Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,819	2 bp→CT	intergenic (+56/+696)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,819	0	T	C	100.0%	145.8 / NA	48	intergenic (+56/+697)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/26);  total (22/26)
*	NC_002947	4,740,820	0	G	T	97.9%	143.3 / NA	48	intergenic (+57/+696)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (22/25);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACC  >  NC_002947/4740679‑4740953                                                                                                                                             ||                                                                                                                                       cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                >  5:263177/1‑149 (MQ=255) cAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCACAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTCTTTATTTGTCCTGTGCCGGc                                                                                                                                <  4:111140/149‑1 (MQ=255)  aGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGc                                                                                                                                >  4:411417/1‑148 (MQ=255)             gATCGGCCGTCCTCGCCAGCTGTACTCCGGCGAGCAGAAGCGCGACATCGTTGCGCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGcc                                                                                                                   <  3:38660/149‑1 (MQ=255)              aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGACAGACCGCAAGTAAGCTGCAGTAGCCGACCGCAAGAAGGCTGCCCTCGGGCAGCC‑GTTTTTATTTGTCCTGGGCCGGCCTC‑TTCGCGGCCt                                                                                                                  >  3:342758/1‑149 (MQ=255)              aTCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCt                                                                                                                  >  6:430180/1‑149 (MQ=255)               tCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCtt                                                                                                                 <  5:124189/149‑1 (MQ=255)                 ggCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCAC‑TTCGCGGCCtgtc                                                                                                               >  3:130348/1‑146 (MQ=255)                          cgcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAaga                                                                                                      <  2:279996/149‑1 (MQ=255)                           gcCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAagag                                                                                                     >  4:246569/1‑149 (MQ=255)                             cAGCTGTACACCGGCGAGCAGTAGAGCGACATCGTTGCCCTGAAAGACCGAAAGTAAGCTGCAGTAGCCGAACGCAATAAGTCTGCCCTCGGGCAGCCTTTTTTT‑TTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCGCGCGAAGAGGc                                                                                                   <  4:342758/149‑1 (MQ=255)                                      acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGa                                                                                          <  7:125568/149‑1 (MQ=255)                                                 gacgcgcGACCTCGTGGCCCTGAAACACCTCACTTACGCGGCATCAGCCTACCCCAACAGGGCTCCCCTC‑GGCATGCTTTTTTTATTTGCCCTGGGCCGGCCTC‑TTCGCGGCCTTGCCCGCTATGTGGCCGGCACAGACACCACAAAAc                                                                               <  4:130348/146‑1 (MQ=255)                                                        gACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACATACGCGCCATTCCCCGCAAAGAGCCCGG‑ACACGCCCCACAAAAccaaaatt                                                                        >  2:361180/1‑143 (MQ=255)                                                             cGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGGCAAGGCCGCGAaga                                                                                                      >  7:396546/1‑114 (MQ=37)                                                             cGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGGCAAGGCCGCGAaga                                                                                                      <  8:396546/114‑1 (MQ=37)                                                             cGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAagag                                                                                                     <  4:412773/115‑1 (MQ=255)                                                             cGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAagag                                                                                                     >  3:412773/1‑115 (MQ=255)                                                             cGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTAGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑ATCGCGGCCTTGCCCGCGAAGACGCCGGCACAGACACCACAAAACCGCAGGTTTtc                                                                    >  5:402927/1‑148 (MQ=255)                                                              gTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTACCGGCCTTTCCCGCGCAGAGGCCGGCACCGACCCCCCAAAACCGCAGGTTTtatc                                                                  >  6:364524/1‑149 (MQ=255)                                                               ttGCCCTCAAAGACCGCAAGTAAGCTGCAGTCGCCCAACCCAACAAGGCTGCCCTCGGCCAGCC‑TTTTTTATTTGTCCTGGGCCGGCCTC‑TTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTcc                                                                 <  4:374778/149‑1 (MQ=255)                                                                    cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTACTccgcc                                                              >  7:373840/1‑147 (MQ=255)                                                                    cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTACTccgcc                                                              <  8:373840/147‑1 (MQ=255)                                                                    cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCAC‑TTAGCGGCCTTGCCCGCGCAGAGGCCGGCACAGCCACCACAAAACCACACTTTTTATacgcccg                                                            >  1:180492/1‑149 (MQ=255)                                                                     tGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGGCAAGGCCGCGAagag                                                                                                     <  1:332831/107‑1 (MQ=25)                                                                              gCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACCCCACAAAACCTCAGTTTTTCTACGCCCGCGAACGCAGc                                                  >  2:297777/1‑149 (MQ=255)                                                                               cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAaga                                                                                                      >  1:383707/1‑96 (MQ=38)                                                                               cAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAaga                                                                                                      <  2:383707/96‑1 (MQ=38)                                                                                gaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAagag                                                                                                     <  8:14847/95‑1 (MQ=38)                                                                                gaGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAagag                                                                                                     >  7:14847/2‑96 (MQ=38)                                                                                     aGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTc                                            <  6:337899/148‑1 (MQ=255)                                                                                       cTGCCGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGa                                                                                          <  5:433174/100‑1 (MQ=37)                                                                                       cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGa                                                                                          >  6:433174/1‑100 (MQ=38)                                                                                        tGCAGTAGCCGAACGCAAGAAGGCTGCCCTCTGGAAGCC‑TTTTTTATTTGTCCTGTTCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCATTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAggg                                        <  5:430180/149‑1 (MQ=255)                                                                                               gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGa                                  <  3:246569/148‑1 (MQ=255)                                                                                                        acgaagGCTCCCCTCGGGCAGCCTTTTTTT‑TTTGTCCTGTGCCGCCCTC‑TTCGCGGCCTTGCCCACGAAGAGGCCGGCACAGACACCACAAAACCTCAg                                                                          <  2:183369/97‑1 (MQ=37)                                                                                                        aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCGTGGCCCCGAAGAGGCCGGGACAGACACCACAAAACCTCAg                                                                          >  1:183369/1‑99 (MQ=25)                                                                                                               cTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACg                 <  3:411417/149‑1 (MQ=255)                                                                                                                     tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGcc                                                 >  6:332313/1‑111 (MQ=38)                                                                                                                     tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGcc                                                 <  5:332313/111‑1 (MQ=38)                                                                                                                     tCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGAAAAAACAAAACCTCAGTTTTTATACGCCCCCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCCTCCACAACGAACttg           >  1:344823/1‑149 (MQ=255)                                                                                                                        ggCAGCCTTTTTTT‑TTTGTCCGGTCCCGGCCTC‑TGCGCGGCCTTGCCGGCGAAGTGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgttg        <  6:402927/149‑1 (MQ=255)                                                                                                                            agccttttttATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa                                           <  6:318549/107‑1 (MQ=38)                                                                                                                            agccttttttATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa                                           >  5:318549/5‑111 (MQ=38)                                                                                                                               cttttttATTTGTCCTGTGCCGGCCTC‑TTCGCGGACTTGCGTGGTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGccacc  <  1:402582/148‑1 (MQ=255)                                                                                                                               cttttttATTTGCCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACttgtt         <  3:264291/141‑1 (MQ=255)                                                                                                                                 tttttATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcca                      >  7:294352/1‑127 (MQ=255)                                                                                                                                 tttttATTTGTCCTGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATcca                      <  8:294352/127‑1 (MQ=255)                                                                                                                                             ||                                                                                                                                       CAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTC‑TTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACC  >  NC_002947/4740679‑4740953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑