| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,126,645:1 | +C | 100% | intergenic (‑99/+50) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 82.9% | 94.9 / 15.9 | 35 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (6/0); new base C (11/18); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAA‑C‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAG > NC_002947/1126498‑1126785 | tGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTTACCGCGAATGCGGAGGTATAGCCCACATGGCATT‑CGC‑GGG‑Gaaaa > 5:282168/1‑148 (MQ=255) gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAA‑c‑cc < 5:341016/149‑2 (MQ=255) aGGGGCCGATGCCGATCTTGAACCGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAA‑c‑ccg < 2:352809/149‑3 (MQ=255) ggCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAA‑c‑ccgctc < 2:77122/149‑6 (MQ=255) cgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAA‑c > 6:65157/1‑134 (MQ=255) cgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAA‑c < 5:65157/134‑1 (MQ=255) ttGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTTACCCCCAAAT‑CGGATGTTTTTCCACCATAGCATTACCC‑GGG‑AAAA‑C‑CCCTCCGAAAAAAAGCCCg > 7:194014/1‑149 (MQ=255) aaCAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGCATGCGGAGGTATAGCCACCATCGCATT‑AGC‑GGG‑TAAACC‑CGCTCCTACACCGAGCCCGccc > 6:269869/1‑148 (MQ=38) gTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGG‑TTTACCC‑CGAATGGGGGGGGTTAGGCCCCCTCGCCTT‑AGCGGGG‑GAAC‑C‑CCCGCCTGCACACAGccccccccccca > 2:31213/1‑147 (MQ=255) tCGAAGACGAGCAGTTACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTAACCCGCTAATGCGGAGGTATAGCCACCATCGCATT‑CGG‑GGT‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCaa < 8:194014/148‑1 (MQ=255) aCGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTAACCGCGAATGCGGAGGTATAGCCACCATAGCATT‑AGC‑GGG‑TAAACC‑CCCTACGACACAAAGCCCCCCACCCAACCCACGt > 5:73916/1‑149 (MQ=255) cGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTg < 5:269869/149‑1 (MQ=255) tCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTTCCCCCGAATTCGGAGGTATAGCCACCATCGCATT‑AGC‑GGG‑TAAACC‑CGCTACTACACACAGCCCGCCACCCAAGCCAGGGGGCg > 4:112426/1‑149 (MQ=255) aCATGTGGACCTCCTGCCAGGTTGTTGGGGGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACACAGCCCGCCACCCAAGCCAGGTGGCGGGGAg < 1:31213/148‑1 (MQ=255) cATGTGCACCTCCTGCCCGGTTCTGGGCTGCACAATGCGCCCGGTAGCCGC‑GGG‑TTAACCCGCGAATGCGCAGGTAAACCCACCACCGCATT‑CGG‑GGT‑AAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAggg < 3:6481/149‑1 (MQ=255) tgtgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTAACCGCGAATGCGGAGGTATAGCCACCATCGCATT‑AGC‑GGG‑GAAA‑CACGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGAGc > 7:341669/1‑148 (MQ=255) tgGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTa < 6:172528/148‑1 (MQ=255) ggACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTAACCGCCAATTCGGTGGTATAGCCACCATAGCATT‑AGC‑GGGTTAAC‑C‑CGCTCCTACACAGAGCCCCCCACCCAACCCAGGTGGCGGGGAGGGCTAt > 3:62145/1‑148 (MQ=255) gACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCa < 5:141315/149‑1 (MQ=255) ctcctcCCTGGTGGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTCCCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGc < 5:206198/149‑1 (MQ=38) ctcctGCCAGGTTGTGGGGGGGACATGGCGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAAGGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGc < 5:211342/149‑1 (MQ=37) tGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTAACCCCGCATGCGGAGGTATAGCCCCCAAAGCCTT‑AGCGGGG‑TAAA‑C‑CGCGCCGACCCAGAGCCCCCCCCCCAACCCACGGGGCGGGGAGGGCAAAAAGGCGtgc > 8:109484/1‑147 (MQ=255) cccggtggtTGGGTGCACATCGAGCCCTGTAGGCGG‑GGT‑TTTCCCCGCGAATGCGGCGGTATAGCCACCATCGCATT‑CGC‑GGG‑AAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTcc < 4:177028/144‑1 (MQ=255) ccAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCt < 1:92890/148‑1 (MQ=255) ttgttgGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGc < 2:205090/149‑1 (MQ=255) gttgGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTTACCCCGAAAGCGGGGGGATAGCCACCATCGCAAT‑AGCGGGG‑AAAA‑C‑CCCGCCGACACACAGCCCCCCCCCCCAGCCCGGGGGGGGGGGGGGCGATGACGCGGCCGGATAg > 8:67913/1‑146 (MQ=255) tGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAg < 1:8498/128‑1 (MQ=255) taCATTGAGCCCTGTAGGGGG‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCtcgatcga < 2:91709/148‑1 (MQ=255) ggCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACAcc < 6:73916/149‑1 (MQ=255) tttACCCGCTAATGCGGAGGTAAAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg < 6:282168/149‑1 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 7:59639/1‑149 (MQ=255) ccGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 3:90196/1‑149 (MQ=255) gcgAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGcc > 3:142495/1‑148 (MQ=255) ggAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGtt < 7:67913/149‑1 (MQ=255) ccaccaTCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGACCCCGTTGTCGACCCgg > 8:333351/1‑149 (MQ=255) ccaccaTCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCgg > 1:20450/1‑149 (MQ=255) ccaTCGCATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGcc > 3:72238/1‑148 (MQ=255) cATT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggc > 3:11689/1‑149 (MQ=255) aTT‑CGC‑GGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcggcg > 2:38589/1‑149 (MQ=255) cgc‑gGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc < 4:72238/148‑1 (MQ=255) c‑gGG‑TAAACC‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAg < 4:62145/149‑1 (MQ=255) | TGGAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGG‑TTTACCCGCGAATGCGGAGGTATAGCCACCATCGCATT‑CGC‑GGG‑TAAA‑C‑CGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGCCAG > NC_002947/1126498‑1126785 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |