| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,480 | 2 bp→AC | intergenic (+57/‑106) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,480 | 0 | C | A | 97.8% | 124.1 / ‑4.9 | 45 | intergenic (+57/‑107) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (21/23); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01 | |||||||||||
| * | NC_002947 | 1,499,481 | 0 | A | C | 97.8% | 128.0 / ‑6.5 | 45 | intergenic (+58/‑106) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (21/23); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGT‑T‑TCGGGCA‑C‑G‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCA > NC_002947/1499339‑1499613 || cGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCtt > 3:201620/1‑149 (MQ=255) gACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCttt > 1:228494/1‑149 (MQ=255) aCGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTg < 2:292632/149‑1 (MQ=255) cGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGc < 1:379445/149‑1 (MQ=255) cGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgtg < 4:201620/149‑1 (MQ=255) gCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgtgt < 3:390976/149‑1 (MQ=255) cAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgtgt > 4:310397/1‑148 (MQ=255) aGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGgtgt < 2:228494/147‑1 (MQ=255) ttCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCCCGGTGCATGGCACC‑GGCGTTGCCGGTGt‑tagc > 2:7079/1‑146 (MQ=38) cGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGt‑tcgcgg > 6:437357/1‑144 (MQ=255) gACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGCAACAGGCGCCGCCGTGCATGGCACC‑GGGGTTGCCGGGGt‑t‑taacgg > 1:146222/1‑144 (MQ=38) aCAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGt‑tcgcgggc < 3:219654/149‑8 (MQ=255) aaCGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑c < 1:7079/149‑1 (MQ=37) gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGt‑tcgc > 4:10981/1‑137 (MQ=255) gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGt‑tcgc < 3:10981/140‑4 (MQ=255) gCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑c‑g‑‑cc > 2:97398/1‑149 (MQ=37) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCCGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGt‑tcgcgg < 7:221676/140‑6 (MQ=37) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGt‑tcgcgg > 8:221676/1‑135 (MQ=255) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑c‑g‑‑cccg > 7:283673/1‑147 (MQ=25) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAAAAGGCGCCCCGGTGCATGGCACC‑CGCGTTGCCCGTGT‑TAGCGGGCA‑A‑GC‑CCGCTCCCACAGACAGAtt > 8:245191/1‑149 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCttt > 3:376757/1‑107 (MQ=255) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCttt < 4:376757/107‑1 (MQ=255) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGt‑tcgcgg > 6:280633/1‑116 (MQ=255) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGt‑tcgcgg < 5:280633/121‑6 (MQ=255) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCg < 8:283673/149‑1 (MQ=21) ccTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCCTGCCCCCGAAACACGCGCCGCGGTGCAAGGCACC‑CGCGTTGACCGTGT‑TAGCGGGCACC‑C‑‑CCCCACCCACAGACAGAATGACATTAAGCg > 8:380041/1‑149 (MQ=255) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCCCAGCCTGCTTGCCATTAAGCGGGCCTa > 5:317267/1‑149 (MQ=21) aaGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATc < 7:380041/149‑1 (MQ=21) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCAtt < 1:356361/124‑1 (MQ=18) cacaGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCCGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCCCTCCCACAGCCGGAATGCCATTAAGCGGGCCTATCGCGACAGTGACg > 7:127304/1‑149 (MQ=18) cagctgGACTGAGGCTGCCAAAGGGTGGGAGC‑GGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGc < 6:317267/149‑1 (MQ=21) gcagGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGGGTTGCCGGTGT‑TAGCGGGCC‑C‑GC‑CCGGGCCCACAGACTGCTTGCCATTAAGCGGGGCTATCGCTACAGTGACGGccc > 6:204379/1‑149 (MQ=21) cagGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCg > 5:133090/1‑149 (MQ=21) agGACTGAGGCTGCCAAACGGTGGGAGCGGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATTGCACC‑GGCGTTGCCGGTGT‑TAGCGGGCC‑C‑GC‑CCGCTCCCACAGACTGATTTGCATTAATCGGGCCTATCGCTACACTGAAGGcccc > 1:240375/1‑148 (MQ=255) ggACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtt < 3:310397/149‑1 (MQ=21) ggACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtt < 1:97398/149‑1 (MQ=21) ccAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCt < 5:437357/148‑1 (MQ=21) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg < 6:353754/130‑1 (MQ=21) gggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgttg > 5:353754/1‑130 (MQ=21) tGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGTCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTccc < 2:280110/149‑1 (MQ=25) ccccGCTAAACCGCCCCCGCGGTG‑AAGGCCACGGGCTTTGCGGTTGT‑C‑CGCGGCA‑A‑GGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 3:221257/148‑1 (MQ=255) cccGCGAAACTGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGTGC‑GCGGGCA‑CGG‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGAGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCt < 2:146222/148‑1 (MQ=37) aaaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACAGATTGCCATTAAGCGGGCCTATCGCTa < 4:22372/94‑1 (MQ=17) aaaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACAGATTGCCATTAAGCGGGCCTATCGCTa > 3:22372/1‑94 (MQ=17) cgcgGTGCATGGCACC‑GGCTTTGCCGGTGT‑TCGCGGGCA‑C‑GC‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCa < 2:240375/149‑1 (MQ=37) || CGACGGGCCGCAGTTCGACAACGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGC‑GGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTGT‑T‑TCGGGCA‑C‑G‑‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCA > NC_002947/1499339‑1499613 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |