Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	428,858:1	+C	6.2%	coding (149/957 nt)	PP_0351 ←	transmembrane sensor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	428,858	1	.	C	6.2%	259.5 / 10.6	65	coding (149/957 nt)	PP_0351	transmembrane sensor
Reads supporting (aligned to +/- strand):  ref base . (27/34);  new base C (2/2);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

CATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGAGGTTGC  >  NC_002947/428711‑429002                                                                                                                                                     |                                                                                                                                                  cATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑g                                                                                                                                                  <  1:128744/149‑1 (MQ=255) cATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑g                                                                                                                                                  <  5:128778/149‑1 (MQ=255)  aTGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGCGCT‑gg                                                                                                                                                 >  4:32591/1‑149 (MQ=255)  aTGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑gg                                                                                                                                                 <  4:62127/149‑1 (MQ=255)     ccAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑Ggcgc                                                                                                                                              >  7:84113/1‑149 (MQ=255)         cagcGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCTGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAgg                                                                                                                                          >  6:366602/1‑149 (MQ=255)           gcgcgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCCTGGCGCC‑AGCTCGGCGCCatgga                                                                                                                                        <  7:230575/149‑5 (MQ=255)           gcgcgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCa                                                                                                                                        >  8:305214/1‑149 (MQ=255)           gcgcgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCa                                                                                                                                        >  7:295800/1‑149 (MQ=255)            cgcgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAc                                                                                                                                       <  2:260936/149‑1 (MQ=255)            cgcgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAc                                                                                                                                       <  8:84113/149‑1 (MQ=255)             gcgcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcg                                                                                                                                      >  6:222917/1‑149 (MQ=255)               gcCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGTGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtg                                                                                                                                    >  8:112794/1‑149 (MQ=255)                ccGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGTGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgc                                                                                                                                   <  7:112794/149‑1 (MQ=255)                 cGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgcg                                                                                                                                  >  5:227535/1‑149 (MQ=255)                  gAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgcgt                                                                                                                                 <  3:101659/149‑1 (MQ=255)                   aGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgcgtg                                                                                                                                <  8:295800/149‑1 (MQ=255)                    gCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACgtgcgtgc                                                                                                                               >  6:22679/1‑149 (MQ=255)                    gCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACgtgcgtgc                                                                                                                               <  1:136887/149‑1 (MQ=255)                     cAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACgtgcgtgc                                                                                                                               <  7:305214/148‑1 (MQ=255)                       gAAACGTGCCGGCCAGCCCGCCGCCACCCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGctct                                                                                                                            >  5:178258/1‑149 (MQ=255)                         aaCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTc                                                                                                                          <  6:178258/149‑1 (MQ=255)                         aaCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCGGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTc                                                                                                                          <  5:222917/149‑1 (MQ=255)                          aCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCTGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCg                                                                                                                         >  2:92676/1‑149 (MQ=255)                          aCGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCg                                                                                                                         <  7:287015/149‑1 (MQ=255)                             tGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTg                                                                                                                      >  6:287142/1‑149 (MQ=255)                                  gCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTggcgg                                                                                                                  >  1:350349/1‑148 (MQ=255)                                   ccAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCa                                                                                                                <  8:254282/149‑1 (MQ=255)                                    cAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCAt                                                                                                               <  7:286331/149‑1 (MQ=255)                                        ccgccgCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgc                                                                                                                                   <  8:314594/125‑1 (MQ=255)                                        ccgccgCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCAcgtgc                                                                                                                                   >  7:314594/1‑125 (MQ=255)                                              ccAACCTTGCCACTGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CAt                                                                                                    <  3:32591/149‑1 (MQ=255)                                               cAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATg                                                                                                   <  4:105651/149‑1 (MQ=255)                                                aaCCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATg                                                                                                   >  2:265806/1‑148 (MQ=255)                                                  ccTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAAt                                                                                                >  3:60722/1‑149 (MQ=255)                                                       ccAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGtt                                                                                           <  8:208305/149‑1 (MQ=255)                                                       ccAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGtt                                                                                           >  7:128042/1‑149 (MQ=255)                                                                cgccgcAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTtcat                                                                                   >  1:330649/1‑148 (MQ=255)                                                                  ccgcAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAg                                                                                <  8:128042/149‑1 (MQ=255)                                                                       ggCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTg                                                                           <  7:251788/149‑1 (MQ=255)                                                                             cgcgcgATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGGGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCgctg                                                                     >  5:247285/1‑149 (MQ=255)                                                                             cgcgcgATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCgctg                                                                     <  2:350349/149‑1 (MQ=255)                                                                                gcgATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGctc                                                                  <  2:330649/149‑1 (MQ=255)                                                                                    ttGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGc                                                              <  4:60722/149‑1 (MQ=255)                                                                                       cagcagCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCaa                                                           <  2:31649/149‑1 (MQ=255)                                                                                           agcGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCt                                                       <  5:366602/149‑1 (MQ=255)                                                                                              gcCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCGCCATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGa                                                     <  1:265806/149‑1 (MQ=255)                                                                                                cTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGa                                                     <  3:352331/146‑1 (MQ=255)                                                                                                     gTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAg                                             <  1:196396/149‑1 (MQ=255)                                                                                                             ccAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATcgc                                     >  3:65020/1‑149 (MQ=255)                                                                                                                      gcgcTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCt                                                       >  6:23163/1‑122 (MQ=255)                                                                                                                      gcgcTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCt                                                       <  5:23163/122‑1 (MQ=255)                                                                                                                       cgcTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAg                            >  3:296941/1‑148 (MQ=255)                                                                                                                            aCGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCg                      >  3:66644/1‑149 (MQ=255)                                                                                                                              gcgTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCATGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCggg                    >  8:203026/1‑149 (MQ=255)                                                                                                                                gTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGAc                  >  7:135264/1‑149 (MQ=255)                                                                                                                                  acgtgcctGGCGCC‑AGCTCGGCGCCATGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACg                 >  5:130791/8‑148 (MQ=255)                                                                                                                                       cATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAAcc            <  8:219040/148‑1 (MQ=255)                                                                                                                                        ctGGCGCC‑AGCTCGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAg                                                    <  2:60147/106‑1 (MQ=255)                                                                                                                                        ctGGCGCC‑AGCTCGGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAg                                                    >  1:60147/2‑107 (MQ=255)                                                                                                                                         tGGCGCCGGGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCggg         <  6:247285/149‑1 (MQ=255)                                                                                                                                         tGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCACTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCgg          >  2:106577/1‑148 (MQ=255)                                                                                                                                          ggCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCCGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGa        <  4:12397/149‑1 (MQ=255)                                                                                                                                           gcgcTGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGa        <  1:92676/148‑1 (MQ=255)                                                                                                                                           gcgcCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGa        <  4:65020/148‑1 (MQ=255)                                                                                                                                           gcgcCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGAg       <  5:287142/149‑1 (MQ=255)                                                                                                                                                gAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGGTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGAGGTTGc  >  5:263972/1‑149 (MQ=255)                                                                                                                                                   cT‑GGCGCCAGGCACGTGCGTGCTCTTCACTGGCGGCATGCCAGCG‑CATGAATTCGTTg                                                                                          <  3:211465/58‑1 (MQ=255)                                                                                                                                                   cT‑GGCGCCAGGCACGTGCGTGCTCTTCACTGGCGGCATGCCAGCG‑CATGAATTCGTTg                                                                                          >  4:211465/1‑58 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CATGCCAGCAGCGCGCCGAGCAGAAACGTGCCGGCCAGCCCGCCGCCAACCTTGCCAATGCGTCGCCGCAGGCTGGCGCGCGATTGCAGCAGCGCCTGGCGTGCCGGGCCAGCTGCCGCGCTAACGCGTTGGTCCATGGCGCCGAGCT‑GGCGCCAGGCACGTGCGTGCTCTTCGCTGGCGGCATGCCAGCG‑CATGAATTCGTTGCGTTCATCAGGCGTGCCGCTGCTCTCGCCAAGGCTGAGTTTCCAGGCAATCGCGGCTTCCAGGACCCGGGACGAAACCGGGAGGTTGC  >  NC_002947/428711‑429002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑