTCCGACACCCTGCGCCGGGGCGCCGGCAACGACTTCCTCCACGGGGCGGCAGGTAACGATACGGTGGTGGGGGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTgg                      <  1:385826/149‑1 (MQ=255)
                                                                                                                            gggTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAAc                  >  2:176343/1‑148 (MQ=255)
                                                                                                                               tACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGGCGGTAACGACACCATGATc                                                                  >  1:134646/1‑97 (MQ=255)
                                                                                                                               tACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATc                                                                  <  2:134646/97‑1 (MQ=255)
                                                                                                                               caccctcacCCTGATCAGGGGGGCCGGCATCGAATTCGTCAATGGTGCCTCAGGTAAAGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCATGCTTTGGCAACGAcc              <  6:110853/143‑1 (MQ=255)
                                                                                                                                aCCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCt             >  1:342104/1‑149 (MQ=255)
                                                                                                                                   gACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGat          >  4:402253/1‑149 (MQ=255)
                                                                                                                                    acacCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGat          >  6:386884/1‑148 (MQ=255)
                                                                                                                                      acCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGacac                                                                         >  1:89784/1‑83 (MQ=255)
                                                                                                                                      acCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGacac                                                                         <  2:89784/83‑1 (MQ=255)
                                                                                                                                       cccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat       >  3:260080/1‑148 (MQ=255)
                                                                                                                                        ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTaa                                                                               >  2:80983/1‑75 (MQ=255)
                                                                                                                                        ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTaa                                                                               <  1:80983/75‑1 (MQ=255)
                                                                                                                                            aTCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAAtt  <  3:402253/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAATT  >  NC_002947/2921317‑2921604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑