Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,136,930:1	+C	5.7%	intergenic (‑180/‑211)	fadE ← / → gstB	medium‑long chain acyl‑CoA dehydrogenase/glutathione S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,136,930	1	.	C	5.7%	284.6 / 11.1	70	intergenic (‑180/‑211)	fadE/gstB	medium‑long chain acyl‑CoA dehydrogenase/glutathione S‑transferase
Reads supporting (aligned to +/- strand):  ref base . (32/34);  new base C (2/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.42e-01

CAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCGGGCTTGCCCGCGAAGAGG  >  NC_002947/2136788‑2137077                                                                                                                                                 |                                                                                                                                                    cAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATtgat                                                                                                                                               <  2:201773/149‑1 (MQ=255) cAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATtgat                                                                                                                                               <  3:151951/149‑1 (MQ=255) cAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTGTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCGCCTACAACGGAACGTGGCGATG‑CCACCG‑ATtga                                                                                                                                                <  3:219005/148‑1 (MQ=255)        cTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCg                                                                                                                                        >  3:176073/1‑149 (MQ=255)        cTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCg                                                                                                                                        >  3:170668/1‑149 (MQ=255)           aGACGCAATGCTGTGAACGGCATGTTGAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCAGTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑TTTGATGAATGCGGTg                                                                                                                                     >  6:362167/1‑149 (MQ=255)                cAATGCTGTGAACGGCATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGACG‑CCACCGCATTCATCAAT‑CGGTggcatc                                                                                                                                <  3:209716/149‑4 (MQ=255)                            cGGCATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGaa                                                                                                                    <  3:166098/149‑1 (MQ=255)                             ggCATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAAt                                                                                                                   <  4:351896/149‑1 (MQ=255)                               cATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGa                                                                                                                 >  7:132358/1‑149 (MQ=255)                                 tGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGaa                                                                                                                                            >  3:236863/1‑120 (MQ=255)                                 tGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGaa                                                                                                                                            <  4:236863/120‑1 (MQ=255)                                        aaCAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGt                                                                                                        >  8:180624/1‑149 (MQ=255)                                           aGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTtgg                                                                                                     >  6:177902/1‑149 (MQ=255)                                            gTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTg                                                                                                                                     <  2:99546/116‑1 (MQ=255)                                            gTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTg                                                                                                                                     >  1:99546/1‑116 (MQ=255)                                            gTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTtggc                                                                                                    <  7:152676/149‑1 (MQ=255)                                              gACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAAt                                                                                                                   >  7:114747/1‑132 (MQ=255)                                              gACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAAt                                                                                                                   <  8:114747/132‑1 (MQ=255)                                              gACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAAAGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTtggctg                                                                                                  >  4:241046/1‑149 (MQ=255)                                                 aGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATGCCCTCCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTtggctggc                                                                                                <  1:279300/149‑1 (MQ=255)                                                   ggCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAgg                                                                                             >  6:306605/1‑149 (MQ=255)                                                      gTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCGCATTCATCAAT‑CGGTggcatc                                                                                                                                <  6:317792/111‑4 (MQ=38)                                                      gTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCGCATTCATCAAT‑CGGTggcatc                                                                                                                                >  5:317792/1‑108 (MQ=38)                                                          cGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGcc                                                                                      <  2:174225/149‑1 (MQ=255)                                                           ggTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCt                                                                                     <  4:170668/149‑1 (MQ=255)                                                           ggTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCt                                                                                     <  4:176073/149‑1 (MQ=255)                                                                   tttCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTgg                                                                             <  5:74373/149‑1 (MQ=255)                                                                          tCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGAcc                                                                                                               >  2:284614/1‑108 (MQ=255)                                                                          tCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGAcc                                                                                                               <  1:284614/108‑1 (MQ=255)                                                                          tCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAAc                                                                      >  6:307952/1‑149 (MQ=255)                                                                            aTTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAAGCCCTCCTAAAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCTGTGGCGGCCGCGACGGTGAATGACCGTGGGGTTGGCGGGCAGGCTCGGCCTATGTATTGTTaaaa                                                                       <  6:143220/146‑1 (MQ=255)                                                                            aTTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCggggg                                                                                                          <  7:136554/111‑1 (MQ=255)                                                                            aTTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCggggg                                                                                                          >  8:136554/1‑111 (MQ=255)                                                                                    gCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTAtgtgt                                                                               >  7:181107/1‑130 (MQ=255)                                                                                    gCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTAtgtgt                                                                               <  8:181107/130‑1 (MQ=255)                                                                                     ccTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGaa                                                           >  7:261401/1‑149 (MQ=255)                                                                                         tACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGt                                                                                                        >  6:231792/1‑100 (MQ=255)                                                                                            cGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGt                                                    <  8:261401/149‑1 (MQ=255)                                                                                            cGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGt                                                    >  8:320586/1‑149 (MQ=255)                                                                                            cGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGt                                                    <  5:307952/149‑1 (MQ=255)                                                                                              gCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATTAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGa                                                  >  7:129823/1‑149 (MQ=255)                                                                                              gCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTg                                                   >  8:319015/1‑148 (MQ=255)                                                                                               ccTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGAc                                                 >  7:327168/1‑149 (MQ=255)                                                                                                 tATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCT‑GCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGt                                               >  4:102702/1‑148 (MQ=255)                                                                                                    tCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGTCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTg                                              >  3:202546/1‑147 (MQ=255)                                                                                                           tAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATTAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACggtg                                     <  8:129823/149‑1 (MQ=255)                                                                                                              aCCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACggtggt                                   >  3:306351/1‑148 (MQ=255)                                                                                                              aCCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCT‑GCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGa                                 <  3:102702/149‑1 (MQ=255)                                                                                                               ccccTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGa                                 <  7:319015/149‑1 (MQ=255)                                                                                                                      tACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCtgtg                          >  8:103564/1‑149 (MQ=255)                                                                                                                      tACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCATATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCtgtg                          <  7:180624/149‑1 (MQ=255)                                                                                                                       aCAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTgg                         >  2:62978/1‑149 (MQ=255)                                                                                                                        cAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCGTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACggtg                                     <  3:308764/136‑1 (MQ=255)                                                                                                                        cAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGCCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGAAGTGAAGACggag                                     >  4:308764/1‑134 (MQ=255)                                                                                                                            ggAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGt                                                                           >  5:298052/1‑94 (MQ=255)                                                                                                                            ggAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGt                                                                           <  6:298052/94‑1 (MQ=255)                                                                                                                              aaCGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGTCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCggg                  <  4:202546/149‑1 (MQ=255)                                                                                                                              aaCGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCa                                                               <  2:160109/104‑1 (MQ=255)                                                                                                                              aaCGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCa                                                               >  1:160109/1‑104 (MQ=255)                                                                                                                              aaCGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCggg                  <  5:306605/149‑1 (MQ=255)                                                                                                                               aCGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCGGGc                 <  3:241046/149‑1 (MQ=255)                                                                                                                                       ccg‑CCACCGCATTCATCAAT‑CGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCGGGCTTGCCcgc         >  2:307541/3‑149 (MQ=255)                                                                                                                                         g‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCtg                            >  5:270136/1‑128 (MQ=255)                                                                                                                                         g‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCtg                            <  6:270136/128‑1 (MQ=255)                                                                                                                                            cACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGTTCAATCACCGGGGGGTTGGCTGGCAGGCTCGGCCTAGGGGTGCGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCGGGCTTGCCCGCGAag     <  5:362167/149‑1 (MQ=255)                                                                                                                                             aCCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCGGGCTTGCCCGCGaa      >  6:19409/1‑147 (MQ=255)                                                                                                                                             aCCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCGGGCTTGCCCGCGaa      >  5:92662/1‑147 (MQ=255)                                                                                                                                              ccG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCGGGCTTGCCCGCGAagag   <  5:177902/149‑1 (MQ=255)                                                                                                                                               cG‑ATTGATGAATGCGGGGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCCGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCGGGCAAGCCcgctcccaca  <  3:310031/149‑8 (MQ=255)                                                                                                                                                 |                                                                                                                                                    CAGACAGCTTAGACGCAATGCTGTGAACGGCATGTTCAGAACAGTGACAGGGCGTGGCGGTGGGAGTTTCAGCTCATTTGTTCGCCTGTACCGGCCTATTCGCGGGTAAACCCCTCCTACAACGGAACGTGGCGATG‑CCACCG‑ATTGATGAATGCGGTGGCGGCCGCGACGGTGAATGACCGGGGGGTTGGCTGGCAGGCTCGGCCTATGTGTGGGTAAAACCCACGCAGGAATACCGGTGACGTGAAGACGGTGGTGAGCCTGTGGGAGCGGGCTTGCCCGCGAAGAGG  >  NC_002947/2136788‑2137077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑