| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 82.6% | 39.9 / ‑2.4 | 25 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); major base C (7/12); minor base A (4/0); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 96.0% | 54.0 / ‑3.6 | 25 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (11/13); minor base C (0/1); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
GACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGT‑T‑TCGGGCA‑C‑G‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGA > NC_002947/1499355‑1499642 || gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGCAACAGGCGCCGCCGTGCATGGCACC‑GGGGTTGCCGGGGt‑t‑taacgg > 1:146222/1‑144 (MQ=38) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑c < 1:7079/149‑1 (MQ=37) gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑c‑g‑‑cc > 2:97398/1‑149 (MQ=37) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑c‑g‑‑cccg > 7:283673/1‑147 (MQ=25) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAAAAGGCGCCCCGGTGCATGGCACC‑CGCGTTGCCCGTGT‑TAGCGGGCA‑A‑GC‑CCGCTCCCACAGACAGAtt > 8:245191/1‑149 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCg < 8:283673/149‑1 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCCTGCCCCCGAAACACGCGCCGCGGTGCAAGGCACC‑CGCGTTGACCGTGT‑TAGCGGGCACC‑C‑‑CCCCACCCACAGACAGAATGACATTAAGCg > 8:380041/1‑149 (MQ=255) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCCCAGCCTGCTTGCCATTAAGCGGGCCTa > 5:317267/1‑149 (MQ=21) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc < 7:380041/149‑1 (MQ=21) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCAtt < 1:356361/124‑1 (MQ=18) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCCGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCCCTCCCACAGCCGGAATGCCATTAAGCGGGCCTATCGCGACAGTGACg > 7:127304/1‑149 (MQ=18) cagctgGACTGAGGCTGCCAAAGGGTGGGAGC‑GGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc < 6:317267/149‑1 (MQ=21) gcagGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGGGTTGCCGGTGT‑TAGCGGGCC‑C‑GC‑CCGGGCCCACAGACTGCTTGCCATTAAGCGGGGCTATCGCTACAGTGACGGccc > 6:204379/1‑149 (MQ=21) cagGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCg > 5:133090/1‑149 (MQ=21) agGACTGAGGCTGCCAAACGGTGGGAGCGGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATTGCACC‑GGCGTTGCCGGTGT‑TAGCGGGCC‑C‑GC‑CCGCTCCCACAGACTGATTTGCATTAATCGGGCCTATCGCTACACTGAAGGcccc > 1:240375/1‑148 (MQ=255) ggACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtt < 3:310397/149‑1 (MQ=21) ggACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtt < 1:97398/149‑1 (MQ=21) ccAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt < 5:437357/148‑1 (MQ=21) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg > 5:353754/1‑130 (MQ=21) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg < 6:353754/130‑1 (MQ=21) tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGTCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc < 2:280110/149‑1 (MQ=25) ccccGCTAAACCGCCCCCGCGGTG‑AAGGCCACGGGCTTTGCGGTTGT‑C‑CGCGGCA‑A‑GGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 3:221257/148‑1 (MQ=255) cccGCGAAACTGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTGC‑GCGGGCA‑CGG‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGAGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 2:146222/148‑1 (MQ=37) aaaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACAGATTGCCATTAAGCGGGCCTATCGCTa < 4:22372/94‑1 (MQ=17) aaaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACAGATTGCCATTAAGCGGGCCTATCGCTa > 3:22372/1‑94 (MQ=17) cgcgGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCa < 2:240375/149‑1 (MQ=37) ttcgcGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGa < 8:127304/145‑1 (MQ=255) gcGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt > 7:188077/2‑122 (MQ=255) gcGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCt < 8:188077/121‑1 (MQ=255) || GACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGT‑T‑TCGGGCA‑C‑G‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGA > NC_002947/1499355‑1499642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |