Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,904,244 Δ1 bp 7.5% coding (499/1593 nt) ilvA‑I ← threonine deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,904,2440T. 7.5% 158.5 / 7.2 53coding (499/1593 nt)ilvA‑Ithreonine deaminase
Reads supporting (aligned to +/- strand):  ref base T (25/23);  new base . (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TAGGCGGCGATCCCGGCGATCAAGCCGCCACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCGTGGTGGTGGGCATGACGATGGTTGCCTTCA  >  NC_002947/3904099‑3904390
                                                                                                                                                  |                                                                                                                                                   
tAGGCGGCGATCCCGGCGATCAAGCCGCCACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTcgc                                                                                                                                                  <  8:49813/149‑1 (MQ=255)
        gATCCCGGCGATCAAGCCGCCACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGc                                                                                                                                         >  5:101530/1‑149 (MQ=255)
         aTCCCGGCGATCAAGCCGCCACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCt                                                                                                                                        >  4:292801/1‑149 (MQ=255)
                   tCAAGCCGCCACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGc                                                                                                                              <  2:136142/149‑1 (MQ=255)
                     aaGCCGCCACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGG‑CGCGACCTTCgtg                                                                                                                                        >  1:100609/1‑135 (MQ=255)
                     aaGCCGCCACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGG‑CGCGACCTTCgtg                                                                                                                                        <  2:100609/137‑3 (MQ=255)
                     aaGCCGCCACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGG‑CGCGACCTTCgt                                                                                                                                         <  5:110806/136‑2 (MQ=255)
                     aaGCCGCCACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGG‑CGCGACCTTCgt                                                                                                                                         >  6:110806/1‑135 (MQ=255)
                         cgccACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGt                                                                                                                        >  1:276412/1‑149 (MQ=255)
                             aCCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGt                                                                                                                    <  6:140836/149‑1 (MQ=255)
                             aCCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGt                                                                                                                    >  6:256380/1‑149 (MQ=255)
                              ccGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTg                                                                                                                   <  3:79395/149‑1 (MQ=255)
                               cGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGTGCGCTGACGGAGGATTTCCATGGCGACGGTTCCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CGTTCGCTGTCGGCCAGCTTCAGTGCGTgg                                                                                                                  <  7:7458/149‑1 (MQ=255)
                               cGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTgg                                                                                                                  <  6:101530/149‑1 (MQ=255)
                                     cGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAgc                                                                                                            <  3:292801/149‑1 (MQ=255)
                                      gggCACAAAGCTCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAgcg                                                                                                           <  8:164713/149‑1 (MQ=255)
                                        gCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGc                                                                                                                                         <  5:102596/117‑1 (MQ=255)
                                        gCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGc                                                                                                                                         >  6:102596/1‑117 (MQ=255)
                                          acaAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATg                                                                                                       >  3:118232/1‑149 (MQ=255)
                                           caAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGc                                                                                                      <  8:41447/149‑1 (MQ=255)
                                            aaaGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCg                                                                                                     >  6:100230/1‑149 (MQ=255)
                                                 tCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAAGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAg                                                                                                <  4:6165/149‑1 (MQ=255)
                                                     gTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGctct                                                                                            >  1:57730/1‑149 (MQ=255)
                                                         aaCGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGACGCGCTCggc                                                                                        >  6:251140/1‑147 (MQ=255)
                                                         aaCGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCtctc                                                                                           >  3:169682/1‑146 (MQ=255)
                                                         aaCGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCtctc                                                                                           <  4:169682/146‑1 (MQ=255)
                                                          aCGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCa                                                                                       >  1:89888/1‑149 (MQ=255)
                                                                cAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATgcagc                                                                                 <  2:276412/149‑1 (MQ=255)
                                                                            ggaggaTTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCccg                                                                     >  2:253035/1‑149 (MQ=255)
                                                                             gaggaTTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCCGGGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTAGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCccg                                                                     >  1:255978/1‑149 (MQ=255)
                                                                              aggaTTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCccgcc                                                                   >  7:95894/1‑149 (MQ=255)
                                                                                       aTGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAacg                                                          >  2:57488/1‑149 (MQ=255)
                                                                                        tGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAacg                                                          >  7:302885/1‑148 (MQ=255)
                                                                                           ccACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTgg                                                                                                                  >  8:136724/1‑89 (MQ=255)
                                                                                           ccACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTgg                                                                                                                  <  7:136724/89‑1 (MQ=255)
                                                                                            cACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAacgcacgc                                                     >  1:60268/1‑149 (MQ=255)
                                                                                               ggTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAacgcac                                                       <  1:24827/144‑1 (MQ=255)
                                                                                               ggTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAacgcac                                                       >  2:24827/1‑144 (MQ=255)
                                                                                                    ccTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACt                                             <  2:57730/149‑1 (MQ=255)
                                                                                                     cTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACtt                                            >  4:98111/1‑149 (MQ=255)
                                                                                                        cccGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTc                                          <  1:57488/148‑1 (MQ=255)
                                                                                                            gCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGa                                     >  6:345606/1‑149 (MQ=255)
                                                                                                              gATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATg                                   <  2:89888/149‑1 (MQ=255)
                                                                                                              gATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATg                                   <  2:60268/149‑1 (MQ=255)
                                                                                                                   caTCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCgtg                              <  8:302885/149‑1 (MQ=255)
                                                                                                                           gTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCGtggtggtgg                       <  4:77467/149‑1 (MQ=255)
                                                                                                                           gTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCGtggtggtgg                       <  1:253035/149‑1 (MQ=255)
                                                                                                                                 gAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCGTGGTGGTGGGCATGa                 >  4:264904/1‑149 (MQ=255)
                                                                                                                                      gTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCGTGGTGGTGGGCATGACGATg            >  2:130102/1‑149 (MQ=255)
                                                                                                                                           aCGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCGTGGTGGTGGGCATGACGATGGTTGc       >  6:271333/1‑149 (MQ=255)
                                                                                                                                             gAAGGGCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCGTGGTGGTGGGCATGACGATGGTTGCCt     <  8:126594/149‑1 (MQ=255)
                                                                                                                                               aGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCGTGGTGGTGGGCATGACGATGGTTGCCtt    >  4:180635/1‑148 (MQ=255)
                                                                                                                                                ggTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCGTGGTGGTGGGCATGACGATGGTTGCCTTCa  <  3:98111/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TAGGCGGCGATCCCGGCGATCAAGCCGCCACCGCCCACGGGCACAAAGATCGCGTCCAACGCCCCAGGGCGCTGACGGAGGATTTCCATGGCCACGGTACCCTGCCCGGCGATCACATCC‑GGGTCATCGAACGGTGGCACGAAGGTCGCG‑CCTTCGCTGTCGGCCAGCTTCAGTGCGTGGGTCAGCGCATGCGGGAAGCTCTCGCCATGCAGCACCACATGCCCGCCGCGCGAACGCACGCCTTCCACTTTCAACGATGGCGTGGTGGTGGGCATGACGATGGTTGCCTTCA  >  NC_002947/3904099‑3904390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑