Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,151,718	Δ1 bp	7.5%	coding (206/462 nt)	PP_5696 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,151,718	0	G	.	7.5%	206.4 / 10.7	67	coding (206/462 nt)	PP_5696	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/30);  new base . (2/3);  total (33/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

GACTGCGGCTTCTGGAGCGACCGCACCGACAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTGGATTACAGGT  >  NC_002947/5151571‑5151864                                                                                                                                                    |                                                                                                                                                    gACTGCGGCTTCTGGAGCGACCGCACCGACAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAg                                                                                                                                                     <  3:13017/148‑1 (MQ=255)      cGGCTTCTGGAGCGACCGCACCGACAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCAt                                                                                                                                                 <  3:188121/147‑1 (MQ=255)         cTTCTGGAGCGACCGCACCGACAGCTCGGGTGTGATCACCAAACTGTTCTCAGCGGTCAGAATCAAAATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTc                                                                                                                                           >  1:108090/1‑149 (MQ=255)               gAGCGACCGCACCGACAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTg                                                                                                                                     <  1:213072/149‑1 (MQ=255)               gAGCGACCGCACCGACAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAA‑CCATGGCTTCGGa                                                                                                                                        <  6:333389/146‑1 (MQ=255)                  cGACCGCACCGACAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCTACATGGCCGCGTTGGTGTTGGGCTTGATCTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCGGAAGCCATGG‑TTCGGATTGAg                                                                                                                                   <  8:91696/148‑1 (MQ=255)                    aCCGCACCGACAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTg                                                                                                                                >  6:41968/1‑149 (MQ=255)                           cGACAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAg                                                                                                                                   >  6:296068/1‑139 (MQ=255)                           cGACAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAg                                                                                                                                   <  5:296068/139‑1 (MQ=255)                             aCAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGa                                                                                                                       <  4:256435/149‑1 (MQ=255)                              cAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAg                                                                                                                      <  1:243416/149‑1 (MQ=255)                                  tCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGTACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCt                                                                                                                  >  8:105543/1‑149 (MQ=255)                                          gATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCaag                                                                                                           >  1:15041/1‑148 (MQ=255)                                          gATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCaag                                                                                                           >  2:297384/1‑148 (MQ=255)                                           aTCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGt                                                                                                                <  7:229235/142‑1 (MQ=255)                                           aTCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGt                                                                                                                >  8:229235/1‑142 (MQ=255)                                              tcCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGAtt                                                                                                                                      <  4:107101/117‑1 (MQ=255)                                              tcCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGAtt                                                                                                                                      >  3:107101/1‑117 (MQ=255)                                              tcCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAA‑CCATGGCTTCGGacg                                                                                                                                      <  5:342740/117‑3 (MQ=255)                                              tcCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAA‑CCATGGCTTCGGacg                                                                                                                                      >  6:342740/1‑115 (MQ=255)                                                cAAACTGTTATCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGgca                                                                                                     <  8:263556/148‑1 (MQ=255)                                                         ctcAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGc                                                                                            <  7:255770/148‑1 (MQ=255)                                                            aGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCtt                                                                                          <  5:41968/147‑1 (MQ=255)                                                             gCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTg                                                                                         >  3:336728/1‑147 (MQ=255)                                                              cGGTCAGAATCAACATGGCCGCGTTGGGGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAg                                                                                      <  2:296055/149‑1 (MQ=255)                                                                       tgatcATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAATCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCATGAAGGCATGCGTCGGc                                                                                            <  8:24411/130‑1 (MQ=255)                                                                       tCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCACCACACGCATAGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCAGTACAAGAAGGCATGCGTCgcc                                                                                            >  7:24411/1‑132 (MQ=255)                                                                         aaCATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCTAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGgcat                                                                                                    >  6:901/1‑124 (MQ=255)                                                                         aaCATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCTAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGgcat                                                                                                    <  5:901/124‑1 (MQ=255)                                                                         aaCATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCaa                                                                                                            >  8:101418/1‑116 (MQ=255)                                                                         aaCATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCaa                                                                                                            <  7:101418/116‑1 (MQ=255)                                                                          aCATGGCGGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGACGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATggg                                                                           >  7:235610/1‑148 (MQ=255)                                                                          aCATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATggg                                                                           >  2:211275/1‑148 (MQ=255)                                                                                ccGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATc                                                                    <  1:180649/149‑1 (MQ=255)                                                                                    gTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAAt                                                                <  4:336728/149‑1 (MQ=255)                                                                                             gggCTTGAACTCCTCAACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCGTGTTCAAGAAGGCATGCATCGGCTTGTAGGAGACAATGGGGAGCATCAAATAGATTAGc                                                        >  6:342937/1‑148 (MQ=255)                                                                                                cTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCaaaa                                                    >  7:322201/1‑149 (MQ=255)                                                                                                   gtaCTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAAt                                                   <  7:105543/145‑1 (MQ=255)                                                                                                       tcctccACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGgga                                                                         >  1:103861/1‑121 (MQ=255)                                                                                                       tcctccACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGgga                                                                         <  2:103861/121‑1 (MQ=255)                                                                                                       tcctccACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCtt                                             >  3:76792/1‑149 (MQ=255)                                                                                                         ctccACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTtct                                           >  1:168765/1‑149 (MQ=255)                                                                                                         ctccACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTtc                                            <  2:108090/148‑1 (MQ=255)                                                                                                           ccACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTtctc                                          >  4:330234/1‑148 (MQ=255)                                                                                                            cacaCGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCa                                        >  5:254654/1‑149 (MQ=255)                                                                                                                      gCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATTGGGTGGATCAAATAGCTGAGCAAAATGTCCTTCTCAACCGGCTaccc                              >  7:131237/1‑148 (MQ=255)                                                                                                                         aaGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGGTTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTg                           >  5:39578/1‑149 (MQ=255)                                                                                                                         aaGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTg                           <  1:211275/149‑1 (MQ=255)                                                                                                                         aaGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTg                           >  3:82167/1‑149 (MQ=255)                                                                                                                          aGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGc                          <  4:76792/149‑1 (MQ=255)                                                                                                                                    ccTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAg                             >  5:76388/1‑136 (MQ=255)                                                                                                                                    ccTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAg                             <  6:76388/136‑1 (MQ=255)                                                                                                                                     ctctCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTc                                                <  7:139345/116‑1 (MQ=255)                                                                                                                                     ctctCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTc                                                >  8:139345/1‑116 (MQ=255)                                                                                                                                      tctcAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGc                          >  4:85999/1‑137 (MQ=255)                                                                                                                                      tctcAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGc                          <  3:85999/137‑1 (MQ=255)                                                                                                                                       ctcAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAActct             >  1:194723/1‑149 (MQ=255)                                                                                                                                          aGCGTCCGAAGCCCTGG‑TGCGGATTGAGCTGTCACGCCGAGCACTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTGGa          <  8:235610/149‑1 (MQ=255)                                                                                                                                          aGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGATCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAActct             <  1:297384/146‑1 (MQ=255)                                                                                                                                          aGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTg            <  2:15041/147‑1 (MQ=255)                                                                                                                                          aGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTGGa          <  4:82167/149‑1 (MQ=255)                                                                                                                                          aGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTGGa          >  4:325607/1‑149 (MQ=255)                                                                                                                                            cGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTGGAt         >  4:260440/1‑148 (MQ=255)                                                                                                                                            aatCCGAA‑CCATGGCTTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTg            >  8:106433/3‑145 (MQ=255)                                                                                                                                            aatCCGAA‑CCATGGCTTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTg            <  7:106433/143‑1 (MQ=255)                                                                                                                                                  aaGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTGGATTACAGGt  >  3:207040/1‑149 (MQ=255)                                                                                                                                                  aaGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTGGATTACAGGt  <  6:254654/149‑1 (MQ=255)                                                                                                                                                   aGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTGGATTACAGGt  <  4:207040/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GACTGCGGCTTCTGGAGCGACCGCACCGACAGCTCGGGTGTGATCTCCAAACTGTTCTCAGCGGTCAGAATCAACATGGCCGCGTTGGTGATGGGCTTGAACTCCTCCACACGCATCGCCAAGCCGATAAGCCTCTCAGCGTCCGAAGCCATGG‑TTCGGATTGAGCTGTCACGCCGAGCCCTGTTCAAGAAGGCATGCATCGGCTTGTAGGTGACAATGGGGAGGATCAAATAGCTGAGCAAAATGTCCTTCTCCACCGGCTCCCAGTGCTGGTAAAAACTCTGGATTACAGGT  >  NC_002947/5151571‑5151864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑