Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,681,789:1	+C	10.9%	coding (79/1431 nt)	mltD →	membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,681,789	1	.	C	10.9%	164.0 / 16.2	46	coding (79/1431 nt)	mltD	membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base . (19/22);  new base C (3/2);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

AGGCCCGGAGCGGTTTCTAGAATCGCCGAACTTTTTCGCCCGGATACCTGTATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCA  >  NC_002947/4681652‑4681936                                                                                                                                           |                                                                                                                                                    aGGCCCGGAGCGGTTTCTAGAATCGCCGAACTTTTTCGCCCGGATACCTGTATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTtggtg                                                                                                                                          <  5:40913/149‑1 (MQ=255) aGGCCCGGAGCGGTTTCTAGAATCGCCGAACTTTTTCGCCCGGATACCTGTATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTtggtg                                                                                                                                          <  1:263376/149‑1 (MQ=255)   gCCCGGAGCGGTTTCTAGAATCGCCGAACTTTTTCGCCCGGATACCTGTATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTggg                                                                                                                                        <  3:324774/149‑1 (MQ=255)                             aaCTTTTTCGCCCGGATACCTGTATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTcgac                                                                                                               >  1:329806/1‑148 (MQ=255)                                tttttCGCCCGGATACCTGTATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGc                                                                                                                                       >  2:182534/1‑121 (MQ=255)                                tttttCGCCCGGATACCTGTATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGc                                                                                                                                       <  1:182534/121‑1 (MQ=255)                                      gCCCGGATACCTGTATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATa                                                                                                     <  1:150731/149‑1 (MQ=255)                                       cccGGATACCTGTATCAGCCGATGTCTTACCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCAAGAGCACCCGTCAGCTCGACGAATCCGATAg                                                                                                    >  1:235017/1‑149 (MQ=255)                                                cTGTATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTcgcgc                                                                                           >  2:28304/1‑149 (MQ=255)                                                   tATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGca                                                                                        >  2:199933/1‑149 (MQ=255)                                                                   cccGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCC‑AATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCAc                                                                          <  4:20533/146‑1 (MQ=255)                                                                        aGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATc                                                                     >  2:281816/1‑147 (MQ=255)                                                                            gCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGca                                                                                        <  7:65464/124‑1 (MQ=255)                                                                            gCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGca                                                                                        >  8:65464/1‑124 (MQ=255)                                                                                aaCCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAAc                                                           <  6:82876/149‑1 (MQ=255)                                                                                 aCCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAAc                                                           <  2:329806/148‑1 (MQ=255)                                                                                  ccTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCt                                                         >  3:51525/1‑149 (MQ=255)                                                                                   ctctCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCTCTGGCAGCCCACCAAGGT‑GGCTGCCAGGGc                                                                                                                              >  7:85736/1‑79 (MQ=18)                                                                                   ctctCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCTCTGGCAGCCCACCAAGGT‑GGCTGCCAGGGc                                                                                                                              <  8:85736/79‑1 (MQ=18)                                                                                   ctctCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGAt                                                                      <  4:85175/135‑1 (MQ=255)                                                                                   ctctCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGAt                                                                      >  3:85175/1‑135 (MQ=255)                                                                                        aTTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTcc                                                      <  3:52929/146‑1 (MQ=255)                                                                                        aTTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCg                                                   >  3:190695/1‑149 (MQ=255)                                                                                         ttCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCaa                                                                   >  3:123123/1‑132 (MQ=255)                                                                                         ttCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCaa                                                                   <  4:123123/132‑1 (MQ=255)                                                                                            cGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCGCAAACCTTCCCCgctgc                                               >  4:59003/1‑149 (MQ=255)                                                                                             gTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTAcc                                                                                <  5:213099/115‑1 (MQ=255)                                                                                             gTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTAcc                                                                                >  6:213099/1‑115 (MQ=255)                                                                                                         cgcCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAgccggc                                  <  1:281816/149‑1 (MQ=255)                                                                                                          gcCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAgccggcc                                 <  4:190695/149‑1 (MQ=255)                                                                                                            cTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAgccggccg                                >  5:266974/1‑148 (MQ=255)                                                                                                                cccAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAg                           >  1:20689/1‑149 (MQ=255)                                                                                                                 ccAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAgcc                                     <  2:167531/138‑1 (MQ=255)                                                                                                                 ccAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAgcc                                     >  1:167531/1‑138 (MQ=255)                                                                                                                 ccAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAgcc                                     >  1:172929/1‑138 (MQ=255)                                                                                                                 ccAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAgcc                                     <  2:172929/138‑1 (MQ=255)                                                                                                                   aaaTCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCaa                                        <  1:88513/133‑1 (MQ=255)                                                                                                                   aaaTCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCaa                                        >  2:88513/1‑133 (MQ=255)                                                                                                                             gcTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGcgccgc                    <  6:321904/143‑1 (MQ=255)                                                                                                                             gcTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGcgccgc                    >  5:321904/1‑143 (MQ=255)                                                                                                                                tgctcTGGCAGCCCACCAAGGT‑GGCTGCCAGGGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCt           >  7:172966/5‑149 (MQ=255)                                                                                                                                  ctcTGGCAGCCCACCAAGGT‑GGCTGCCAGGGCACCCGTCAGCTCGACGCATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGAGCCAGCACAAACCTTCCCAGCTGCTGGTCACGCCGGCCGAACAGGCGCCGCAGGATGTCTgg         >  5:6202/3‑149 (MQ=255)                                                                                                                                  cccTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCGCAAACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTgg         <  3:59003/149‑1 (MQ=255)                                                                                                                                    cTGGCAGCCCACCAAGGT‑GGCTGCCAGGGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGa       <  8:172966/149‑1 (MQ=255)                                                                                                                                       gCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAgcg    <  2:20689/149‑1 (MQ=255)                                                                                                                                         aG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCa  <  1:28304/149‑1 (MQ=255)                                                                                                                                           |                                                                                                                                                    AGGCCCGGAGCGGTTTCTAGAATCGCCGAACTTTTTCGCCCGGATACCTGTATCAGCCGATGTCTTCCCGTAGCCGCAGAACCTCTCATTCCGTCGCCCTGACGCGCCTGGCCCAAATCAGTGCGCTGGCCCTGGCAG‑CCACCTTGGTGGGCTGCCAGAGCACCCGTCAGCTCGACGAATCCGATAGCGTTCGCGCGCACAACTACCAGGCACGGATCAAGCACAAACCTTCCCCGCTGCTGGTCAAGCCGGCCGAACAGGCGCCGCAGGATGTCTGGGAGCGCA  >  NC_002947/4681652‑4681936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑