| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 503,837:1 | +C | 8.2% | intergenic (+40/‑111) | PP_0414 → / → rpe | polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 503,837 | 1 | . | C | 8.2% | 188.8 / 11.8 | 49 | intergenic (+40/‑111) | PP_0414/rpe | polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base . (20/25); new base C (2/2); total (22/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01 | |||||||||||
GCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCC > NC_002947/503693‑503970 | gCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑Gaa < 4:156033/148‑1 (MQ=255) ccGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑Gaa > 4:346421/1‑147 (MQ=255) cgcgGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTGCCGGCCTCTTC‑CGGCTTTAGCCGCGAagag < 7:324833/149‑1 (MQ=39) ccTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAgg < 5:129728/149‑1 (MQ=255) gCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCgg < 8:143660/139‑1 (MQ=255) gCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCgg > 7:143660/1‑139 (MQ=255) gCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGt < 3:336957/148‑1 (MQ=255) gCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGt < 3:346421/148‑1 (MQ=255) cGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc < 3:319752/148‑1 (MQ=255) cGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc < 8:250424/148‑1 (MQ=255) ctcGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGaca < 2:279017/148‑1 (MQ=255) ggTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCg > 6:217834/1‑113 (MQ=255) ggTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCg < 5:217834/113‑1 (MQ=255) gttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAgg < 4:289274/114‑1 (MQ=37) gttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTGCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAgg > 3:289274/1‑114 (MQ=37) gttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGAt < 2:236285/148‑1 (MQ=255) ttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGTGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTTAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAc < 1:89136/149‑1 (MQ=255) ttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAc < 3:344288/149‑1 (MQ=255) ctgctgACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCaa > 4:107253/1‑149 (MQ=255) gACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc < 6:371408/126‑1 (MQ=255) ccTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCt > 4:145724/1‑148 (MQ=255) ttGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTc > 5:75093/1‑149 (MQ=255) ttGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTc < 6:268535/149‑1 (MQ=255) gCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTcc < 4:320617/148‑1 (MQ=255) gCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAAt > 3:97938/1‑149 (MQ=255) gagaAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATgc < 3:107253/149‑1 (MQ=255) gCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATgcgcgc > 4:227813/1‑148 (MQ=255) tGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGa > 3:253219/1‑132 (MQ=255) tGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGa < 4:253219/132‑1 (MQ=255) tGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCAc > 7:261838/1‑149 (MQ=255) tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTAACAATGCGCGCCACCTGGAAt > 2:294216/1‑149 (MQ=255) tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTACGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGAGTTGGTCGACAACAATTGATACCAATCAG‑CCTACATCGCTTTACCTGAGTTACAATGCGCGCCACCGAGAAt > 6:153253/1‑149 (MQ=255) aaCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACCCCAAATGATACCAATCAG‑CCTGCATCGCTTTCCCTGAGTTAGATAGCGCGCCACCGTGAAttt > 8:137000/1‑149 (MQ=255) aaCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt > 2:256071/1‑149 (MQ=255) aaCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAtt < 3:145724/148‑1 (MQ=255) aCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GTAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt < 1:256071/149‑1 (MQ=255) cGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAAc < 4:97938/149‑1 (MQ=255) atgGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGt < 4:265428/143‑1 (MQ=255) atgGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGt > 3:265428/1‑143 (MQ=255) ttGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt < 4:116125/128‑1 (MQ=255) ttGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt > 3:116125/1‑128 (MQ=255) cTGTACAGGCCTCTTCGCGGCTAAAGCCG‑GATGAGGCCGGAACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTACCCTGAGTTACAATGCGCGCCAACGTGAATTTGCCAACAGGTGCGCCATGCAgaca > 7:174312/1‑145 (MQ=255) tgtgCCGGCCTCTTC‑CGGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTa > 2:27060/1‑149 (MQ=38) cGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACg > 1:13958/1‑146 (MQ=255) cGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACg < 2:13958/146‑1 (MQ=255) ctctTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTa < 1:70754/140‑1 (MQ=255) ctctTCGCGCCTAACGCCG‑GAAGACGCCGGCACAGGATGCGATGGTCGACACCAATTCATACCAATCAG‑CCATCATCGCATTCCCTGAGTTACAATACGCCCCACCGTGAATTTGCCAACAGCATCGCCATGCAGCCCTa > 2:70754/1‑140 (MQ=255) tctTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAA‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGcc > 8:99528/1‑149 (MQ=255) cctTCGCGCCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACa < 8:5595/119‑1 (MQ=255) ttCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGcccc > 3:96679/1‑149 (MQ=255) ttCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGcc < 3:173642/147‑1 (MQ=255) | GCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAG‑CCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACGCTATTGCCCC > NC_002947/503693‑503970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |