| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,932,638:1 | +C | 100% | intergenic (‑105/+45) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 90.0% | 118.6 / 8.0 | 40 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base C (17/19); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑G‑CCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT > NC_002947/1932491‑1932781 | tGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAATCGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑G‑c > 5:184978/1‑149 (MQ=255) gcggcgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑G‑ccc < 8:311204/149‑2 (MQ=255) gAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑Tg > 1:284700/1‑149 (MQ=255) gagctacTGCACATGCGACCGCGGCATTGCAGGTCGCACGAAAATCCCAGAAAACGCTGGGGCCG‑CTTTGCGGCCCTGCGCGGGCACGCCCCCTCCTAAAACGGATATGCGCCCCCGGTCGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCaa < 4:207056/143‑1 (MQ=255) gagGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAACCCCGCTACTAAAAAACAAATTCCTCCCCTATAAGAAC‑GGTCTTT‑C‑CCGCGAAAGGA‑Tgga > 8:260162/1‑146 (MQ=255) ggACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTGCTACAACGGGTATGCGTCCCCTGTTGGAGC‑GGGG‑GT‑GCCCGCGCAGGGC‑TGGCAGGCAGccc > 8:187974/1‑149 (MQ=38) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCCCGCCGACAAAGGATATGCGTCCCCGGTAGGAGC‑GGGCTTT‑G‑CCGCGCAGGGC‑TTCAAGGCCGCCCCaaa > 2:325345/1‑149 (MQ=38) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTACACGCGGGGGCCG‑CTTTGCGGCCCTTCGCGGGCACGCCCGCTCCTACAAAGGATATGCGGCCCCTGTCGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑Tc < 7:70480/149‑1 (MQ=37) gCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGa > 5:6449/1‑149 (MQ=255) ggTCTCACGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGAt > 7:175940/1‑147 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCCAGCCCCCAGCGACAAAGGGTATGCGTGCCCGGTTGGGGCGGGGG‑TG‑G‑CCGCGAAGGGC‑GGGCAGGGCGCCCCCAAATG‑GGGCCTACGCGGGTCacggatg > 2:273491/1‑143 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATc > 7:17674/1‑149 (MQ=255) gAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCg > 5:316772/1‑149 (MQ=255) aaatTAGCATT‑AACGGTGGGGGCGTCTTTGGGGCCGTTCGCGGGAACCCCCTCCCCTACACAGTATTTGCGCCCCCTTTAGTCGG‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCgg < 4:150614/149‑1 (MQ=255) aaTAGCAGTAAACGGTGGGGCCG‑CTTTGCGGCCGTGCGGGGGAAAGCCCGCTCCTACAATGTATTTGCGCCCCCTGTTGGTGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCa < 6:6449/149‑1 (MQ=37) aaTAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCgg > 1:288352/1‑147 (MQ=255) aCGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCCCTCCAACAAAGGATATGCGTCCCCTGTAGGAGC‑GCGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCTGATCAGCGATGCGGCAGGGAGATGtt > 8:263694/1‑148 (MQ=37) ggCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCa < 8:17674/149‑1 (MQ=255) tgggccccTGCGGGGCCACGCCCGCTCAAACAACGTATATCCGCCCCTTGTAGCCGC‑GGGC‑TTCC‑CCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGa < 7:187974/143‑1 (MQ=255) tGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTGATCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 1:89119/147‑1 (MQ=255) gCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagc > 6:66754/1‑149 (MQ=255) cGGCCCTTCGGGGGAACGCCCGCTCCTACAAAGCATAGGCGCCCCCTGTAGGTGG‑GGGG‑TG‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca < 1:186422/149‑1 (MQ=255) cGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagc < 2:288352/148‑1 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTc > 3:112526/1‑149 (MQ=255) cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCa < 3:114967/113‑1 (MQ=255) cGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCa > 4:114967/1‑113 (MQ=255) cTACAAAGTATAGGCGTCCCCTGTAGGCGC‑GGGG‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGa < 1:325345/147‑1 (MQ=255) cAACGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg < 1:273491/148‑1 (MQ=255) aGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGCATGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt > 1:250818/1‑101 (MQ=38) aGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGtt < 2:250818/100‑1 (MQ=255) tGCGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCg < 4:112526/149‑1 (MQ=255) cGTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt > 4:320664/1‑149 (MQ=255) gTCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTg > 8:303164/1‑149 (MQ=255) tCCCCTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGc < 6:184978/149‑1 (MQ=255) ccTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat < 7:153346/129‑1 (MQ=255) ccTGTAGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGat > 8:153346/1‑129 (MQ=255) aGGAGC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGtt < 5:66754/148‑1 (MQ=255) gC‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTcaca < 6:316772/148‑1 (MQ=255) c‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca > 2:210850/1‑95 (MQ=255) c‑GGGC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca < 1:210850/95‑1 (MQ=255) ggC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAtt < 2:284700/149‑1 (MQ=255) gC‑TT‑GCCCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAttt > 6:141291/1‑149 (MQ=255) | TGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCG‑CTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGC‑TT‑G‑CCGCGAAGGGC‑TGCAAGGCAGCCCCAAATTG‑TCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT > NC_002947/1932491‑1932781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |