Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,470,290 Δ1 bp 7.1% coding (1615/1890 nt) mrdA‑II ← transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,470,2900T. 7.1% 167.8 / 7.1 56coding (1615/1890 nt)mrdA‑IItranspeptidase
Reads supporting (aligned to +/- strand):  ref base T (21/31);  new base . (2/2);  total (23/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

GACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTC  >  NC_002947/5470150‑5470431
                                                                                                                                            |                                                                                                                                               
gACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGcc                                                                                                                                           <  2:39050/147‑1 (MQ=255)
       aCCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCg                                                                                                                                >  3:208176/1‑149 (MQ=255)
       aCCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCg                                                                                                                                <  2:204954/149‑1 (MQ=255)
       aCCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGC‑ATTGCCG‑GCaagagcg                                                                                                                                >  7:261889/1‑144 (MQ=255)
        ccGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCgg                                                                                                                               >  1:183767/1‑149 (MQ=255)
         cGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCg‑g                                                                                                                                        <  4:124787/140‑1 (MQ=255)
         cGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCg‑g                                                                                                                                        >  3:124787/1‑140 (MQ=255)
           aaaCCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGC‑ATTGCCG‑GCAAGAGCGGTAc                                                                                                                            <  8:261889/149‑1 (MQ=38)
            aaCCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACt                                                                                                                           <  1:56334/149‑1 (MQ=255)
               cACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTgg                                                                                                                         >  5:18099/1‑148 (MQ=255)
               cACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGC‑ATTGCCG‑GCaagag                                                                                                                                  >  1:149245/1‑136 (MQ=255)
               cACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGC‑ATTGCCG‑GCaagag                                                                                                                                  <  2:149245/139‑4 (MQ=255)
                  gATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTc                                                                                                                     >  7:224318/1‑149 (MQ=255)
                          gggCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCt                                                                                                             <  6:161063/149‑1 (MQ=255)
                          gggCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGGGGCGCTCCGGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCt                                                                                                             <  7:188203/149‑1 (MQ=255)
                           ggCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTg                                                                                                            <  2:276872/149‑1 (MQ=255)
                           ggCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTg                                                                                                            >  1:276872/1‑149 (MQ=255)
                                  gCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTgcg                                                                                                     >  3:250164/1‑149 (MQ=255)
                                        gCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCgg                                                                                                >  6:114123/1‑148 (MQ=255)
                                               cgacgaACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCgg                                                                                        <  2:183767/149‑1 (MQ=255)
                                               cgacgaACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCgg                                                                                        <  4:208176/149‑1 (MQ=255)
                                                 acgaACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGc                                                                                      <  4:250164/149‑1 (MQ=255)
                                                       aGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCGCGCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTtgt                                                                                 <  1:279329/149‑1 (MQ=255)
                                                        gggCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGc                                                                               <  5:114123/149‑1 (MQ=255)
                                                         ggCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCa                                                                              >  4:50187/1‑149 (MQ=255)
                                                              ggTCGCGGTGGCGCTCCTGGAGTTTGTGGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGTTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCAc                                                                          <  4:319500/148‑1 (MQ=255)
                                                                    ggTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTcc                                                                   <  1:214042/149‑1 (MQ=255)
                                                                     gTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACcgcg                                                                                         <  7:116633/126‑1 (MQ=255)
                                                                     gTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACcgcg                                                                                         >  8:116633/1‑126 (MQ=255)
                                                                        gcgcTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTccatgc                                                               <  7:81976/149‑1 (MQ=255)
                                                                          gcTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTccatgcca                                                             <  5:333195/149‑1 (MQ=255)
                                                                            tCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTccatgccatg                                                           >  1:165143/1‑149 (MQ=255)
                                                                              cTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATgg                                                          <  5:78970/148‑1 (MQ=255)
                                                                                       gttgCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTg                                                 <  2:193912/148‑1 (MQ=255)
                                                                                             aTCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGt                                          >  7:41597/1‑149 (MQ=255)
                                                                                                  aCTTCTCGCCCTGCTTGATCGCCACCACCCGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATgg                                                          <  5:72328/128‑1 (MQ=255)
                                                                                                  aCTTCTCGCCCTGCTTGATCGCCACCACCCGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATgg                                                          >  6:72328/1‑128 (MQ=255)
                                                                                                   cTTTTCGCCCTGCTTGATCGCCAGCACCAGGGCGGTACCCCTCTTGCCG‑GCCA‑TGCTGTTCTGGGCTCGGGCAGCTGCCTTGCGCGCGGTACCGCTGGCGTTGTGCATCACCTGTTCCATTCCATGGGTGACCTTGGCCCAGTCGGAc                                     <  8:298154/148‑1 (MQ=255)
                                                                                                    ttCTCGCCCTGCTTGATCGCCACGACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACcgc                                                                                          <  4:347017/94‑1 (MQ=255)
                                                                                                    ttCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACcgc                                                                                          >  3:347017/1‑94 (MQ=255)
                                                                                                    ttCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACtt                                   >  1:282405/1‑149 (MQ=255)
                                                                                                         gCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCAc                              >  2:236794/1‑149 (MQ=255)
                                                                                                           ccTGCTTGATCGCCACCGCCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTCGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTcc                                                                   >  8:246569/1‑110 (MQ=255)
                                                                                                           ccTGCTTGATCGCCACCGCCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTCGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTcc                                                                   <  7:246569/110‑1 (MQ=255)
                                                                                                                  gATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTc                                <  6:305007/138‑1 (MQ=255)
                                                                                                                  gATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTc                                >  5:305007/1‑138 (MQ=255)
                                                                                                                    tCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATgg                                                          <  6:342138/110‑1 (MQ=255)
                                                                                                                    tCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATgg                                                          >  5:342138/1‑110 (MQ=255)
                                                                                                                       ccaccaCCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCt                >  7:29799/1‑149 (MQ=255)
                                                                                                                        caccacCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGGGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCtt               <  5:122524/149‑1 (MQ=255)
                                                                                                                        caccacCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCtt               >  5:105349/1‑149 (MQ=255)
                                                                                                                             ccTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATg          <  8:29799/149‑1 (MQ=255)
                                                                                                                                 ggCGGTACCGCTCTTGCCT‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATgggg       <  2:195227/148‑1 (MQ=255)
                                                                                                                                     gTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTgtc  <  2:9674/149‑1 (MQ=255)
                                                                                                                                     gTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTgtc  <  2:165143/149‑1 (MQ=255)
                                                                                                                                     gTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTgtc  <  8:41597/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                               
GACCATCACCGAAACCACGATTTTCGGGGCTTCGGCTGGGGCAAACGCGACGAACAGGGCATGGTCGCGGTGGCGCTCCTGGAGTTTGTTGCGATCGTACTTCTCGCCCTGCTTGATCGCCACCACCTGGGCGGTACCGCTCTTGCCG‑GCAA‑TGCGGTACTGGGCTCCGGCAGCTGCCTTGCGCGCGGTACCGCGGGCGTTGTGCATCACCTGTTCCATGCCATGGGTGACCTTGGCCCAGTCGGACTTGTCACGCAGTACGATGTCTTCCATGGGGTTGTC  >  NC_002947/5470150‑5470431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑