Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,726,804:1	+G	12.3%	coding (162/2574 nt)	opgH ←	glucans biosynthesis glucosyltransferase H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,726,804	1	.	G	12.3%	217.7 / 26.9	65	coding (162/2574 nt)	opgH	glucans biosynthesis glucosyltransferase H
Reads supporting (aligned to +/- strand):  ref base . (27/30);  new base G (3/5);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 7.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01

GCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTGCGC  >  NC_002947/5726662‑5726952                                                                                                                                                |                                                                                                                                                     gcCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACg                                                                                                                                                >  6:153908/1‑149 (MQ=255)    aTGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGcc                                                                                                                                             >  4:130239/1‑149 (MQ=255)       gCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCg                                                                                                                                          <  2:214441/149‑1 (MQ=255)        cTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCTCAGGCGGCCGGTGCCGTCGACGCCGAGCTTTTCGGGTTCTTCCAGCTCGGCGGCACTGGCCACGCTCTGGCGCGGGCCCACCGAGGCC‑TGCACGGCGTCgg                                                                                                                                         <  6:23423/149‑1 (MQ=255)        cTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCCGTGCA‑GGCCTCgg                                                                                                                                         <  7:93747/149‑1 (MQ=255)        cTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCgg                                                                                                                                         <  5:371885/149‑1 (MQ=255)         tCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCgg                                                                                                                                         >  7:145222/1‑148 (MQ=255)           gggCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCGCCGAGGCCGTGCA‑GGCCTCggtgg                                                                                                                                      <  1:36674/149‑4 (MQ=255)            ggCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCaga                                                                                                                                     <  5:61027/149‑1 (MQ=255)               aCGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCt                                                                                                                                  >  8:96256/1‑149 (MQ=255)               aCGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑GGCCCGGCCTCGGCAGAGcg                                                                                                                                  >  2:314377/1‑148 (MQ=255)                         tGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCACGCGCGGGCACACCCAAGCC‑AGCAAGGCCTCCGCAGAGATGGCCGCCagg                                                                                                                        >  4:231022/1‑149 (MQ=255)                            gTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTggc                                                                                                                               <  5:325338/139‑1 (MQ=255)                            gTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTggc                                                                                                                               >  6:325338/1‑139 (MQ=255)                                gATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGgcc                                                                                                                  <  1:88074/148‑1 (MQ=255)                                  tCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGgccgc                                                                                                                >  1:316609/1‑148 (MQ=255)                                    ggcggGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGgccgc                                                                                                                <  4:142590/146‑1 (MQ=255)                                    ggcggGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGgccgc                                                                                                                >  3:142590/1‑146 (MQ=255)                                     gcggGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCCGTGCA‑GGCCTCggt                                                                                                                                        <  3:140712/121‑2 (MQ=255)                                     gcggGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCCGTGCA‑GGCCTCggt                                                                                                                                        >  4:140712/1‑120 (MQ=255)                                        gggCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGt                                                                                                         <  1:314377/149‑1 (MQ=255)                                          gCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCGCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTg                                                                                                       >  7:279403/1‑149 (MQ=255)                                           cGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGGTGGCCGCCCAGCGTTgg                                                                                                      >  3:315204/1‑149 (MQ=255)                                            gATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCCAGCGTTGgt                                                                                                     >  3:131850/1‑149 (MQ=255)                                             aTCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGgtg                                                                                                    >  6:355463/1‑149 (MQ=255)                                                ttCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGTGGCCGGGTTGGCCGCCAAGCGTTGGTGTAg                                                                                                 >  7:29324/1‑149 (MQ=255)                                                  cAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGgtgt                                                                                                   <  7:96256/145‑1 (MQ=255)                                                  cAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAg                                                                                                 <  6:6779/147‑1 (MQ=255)                                                    ggCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCGCCGAGGCCGTGCA‑GGCCTCggtgg                                                                                                                                      >  1:122588/1‑105 (MQ=255)                                                    ggCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCGCCGAGGCCGTGCA‑GGCCTCggtgg                                                                                                                                      <  2:122588/108‑4 (MQ=255)                                                    ggCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCa                                                                                             <  3:130239/149‑1 (MQ=255)                                                     gCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGt                                                                                                         <  3:178382/136‑1 (MQ=255)                                                     gCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑AGCCCGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGGTGGTGTAGcgaa                                                                                             >  2:320365/1‑145 (MQ=255)                                                      cacaGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACt                                                                                           <  2:145889/149‑1 (MQ=255)                                                          ggcggcCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAAc                                                                                       <  4:313009/149‑1 (MQ=255)                                                            cggcCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGa                                                                                     <  8:249527/149‑1 (MQ=255)                                                             ggcCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAg                                                                                    <  8:145222/149‑1 (MQ=255)                                                             ggcCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAg                                                                                    <  5:93166/149‑1 (MQ=255)                                                              gcCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAgg                                                                                   <  1:320365/149‑1 (MQ=255)                                                                   tGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAAc                                                                                       >  6:192702/1‑140 (MQ=255)                                                                   tGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAAc                                                                                       <  5:192702/140‑1 (MQ=255)                                                                      cGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTg                                                                           >  7:177120/1‑149 (MQ=255)                                                                        tCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGc                                                                         <  5:355463/149‑1 (MQ=255)                                                                               cGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCa                                                                  >  1:271402/1‑149 (MQ=255)                                                                                gAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAg                                                                 >  4:294977/1‑149 (MQ=255)                                                                                   cATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGccc                                                              >  7:36107/1‑149 (MQ=255)                                                                                     tttCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCgc                                                            <  2:316609/149‑1 (MQ=255)                                                                                       tCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCgct                                                           <  2:271402/148‑1 (MQ=255)                                                                                       tCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCgct                                                           <  4:131850/148‑1 (MQ=255)                                                                                              cttcCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCtcgtc                                                    <  2:206457/148‑1 (MQ=255)                                                                                                    gCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGt                                             <  8:36107/149‑1 (MQ=255)                                                                                                     cTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTg                                            <  3:231022/149‑1 (MQ=255)                                                                                                               cTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGc                                  >  8:89465/1‑149 (MQ=255)                                                                                                                tGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGc                                  >  5:69345/1‑148 (MQ=255)                                                                                                                             gcgcGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCaa                    <  7:89465/149‑1 (MQ=255)                                                                                                                                     ccACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCt                                                                            <  7:60653/85‑1 (MQ=255)                                                                                                                                     ccACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCt                                                                            >  8:60653/1‑85 (MQ=255)                                                                                                                                     ccACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTcc            >  1:113381/1‑149 (MQ=255)                                                                                                                                     ccACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGCTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTc             >  1:307085/1‑148 (MQ=255)                                                                                                                                       aCCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGc                  >  4:298095/1‑141 (MQ=255)                                                                                                                                       aCCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGc                  <  3:181505/141‑1 (MQ=255)                                                                                                                                       aCCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGc                  >  4:181505/1‑141 (MQ=255)                                                                                                                                       aCCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGc                  <  3:298095/141‑1 (MQ=255)                                                                                                                                        ccGAGGCCGTGCA‑GGCCTCGGTAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGc                  <  4:294501/140‑1 (MQ=255)                                                                                                                                        ccGAGGCCGTGCA‑GGCCTCGGTAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGc                  >  3:294501/1‑140 (MQ=255)                                                                                                                                               c‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTgcgc  >  5:3537/1‑149 (MQ=255)                                                                                                                                               c‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTgcgc  <  1:351886/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                     GCCATGGCTCGGGCACGACCTTGGTGCGTTTGATCGGCGGGGCGATCTTCAGGCACAGGCGGCCGCTGCCGTCGACGCCGAGCATTTCGGCTTCTTCCAGCTCGGCGGCGCTGCCCACGGTCAGGCGCGGGCCCACCGAGGCC‑TGCACGGCCTCGGCAGAGCTGGCGGCCGGGTTGGCCGCCAAGCGTTGGTGTAGCTCACTGAACGAGGTGCAGCTGGCAAGCTCAGCCCGCTGCTCGTCGCTCAGTGGTAGGTGGGCCAGGTACTCGCCAAGCGATTCCGGCCTTGCGC  >  NC_002947/5726662‑5726952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑