Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,846,297	Δ1 bp	10.5%	coding (588/1377 nt)	pfeS‑II →	histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,846,297	0	T	.	10.5%	169.3 / 14.0	57	coding (588/1377 nt)	pfeS‑II	histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (26/25);  new base . (3/3);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATGC  >  NC_002947/1846149‑1846445                                                                                                                                                      |                                                                                                                                                      aTCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGAt                                                                                                                                                       >  2:70194/1‑149 (MQ=255)  tCCTGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCAACTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATc                                                                                                                                                      <  5:83015/149‑1 (MQ=255)         cAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑Tc                                                                                                                                              >  6:163463/1‑149 (MQ=255)           gCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCgg                                                                                                                                            <  5:163463/149‑1 (MQ=255)            cACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGc                                                                                                                                           <  6:195926/149‑1 (MQ=255)            cACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCAGGCGGG‑CCGA‑CAGCTGATCGGc                                                                                                                                           >  7:241852/1‑149 (MQ=255)                    gCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTTAACCAACTTCGCGAGCAGGCCAATGCCAGGCGGG‑CCGA‑CAGCTGATCGGCCCGccag                                                                                                                                    <  4:263184/148‑3 (MQ=255)                     cTGTGGCGCATGCTCACCAACGGCCTCCTCCCGGGGCTGTGCACCGTGCTGGTGTGTTTACGCCTGTCCCGCATCCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGTG‑CCGATCAGCTG‑TCGGCCCGCCTGGa                                                                                                                                  <  4:258643/149‑1 (MQ=255)                          gcgcATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCAGGCGGG‑CCGA‑CAGCTGATCGGCCCGCCAggcattg                                                                                                                              <  8:241852/148‑6 (MQ=38)                            gcATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCgcgc                                                                                                                           <  2:279216/149‑1 (MQ=255)                                  tCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATc                                                                                                                     >  8:293498/1‑149 (MQ=255)                                  tCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATc                                                                                                                     >  2:234170/1‑149 (MQ=255)                                        aCGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCg                                                                                                                <  1:70194/149‑1 (MQ=255)                                                            ttCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGaa                                                                                             <  8:224264/147‑1 (MQ=255)                                                                 cTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCTAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGgcgc                                                                                      <  2:290934/149‑1 (MQ=255)                                                                 cTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGgcgc                                                                                      <  6:134378/149‑1 (MQ=255)                                                                        ttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCtt                                                                               >  2:223739/1‑149 (MQ=255)                                                                        ttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCtt                                                                               >  5:131880/1‑149 (MQ=255)                                                                          gtgtgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAAc                                                                                            <  6:84284/134‑1 (MQ=255)                                                                          gtgtgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAAc                                                                                            >  5:84284/1‑134 (MQ=255)                                                                          gtgtgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCttt                                                                              <  7:293498/148‑1 (MQ=255)                                                                            gtgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                           >  2:226672/1‑149 (MQ=255)                                                                             tgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                           >  3:32982/1‑148 (MQ=255)                                                                                 ggCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGAt                                                                      <  1:223739/149‑1 (MQ=255)                                                                                  gCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATg                                                                     >  6:75259/1‑149 (MQ=255)                                                                                               gCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                           <  5:111526/130‑1 (MQ=255)                                                                                               gCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                           >  6:111526/1‑130 (MQ=255)                                                                                                 tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGc                                                        >  2:240758/1‑147 (MQ=255)                                                                                                 tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGc                                                        >  8:120930/1‑147 (MQ=255)                                                                                                 tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCAGCTGc                                                        >  1:123164/1‑147 (MQ=255)                                                                                                 tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCAACTGc                                                        >  1:127193/1‑147 (MQ=255)                                                                                                     cGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCa                                                  >  5:92012/1‑149 (MQ=255)                                                                                                      gTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCAc                                                 >  7:45084/1‑149 (MQ=255)                                                                                                          cGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTg                                             <  6:131880/149‑1 (MQ=255)                                                                                                           gCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGc                                                                   <  4:286114/126‑1 (MQ=255)                                                                                                           gCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGc                                                                   >  3:286114/1‑126 (MQ=255)                                                                                                                 ccAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATg                                       >  5:59455/1‑148 (MQ=255)                                                                                                                    aCTTCGCTAGCTGGCCAATGCCTGGCGGG‑CCTATCAGGTG‑TCGGCCGGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCttt                                                                              <  6:35297/106‑1 (MQ=255)                                                                                                                    aCTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCttt                                                                              >  5:35297/1‑106 (MQ=255)                                                                                                                    aCTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATgcagc                                   <  5:75259/149‑1 (MQ=255)                                                                                                                     cTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATgcagca                                  <  1:240758/149‑1 (MQ=255)                                                                                                                          cgAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGt                             <  4:32982/149‑1 (MQ=255)                                                                                                                              cAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTtgct                         <  6:92012/149‑1 (MQ=255)                                                                                                                                   cAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGcgcg                    >  7:264274/1‑149 (MQ=255)                                                                                                                                   attggCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTgcgc                     >  4:221335/5‑148 (MQ=255)                                                                                                                                         caggCGGG‑CCGA‑CAGCTGATCGGCCCGCCAGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAgg                                                    <  1:194012/109‑1 (MQ=38)                                                                                                                                         caggCGGG‑CCGA‑CAGCTGATCGGCCCGCCAGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAgg                                                    >  2:194012/3‑111 (MQ=38)                                                                                                                                         cTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGt              <  2:127193/149‑1 (MQ=255)                                                                                                                                         cTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGt              <  2:123164/149‑1 (MQ=255)                                                                                                                                            gCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTcc            >  8:242488/1‑148 (MQ=255)                                                                                                                                            gCGGG‑CCGA‑CAGCTGATCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGTGCGACCTGTcc            >  1:209257/1‑148 (MQ=255)                                                                                                                                              ggg‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCAc         >  5:94440/1‑149 (MQ=255)                                                                                                                                                g‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGCCCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAACtgctgctgcat                     >  5:146673/1‑133 (MQ=255)                                                                                                                                                g‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAACtgctgctgcat                     <  6:146673/135‑3 (MQ=255)                                                                                                                                                   cGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGaaa     >  7:32143/1‑149 (MQ=255)                                                                                                                                                    gATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAAt    <  7:242488/149‑1 (MQ=255)                                                                                                                                                    gATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAAt    >  6:25785/1‑149 (MQ=255)                                                                                                                                                     aTCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATg   <  1:234170/149‑1 (MQ=255)                                                                                                                                                      tCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGGGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATgc  <  8:45084/149‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                      ATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATGC  >  NC_002947/1846149‑1846445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑