Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 307,865:1 +C 100% intergenic (+880/‑989) hslO → / → PP_0254 molecular chaperone HslO/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947307,8651.C98.0% 163.4 / ‑3.9 49intergenic (+880/‑989)hslO/PP_0254molecular chaperone HslO/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base C (20/28);  total (20/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑C‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307722‑308008
                                                                                                                                                |                                                                                                                                                
cATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgtt                                                                                                                                             <  4:182714/149‑5 (MQ=255)
         ggTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATc                                                                                                                                     <  2:167827/148‑1 (MQ=255)
                aaCTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTgacga                                                                                                                             <  4:99819/149‑1 (MQ=255)
                     ccTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGAcgacga                                                                                                                          <  1:258303/147‑1 (MQ=255)
                     ccTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAg                                                                                                                         >  7:299470/1‑148 (MQ=255)
                           gccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCg                                                                                                                                    <  4:45841/131‑1 (MQ=255)
                           gccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCg                                                                                                                                    >  3:45841/1‑131 (MQ=255)
                            ccggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTg                                                                                                                 >  5:216145/1‑149 (MQ=255)
                             cggctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTgg                                                                                                                >  7:240919/1‑149 (MQ=255)
                               gctgccgATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTgggg                                                                                                              <  4:284460/149‑1 (MQ=255)
                                      aTGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGATCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGc                                                                                                       <  8:240919/149‑1 (MQ=255)
                                         tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGtt                                                                                                    <  7:317167/149‑1 (MQ=255)
                                         tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgtta                                                                                                                                            >  6:36537/1‑104 (MQ=255)
                                         tACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑ccgtta                                                                                                                                            <  5:36537/109‑6 (MQ=255)
                                           cTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGCCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGt                                                                                                  <  4:104529/149‑1 (MQ=255)
                                              ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTggg                                                                                                               >  5:270704/1‑133 (MQ=255)
                                              ccTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTggg                                                                                                               <  6:270704/133‑1 (MQ=255)
                                                   gCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACa                                                                                          >  7:243113/1‑149 (MQ=255)
                                                     aCTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATg                                                                                        >  6:240028/1‑149 (MQ=255)
                                                         cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTgacgac                                                                                                                            <  1:54309/109‑1 (MQ=255)
                                                         cgcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTgacgac                                                                                                                            >  2:54309/1‑109 (MQ=255)
                                                          gcgGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAgg                                                                                   <  7:335866/149‑1 (MQ=255)
                                                             gCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcgg                                                                                <  4:82230/149‑1 (MQ=255)
                                                             gCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcgg                                                                                <  8:243113/149‑1 (MQ=255)
                                                              agtaCATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAggcggc                                                                               <  1:315837/146‑1 (MQ=255)
                                                               cAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgct                                                                              >  5:242228/1‑149 (MQ=255)
                                                                 actccGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGgctgc                                                                            <  1:127131/145‑1 (MQ=255)
                                                                         gaagaaGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCaa                                                                    <  6:216145/149‑1 (MQ=255)
                                                                          aagaagGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAg                                                                   <  6:242228/149‑1 (MQ=255)
                                                                                 cGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCg                                                            <  8:299470/149‑1 (MQ=255)
                                                                                   aTGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAc                                                          <  5:240028/149‑1 (MQ=255)
                                                                                     gtgACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGAcc                                                         <  6:268359/148‑1 (MQ=255)
                                                                                          cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                    >  7:255670/1‑148 (MQ=255)
                                                                                          cTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTcc                                                    >  6:160977/1‑148 (MQ=255)
                                                                                                 ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGACGGCGGCTGCTACGCCAAGTGCATCGCACTGTCCGagaagac                                            >  4:197646/1‑148 (MQ=255)
                                                                                                 ttcGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCATCAACATGGAAGGCGGCTGATACGCCAAGTGCCTCGACCGGGCCGAtaagaa                                            >  5:35833/1‑149 (MQ=255)
                                                                                                            cGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCAACGACCTGTCCGAGAAGAACGAGCCGGTc                                  <  1:201010/148‑1 (MQ=255)
                                                                                                            cGGAACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑CGGTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAATTCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATGGACCTGGCCGAGAAGAACGAGCCGGTCa                                 <  1:200810/149‑1 (MQ=255)
                                                                                                                    gCAAGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGaaa                         <  5:160977/149‑1 (MQ=255)
                                                                                                                       aGACTACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGc                       >  6:1849/1‑148 (MQ=255)
                                                                                                                           tACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGc                                                                <  2:322572/103‑1 (MQ=255)
                                                                                                                           tACCCTTTCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGc                                                                >  1:322572/1‑103 (MQ=255)
                                                                                                                                tttCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCAc             >  8:56629/1‑149 (MQ=255)
                                                                                                                                  tCGGCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACg            <  7:56629/148‑1 (MQ=255)
                                                                                                                                     gCTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGACCGAGCCGGTCATCTGGAAAGCCATCAAGCACGgcg         >  2:229646/1‑148 (MQ=255)
                                                                                                                                      cTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACgg           <  2:213735/145‑1 (MQ=255)
                                                                                                                                      cTGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACgg           >  1:213735/1‑145 (MQ=255)
                                                                                                                                       tGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                           <  6:341429/112‑1 (MQ=255)
                                                                                                                                       tGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAAc                                           >  5:341429/1‑112 (MQ=255)
                                                                                                                                       tGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGCTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc                   <  7:262014/136‑1 (MQ=255)
                                                                                                                                       tGACGAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGCTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATc                   >  8:262014/1‑136 (MQ=255)
                                                                                                                                           gAAAGCC‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACt  <  8:255670/149‑1 (MQ=255)
                                                                                                                                           aaagg‑C‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGTCGAAGGCGTTGTCTTAAACATGGAAGGCGGCTGCTACGCCAAGTGCATCTACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGc               <  2:326135/131‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
CATGTTCTCGGTGCAGAACTTCCTGCTGCCGGCTGCCGATGTACTGCCTATGCACTGCGCGGCCAACATCGGCGAAGAAGGCGATGTGACCTTGTTCTTCGGCCTGTCCGGCACTGGCAAGACTACCCTTTCGGCTGACGAAAG‑C‑GTTACCTGATCGGTGACGACGAGCACGGTTGGGGCGAAGGCGTTGTCTTCAACATGGAAGGCGGCTGCTACGCCAAGTGCATCGACCTGTCCGAGAAGAACGAGCCGGTCATCTGGAAAGCCATCAAGCACGGCGCAGTACT  >  NC_002947/307722‑308008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑