Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,846,303:1	+A	9.8%	coding (594/1377 nt)	pfeS‑II →	histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,846,303	1	.	A	9.8%	227.1 / 16.8	61	coding (594/1377 nt)	pfeS‑II	histidine kinase
Reads supporting (aligned to +/- strand):  ref base . (29/26);  new base A (3/3);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATGCGC  >  NC_002947/1846157‑1846447                                                                                                                                                     |                                                                                                                                                 cAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑Tc                                                                                                                                                >  6:163463/1‑149 (MQ=255)   gCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCgg                                                                                                                                              <  5:163463/149‑1 (MQ=255)    cACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGc                                                                                                                                             <  6:195926/149‑1 (MQ=255)    cACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCAGGCGGG‑CCGA‑CAGCTGATCGGc                                                                                                                                             >  7:241852/1‑149 (MQ=255)            gCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTTAACCAACTTCGCGAGCAGGCCAATGCCAGGCGGG‑CCGA‑CAGCTGATCGGCCCGccag                                                                                                                                      <  4:263184/148‑3 (MQ=255)             cTGTGGCGCATGCTCACCAACGGCCTCCTCCCGGGGCTGTGCACCGTGCTGGTGTGTTTACGCCTGTCCCGCATCCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGTG‑CCGATCAGCTG‑TCGGCCCGCCTGGa                                                                                                                                    <  4:258643/149‑1 (MQ=255)                  gcgcATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCAGGCGGG‑CCGA‑CAGCTGATCGGCCCGCCAggcattg                                                                                                                                <  8:241852/148‑6 (MQ=38)                    gcATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCgcgc                                                                                                                             <  2:279216/149‑1 (MQ=255)                          tCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATc                                                                                                                       >  8:293498/1‑149 (MQ=255)                          tCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATc                                                                                                                       >  2:234170/1‑149 (MQ=255)                                aCGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCg                                                                                                                  <  1:70194/149‑1 (MQ=255)                                                    ttCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGaa                                                                                               <  8:224264/147‑1 (MQ=255)                                                         cTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCTAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGgcgc                                                                                        <  2:290934/149‑1 (MQ=255)                                                         cTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGgcgc                                                                                        <  6:134378/149‑1 (MQ=255)                                                                ttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCtt                                                                                 >  2:223739/1‑149 (MQ=255)                                                                ttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCtt                                                                                 >  5:131880/1‑149 (MQ=255)                                                                  gtgtgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAAc                                                                                              >  5:84284/1‑134 (MQ=255)                                                                  gtgtgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAAc                                                                                              <  6:84284/134‑1 (MQ=255)                                                                  gtgtgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCttt                                                                                <  7:293498/148‑1 (MQ=255)                                                                    gtgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                             >  2:226672/1‑149 (MQ=255)                                                                     tgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                             >  3:32982/1‑148 (MQ=255)                                                                         ggCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGAt                                                                        <  1:223739/149‑1 (MQ=255)                                                                          gCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATg                                                                       >  6:75259/1‑149 (MQ=255)                                                                                       gCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                             <  5:111526/130‑1 (MQ=255)                                                                                       gCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                             >  6:111526/1‑130 (MQ=255)                                                                                         tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGc                                                          >  2:240758/1‑147 (MQ=255)                                                                                         tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGc                                                          >  8:120930/1‑147 (MQ=255)                                                                                         tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCAGCTGc                                                          >  1:123164/1‑147 (MQ=255)                                                                                         tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCAACTGc                                                          >  1:127193/1‑147 (MQ=255)                                                                                             cGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCa                                                    >  5:92012/1‑149 (MQ=255)                                                                                              gTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCAc                                                   >  7:45084/1‑149 (MQ=255)                                                                                                  cGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTg                                               <  6:131880/149‑1 (MQ=255)                                                                                                   gCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGc                                                                     <  4:286114/126‑1 (MQ=255)                                                                                                   gCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGc                                                                     >  3:286114/1‑126 (MQ=255)                                                                                                         ccAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATg                                         >  5:59455/1‑148 (MQ=255)                                                                                                            aCTTCGCTAGCTGGCCAATGCCTGGCGGG‑CCTATCAGGTG‑TCGGCCGGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCttt                                                                                <  6:35297/106‑1 (MQ=255)                                                                                                            aCTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCttt                                                                                >  5:35297/1‑106 (MQ=255)                                                                                                            aCTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATgcagc                                     <  5:75259/149‑1 (MQ=255)                                                                                                             cTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATgcagca                                    <  1:240758/149‑1 (MQ=255)                                                                                                                  cgAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGt                               <  4:32982/149‑1 (MQ=255)                                                                                                                      cAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTtgct                           <  6:92012/149‑1 (MQ=255)                                                                                                                           cAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGcgcg                      >  7:264274/1‑149 (MQ=255)                                                                                                                           attggCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTgcgc                       >  4:221335/5‑148 (MQ=255)                                                                                                                                 caggCGGG‑CCGA‑CAGCTGATCGGCCCGCCAGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAgg                                                      <  1:194012/109‑1 (MQ=38)                                                                                                                                 caggCGGG‑CCGA‑CAGCTGATCGGCCCGCCAGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAgg                                                      >  2:194012/3‑111 (MQ=38)                                                                                                                                 cTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGt                <  2:123164/149‑1 (MQ=255)                                                                                                                                 cTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGt                <  2:127193/149‑1 (MQ=255)                                                                                                                                    gCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTcc              >  8:242488/1‑148 (MQ=255)                                                                                                                                    gCGGG‑CCGA‑CAGCTGATCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGTGCGACCTGTcc              >  1:209257/1‑148 (MQ=255)                                                                                                                                      ggg‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCAc           >  5:94440/1‑149 (MQ=255)                                                                                                                                        g‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGCCCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAACtgctgctgcat                       >  5:146673/1‑133 (MQ=255)                                                                                                                                        g‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAACtgctgctgcat                       <  6:146673/135‑3 (MQ=255)                                                                                                                                           cGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGaaa       >  7:32143/1‑149 (MQ=255)                                                                                                                                            gATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAAt      <  7:242488/149‑1 (MQ=255)                                                                                                                                            gATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAAt      >  6:25785/1‑149 (MQ=255)                                                                                                                                             aTCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATg     <  1:234170/149‑1 (MQ=255)                                                                                                                                              tCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGGGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATgc    <  8:45084/149‑1 (MQ=255)                                                                                                                                               cAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATgcg   >  6:236170/1‑149 (MQ=255)                                                                                                                                                aGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATgcgc  >  7:282724/1‑149 (MQ=255)                                                                                                                                                  cTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTtgctgc                         >  7:234901/1‑124 (MQ=255)                                                                                                                                                  cTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTtgctgc                         <  8:234901/124‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 CAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGCTGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGG‑CCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTCCCACGAAATGCGC  >  NC_002947/1846157‑1846447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑