Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	644,641:1	+G	7.8%	coding (376/978 nt)	acoA ←	acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	644,641	1	.	G	7.8%	248.4 / 12.5	64	coding (376/978 nt)	acoA	acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha
Reads supporting (aligned to +/- strand):  ref base . (31/28);  new base G (2/3);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTACACGTCCA  >  NC_002947/644493‑644784                                                                                                                                                      |                                                                                                                                                 ttCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGc                                                                                                                                                   <  3:319791/149‑1 (MQ=255)              aGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTgcg                                                                                                                                     >  1:175970/1‑149 (MQ=255)                aTGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCGCACCActggtggcc                                                                                                                                    >  1:320796/1‑146 (MQ=255)                    agggcaggTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCGCACCACTGGtg‑gccgggg                                                                                                                               <  2:320796/149‑3 (MQ=255)                    agggcaggTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTgcgccggcg                                                                                                                               >  4:163921/1‑149 (MQ=255)                     gggcaggTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTgcgccggcgc                                                                                                                              <  2:175970/149‑1 (MQ=255)                            ttCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGTATGCCCCATCGCCGAAGAAGGCTACCGACACGTAATCGCGGCCCTTCAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGAGGTGCGCCGGCGCcgacga                                                                                                                        >  8:192076/1‑148 (MQ=255)                                   tCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGGCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTg                                                                                                               <  1:295541/149‑1 (MQ=255)                                          cATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCAcc                                                                                                          >  2:203443/1‑147 (MQ=255)                                          cATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATAGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCCCCGACGA‑TACCGTTGGCACCGa                                                                                                        >  2:8502/1‑149 (MQ=255)                                                   ggCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCGCACCACTGGtg‑gccggggcg                                                                                                                             <  1:267920/120‑2 (MQ=255)                                                   ggCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCGCACCACTGGtg‑gccggggcg                                                                                                                             <  3:136243/120‑2 (MQ=255)                                                   ggCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGCGCACCACTGGtg‑gccggggcg                                                                                                                             >  2:267920/1‑119 (MQ=255)                                                    gCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCtt                                                                                              >  3:237423/1‑149 (MQ=255)                                                     ccTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCttt                                                                                             >  3:18600/1‑149 (MQ=255)                                                       tCGAATACGGCGCCCTCGTTTGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAgtggt                                                                                                                                        >  6:287529/1‑105 (MQ=255)                                                       tCGAATACGGCGCCCTCGTTTGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAgtggt                                                                                                                                        <  5:287529/105‑1 (MQ=255)                                                       tCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTc                                                                                           >  7:295921/1‑149 (MQ=255)                                                          aaTACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAg                                                                                        >  1:151901/1‑149 (MQ=255)                                                           aTACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAgg                                                                                       <  5:294723/149‑1 (MQ=255)                                                                gcgcCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCgg                                                                                   >  5:214437/1‑148 (MQ=255)                                                                  gcCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCaa                                                                                <  4:79048/149‑1 (MQ=255)                                                                    ccTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAAtg                                                                              >  5:110252/1‑149 (MQ=255)                                                                    ccTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCATGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAAtg                                                                              >  1:284040/1‑149 (MQ=255)                                                                     cTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAAtgt                                                                             <  4:18600/149‑1 (MQ=255)                                                                         ttGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCAt                                                                         <  6:214437/149‑1 (MQ=255)                                                                         ttGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCAt                                                                         >  7:298307/1‑149 (MQ=255)                                                                         ttGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCAt                                                                         >  1:102092/1‑149 (MQ=255)                                                                            gATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGa                                                                      <  3:339789/149‑1 (MQ=255)                                                                              tGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAgc                                                                    <  4:237423/149‑1 (MQ=255)                                                                                 cccATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCCGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TAACGTTGGCCCCGCGGCTGCCCGTTTCCAGGTCGGGAAGGTGGATCGcgccgc                                                                 >  6:100129/1‑149 (MQ=255)                                                                                 cccATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAgccgc                                                                 >  5:249145/1‑149 (MQ=255)                                                                                 cccATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAgccgc                                                                 >  3:256631/1‑149 (MQ=255)                                                                                 cccATCGCCGAAGAAGGCTACCGACACGTCATAGCGGCCCTTGAGCTTGCCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAgccgc                                                                 >  2:212364/1‑149 (MQ=255)                                                                                  ccATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAAtgt                                                                             >  2:109710/1‑136 (MQ=255)                                                                                  ccATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAAtgt                                                                             <  1:109710/136‑1 (MQ=255)                                                                                          gaagaaGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGa                                                                                                        >  3:275353/1‑101 (MQ=255)                                                                                          gaagaaGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGa                                                                                                        <  4:275353/101‑1 (MQ=255)                                                                                            agaagGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCg                                                      <  5:100129/149‑1 (MQ=255)                                                                                             gaagGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CTCCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGc                                                     <  2:151901/149‑1 (MQ=255)                                                                                               agGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAAAGTGCATCGAGCCGCCTTTGCCACAGcac                                                   <  1:203443/149‑1 (MQ=255)                                                                                                ggCTACCGACACGTCATAGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGATGGCACACAGCATGCCCATTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGcaca                                                  >  6:1753/1‑149 (MQ=255)                                                                                                     ccGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCg                                             <  1:212364/149‑1 (MQ=255)                                                                                                            gTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGtcttct                                      >  3:63768/1‑149 (MQ=255)                                                                                                              cATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGTGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTg                                    >  2:317745/1‑149 (MQ=255)                                                                                                               aTCGCGGCCCTTGAGCTTGGCCGGCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTcttctt                                     <  7:170542/147‑1 (MQ=255)                                                                                                               aTCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGc                                   >  5:363007/1‑149 (MQ=255)                                                                                                                tCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGcc                                  >  5:97900/1‑149 (MQ=255)                                                                                                                  gcgGCCCTGGAGCTTGGCCGCCACCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGCGCATGCCCTTTTCCAGGGCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGt                                <  8:92453/149‑1 (MQ=255)                                                                                                                   cgGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTa                               <  8:298307/149‑1 (MQ=255)                                                                                                                   cgGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTa                               <  2:102092/149‑1 (MQ=255)                                                                                                                           gAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGTCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTAGCCACCGCACACGCCTGTCTTCTTGCCGTAGATCTCg                        <  8:295921/148‑1 (MQ=255)                                                                                                                           gAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACTA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGTCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCg                        <  7:192076/148‑1 (MQ=255)                                                                                                                           gAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCg                        <  1:8502/148‑1 (MQ=255)                                                                                                                            aGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGtc                                          >  6:282644/1‑129 (MQ=255)                                                                                                                            aGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGtc                                          <  5:282644/129‑1 (MQ=255)                                                                                                                            aGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCgg                       <  3:163921/148‑1 (MQ=255)                                                                                                                             gCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGc                      <  6:249145/148‑1 (MQ=255)                                                                                                                                     gccAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGcc             >  5:110746/1‑149 (MQ=255)                                                                                                                                         gcgcCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTa          >  7:110901/1‑148 (MQ=255)                                                                                                                                              gccCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTACACGTc    <  4:63768/149‑1 (MQ=255)                                                                                                                                               ccccGGC‑CACCCGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTACACGTcc   <  6:110746/149‑1 (MQ=255)                                                                                                                                                 ccGGC‑CACCAGTGGTGCGCCGGCGCCGACGATTACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTACACGTCCa  >  1:220300/1‑149 (MQ=255)                                                                                                                                                    gC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGcc                                  >  2:232780/1‑113 (MQ=255)                                                                                                                                                    gC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGcc                                  <  1:232780/113‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                 TTCTCGGCCACGAAGATGCAGGGCAGGTTCATGATCGACGCCATGTTCATGGCCTCGAATACGGCGCCCTCGTTGGATGCCCCATCGCCGAAGAAGGCTACCGACACGTCATCGCGGCCCTTGAGCTTGGCCGCCAGCGCCGCCCCGGC‑CACCAGTGGTGCGCCGGCGCCGACGA‑TACCGTTGGCACCGAGCATGCCCTTTTCCAGGTCGGCAATGTGCATCGAGCCGCCTTTGCCACCGCACACGCCGGTCTTCTTGCCGTAGATCTCGGCCATCATGCCGTACACGTCCA  >  NC_002947/644493‑644784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑